Ozcan Altinel

Comparison of hyperbaric oxygen and medical ozone therapies in a rat model of experimental distal colitis

Abstract Objectives. Previous studies have shown that hyperbaric oxygen (HBO) is effective in reducing the severity of acute distal colitis (ADC). Ozone therapy (OT) reduces inflammation in several pathological conditions. We aimed to compare the effects of HBO therapy and OT in an experimental ADC rat model. Materials and methods. Forty rats were randomly divided into four groups: Sham, ADC, ADC + HBO, and ADC + OT. Rats in the sham group were given isotonic saline. In the remaining groups, ADC was created by intracolonic administration of 4% acetic acid. No treatment was given to the ADC group. The rats in the ADC + HBO and ADC + OT groups were given HBO and ozone treatments, respectively. The administration of acetic acid caused an inflammatory response in all animals. Distal colons and blood samples were obtained. Results. The histopathological score was significantly higher in the ADC group compared to the other groups. The histopathological scores in the ADC + HBO and ADC + OT groups were significantly lower compared to the ADC group (both p < 0.001). The most pronounced therapeutic effect was observed in the ADC + OT group. Malondialdehyde and neopterin levels and superoxide dismutase and glutathione peroxidase activities in the ADC group were significantly higher compared to the other groups (p < 0.001). Conclusion. Our data showed that the therapeutic effect of OT is more pronounced than that of HBO therapy. Its possible effect is by means of decreasing inflammation, edema, and oxidative stress. These findings also suggest that it is possible to improve the outcome of ADC by using ozone therapy as an adjuvant therapy.

Pentraxin 3 as a potential biomarker of acetaminophen-induced liver injury.

OBJECTIVE Overdose of acetaminophen (APAP) can lead to severe liver injury in humans and experimental animals. Pentraxin-3 (PTX-3) is produced and released by several cell types. In this study, we aimed to evaluate whether PTX-3 is a potential biomarker in the identification of APAP-induced liver injury. MATERIALS AND METHODS Thirty adult male Wistar rats were randomly divided into three groups: control, APAP-1 and APAP-2 groups. APAP-1 (1 g/kg) and APAP-2 (2 g/kg) group rats were given APAP by gastric tube. Liver tissues and blood samples were obtained for biochemical and histopathological analysis. Biochemical parameters, plasma and liver PTX-3 levels and degree of liver necrosis were measured in all groups. RESULTS APAP treatments caused necrosis in liver and accompanied by elevated liver PTX-3 levels after 48 h. In APAP-1 and APAP-2 groups when compared with control group (7.5±3.3 ng/mg protein), mean liver PTX-3 concentrations were 14.1±3.0 (p=0.032) and 28.5±8.2 (p<0.001) ng/mg protein, respectively. All rats (100%) in the APAP-2 group had the degree 3 liver necrosis. However 10%, 40% and 50% of rats had the degree 1, the degree 2 and the degree 3 liver necrosis in the APAP-1 group, respectively. The degrees of liver necrosis of the APAP-1 and APAP-2 groups were higher than the group of control (p<0.001 and p<0.001, respectively). CONCLUSIONS PTX-3 may have a role in the APAP-induced liver injury in the rats. The elevated liver PTX-3 in the APAP-induced hepatic necrosis might be a marker of acute histological liver damage. Further prospective studies are necessary to clarify the prognostic value of liver PTX-3 for prediction of histological hepatic necrosis in the APAP-induced liver injury.

Melatonin prevents peritoneal adhesions in rats

Background and Aim:  Intra‐abdominal adhesions are important postoperative complications following abdominal surgery. The adhesions that develop form the basis of more advanced pathology such as intestinal obstruction or infertility. Melatonin is secreted from the pineal gland in a circadian pattern; this molecule has potent antioxidant characteristics and has beneficial effects in many models of inflammation. The aim of this study was to evaluate the effects of melatonin on peritoneal adhesions created in rats.

Recurrent Inguinal Hernia and Reservoir Migration after Three-Piece Penile Prosthesis Implantation

Corresponding Author: Ozcan Altınel, General Surgeon. Department of Emergency Medicine, Etlik, 06018, Ankara, Turkey. Phone: +903123043076 · GSM: +905354959283 · E-mail: ozcanaltinel@yahoo.com Özet 60 yaşında sağlıklı erkek hastaya erektil disfonksiyon nedeni ile penil protez imp¬lantasyonu uygulanmadan bir yıl önce bilateral inguinal herni ameliyatı uygulan¬mıştır. Operasyondan 3 ay sonra rezervuarın yerleştirildiği sol inguinal bölgede nüks inguinal herni gözlendi. Rezervuar hem herniye ve aynı zamanda migrate idi. Penıl protez implantasyonu ameliyatlarının bir çok komplikasyonu olsada, rezer¬vuar alanında nüks inguinal herni komplikasyonu oldukça nadirdir. Penil protez rezervuarın yerleşimi ve transvers fasiyaya baskısı sonucu olası duvar zayıflıkları rekürrens olasılığını hızlandırabilirler. Cerrah, mutlaka Penile prosthesis implantas¬yonu öncesi inguinal kanalı dikkatlice muayene etmeli ve herni durumlarında tamir için tension free yöntemleri uygulanmalıdır.

Primary Cyst Hydatidosis of Pelvis with Groin Pain and Limb: A Case Report

Echinococcosis may involve all organs of body; however, primary pelvic cyst hydatid involving bone with groin and anterior hip pain is very rare. We presented a case of extensive pelvic cyst hydatidosis involving bone without pathological fracture, in a 22-years-old man. First symtoms of patient was groin and anterior hip pain and limb. The diagnosis was made clinico-radiologically and the patient was cured with enbloc resection and filling bone cement. The patient was relieved completely one year after surgery. Cyst hydatid should be included in the differential diagnosis in patients with groin and anterior hip pain, although pelvic cyst hydatoid result in urinary, genital and abdomen problems. Much hydatid disease can be cure by administration of antihelminthic drugs, however, curettage and bone cementing is good choice in patient involving bone to eliminate recurrences and bone fracture.

PERSPECTIVES FROM A ROLE-2 HOSPITAL: A 5-YEAR ACCUMULATED VASCULAR INJURY EXPERIENCE

ÖZET Rol-2 hastane cephesinden 5 yıllık damar yaralanmaları tecrübesi Giriş ve Amaç: Bu çalışmanın amacı yüksek hızlı parça tesirine bağlı ciddi damar yaralanmalarının tedavisinden elde edilen derslerin paylaşılmasıdır. Materyal ve Metod: Çalışma retrospektif vaka serisi olarak dizayn edilmiştir. Çalışmada klinik ile fotoğraf, X-ray ve CT gibi veriler birlikte değerlendiriliştir. Çalışmada savaş ile ilgili olmayan kaza, düşme vs yaralanmalar dışlanmıştır. Veriler SPSS-22 ile incelenmiştir. Sonuçlar: Tüm vasküler yaralanmaların, 33 (% 58) arter yaralanması, 24 (% 42) venöz yaralama olarak tespit edilmiştir. Genel olarak, yaralanmaların % 65’i ekstremite yaralanmalarıdır. Ekstremite yaralanmalarının 24 (% 42) alt ekstremite iken(p> 0.36) üst ekstremite, 23 (% 40)’dır.Ekstremite damar yaralanmalarının 28(%60)aynı ektremitede kırık ile birlikte tespit edilmiştir. Tartışma: Bizim çalışmamıza ekstremite damar yaralanmaları, hakimdir. Üst ekstremite damar yaralanmaları(% 42), alt ekstremite (% 40)’dır. Çalışma sadece Rolü 2 tedavileri içermektedir. Damar yaralanmaları, mortalite ve uzuv kaybı içermemektedir. Sonuç: Operasyon alanı, taktik durum, yaralanma cinsi ve fizyolojisi savaş alanında vasküler yaralanmalarda damarın bağlanması veya onarılması kararında etkilidir. Uzun süreli takipler ile büyük damar yaralanması veri sonuçları özellikle Rolü 2 seviyesinde askeri cerrahlar için daha iyi tedavi ve eğitim stratejilerini oluşturmak için analiz edilmelidir.

the factors affecting the number of lymph nodes in specimens resected for colorectal cancer

Adequate lymph node evaluation is required for proper staging of colorectal cancer, and the number of lymph nodes examined is associated with survival. In this study, we aimed to evaluate the factors affecting the number of lymph nodes retrieved from specimens of patients operated for colorectal cancer. Medical records of 320 consecutive patients with colorectal cancer were evaluated retrospectively whom had curative resection between 2002 and 2007. Variables such as age, gender, tumor localization, depth of tumor invasion, number of lymph nodes retrieved, specimen length, stage and grade of disease, type of surgery, primary/recurrence diseasei¯ presence of preoperative chemo radiotherapy (CRT), surgeon, staff surgeon, pathologist, and staff pathologist were recorded and the results were evaluated statistically. Mean number of lymph nodes retrieved was 14.98 (0 to 129) and mean metastatic lymph node number was 2.37(0-25). Tumor localization, staging, primary/recurrence disease, length of specimen, type of operation, pathologist (resident pathologist), staff pathologist, staff surgeon, presence of CRT affected statistically significiant in terms of lymph nodes harvested (p