Otto E. Lobstein

A method for the quantitative determination of lysozyme activity in blood.

The above described bacteriolytic (turbidimetric) method of determining the lysozyme activity in the blood has been proven to be reliable, reproducible and applicable simultaneously to a large number of determinations. A wide range of concentration of crystalline egg-white lysozyme which had been added to whole blood was recovered quantitatively.

A statistical comparison of the blood Lysozyme activity of normal adults and of patients with Localized and generalized carcinomatosis

The results indicate that patients with localized and/or generalized carcinoma have statistically a higher blood lysozyme activity which is neither diagnostic nor clinically significant. Attention is, however, directed to the finding that none of the 35 carcinoma patients had less than four micrograms of lysozyme per ml. of blood and that none of the normal individuals had over 12 micrograms of lysozyme per ml. of blood (Table 1). We believe this original observation significant enough to stimulate other investigations to repeat similar studies on larger series of classified carcinoma patients.

The effect of chloride concentration in the determination of lysozyme activity

The phosphate buffer used in the determination of lysozyme by the method of Smolelis and Hartsell was modified by the addition of a given concentration of NaCl. This change was shown necessary in determining the lytic activity present in gastric juice, when it was observed that very low as well as high chloride concentrations in the buffer solution alter lysozyme activity considerably.

Role of Detergent Complexes in Experimental Gastric and Duodenal Ulcers.

Summary The results obtained from mucosulf therapy of experimental ulcer for-mation show that there is a decrease in total available pepsin and in pepsin concentration, but only a decrease in total available gastric acidity and not in its concentration. Total available lysozyme was decreased but not its concentration. The volume of gastric juice was decreased while its viscosity was increased. Total available mucin was increased as was its concentration.

The effect of a detergent complex on the deactivation and rate of reappearance of lysozyme activity in gastric juice fractional samples.

Sodium alkyl sulfate combined with gastric mucin is active for over four hours as measured by lysozyme activity in gastric juice. The detergent itself has only a fleeting effect. Medication for ulcer treatment therefore is rendered more effective by feeding the complex.

The effect of detergent complex on the rate of secretion of gastric juice and its components in total pouches of the stomach.

Several analyses of the gastric juice of isolated stomach pouches are presented before and after oral administration of RD 11, a detergent complex. These analyses show a trend of inhibition of the secretion of total available pepsin, and lysozyme, but not much change in the acidity of the secretion. The viscosity increases in the post-medicated samples, as does the amount of mucin per sample. A humoral or/and nervous inhibition mechanism therefore seems to be concerned here.