Özgür Koru

The scolicidal effects of honey

Echinococcosis is a zoonosis that is caused by adult or larval tapeworms belonging to the genusEchinococcus. Until now, no studies have sought to determine the scolicidal effects of honey. Therefore, the present study was undertaken to explore the scolicidal effects of honey in different concentrations for various exposure times. Tubes that contained at least 500 protoscolices were supplemented with 1 %, 5%, 10%, 25%, and 50% concentrations of honey. Tubes were maintained at room temperature for 0.5, 1, 2, 3, 4, 5, and 10 min. At the end of the incubation times, the viability of protoscolices was assessed through a trypan blue exclusion test. The effective dose of honey was applied intraperitoneally to determine whether it caused an anaphylactic reaction or hyperglycemia. Honey concentrations of 10% or greater killed all protoscolices. The scolicidal effects of honey began at the end of the third minute. Honey did not cause adverse effects when applied intraperitoneally. In this study, an investigation of the scolicidal effects of honey showed that it is highly effective at a 10% concentration. On the basis of in vivo study results, the investigators concluded that honey is a potent scolicidal agent.

Evaluation on scolicidal efficacy of propolis.

Background: Propolis is a resinous material collected by bees from various plants. It has antimicrobial, antioxidative, immunostimulative, and regenerative properties. We determined the scolicidal effect of propolis in different concentrations and various exposure times. Materials and Methods: Tubes containing Echinococcus granulosus protoscoleces were supplementedwith different concentrations of ethyl alcohol extract of propolis. At the end of the incubation times, viabilityof protoscoleces was assessed by trypan blue exclusion test. We also applied 5 ml of 1 µg/ml propolis intraperitoneally for determination of side effects and mortality rates. Results: We determined that 1 µg/ml concentration of propolis killed all of the protoscoleces at the end of the 3rd minute. In the in vivo part of the study, intraperitoneal application of propolis did not cause any side effects or mortality. Conclusion: We concluded that this natural agent can be used as a potent scolicidal agent after studies which will determine in vivo efficacy and the effects on liver and biliary tree when injected directly into the common bile duct.

Determination of antimicrobial susceptibilities of clinically isolated anaerobic bacteria by E-test, ATB-ANA and agar dilution.

A total of 60 anaerobic strains were isolated from 322 clinical specimens. These isolates were tested for susceptibility to seven antibiotics (penicillin G, amoxicillin/clavulanic acid, cefoxitin, imipenem, chloramphenicol, metronidazole, clindamycin) by using ATB-ANA and Epsilometer test (E-test) strips and the results were compared with the gold standard agar dilution method. Imipenem was found as the most effective agent in vitro among the agents tested (100%). Susceptibility to penicillin G, amoxicillin/clavulanic acid, cefoxitin, chloramphenicol, metronidazole and clindamycin are 36.7%, 83.3%, 88.3%, 96.6%, 85% and 90%, respectively. E-test has showed a good correlation (r=0.62, p=0.001) statistically with the results of agar dilution (total agreement for all antibiotics changing between 90.01% and 98.45%) and a moderate correlation (r=0.45, p=0.048) with the results of ATB-ANA method (total agreement for all antibiotics changing between 75.46% and 98.76%). However, the routine use of agar dilution procedure is concluded to be cumbersome, whereas E-test method offers a reliable alternative.

A case of mucosal leishmaniasis: Mimicking intranasal tumor with perforation of septum

A 79-year-old male suffering from nasal congestion was referred to our hospital. Endoscopic examination revealed a hyperemic mass obstructing the left nasal passage. The lesions surface was smooth. The findings of imaging studies were consistent with a benign tumor despite the erosion and perforation of the septum. The lesion originated from the middle concha and was attached to it with a thin stalk. It was removed easily by endoscopic resection. Histopathology revealed significant infiltration of mononuclear inflammatory cells, mostly lymphocytes and histiocytes, into the edematous subepithelial connective tissue. High-power magnification showed numerous Leishmania amastigotes in the cytoplasm of the histiocytes. A polymerase chain reaction experiment for Leishmania also confirmed the morphological diagnosis. No relapse was observed in the 12 months after surgery and the patient was doing well.

Microbiological Analysis and Antibacterial Effects of Turkish Thyme Honey

Introduction Located in the Mediterranean, Iran-Turan and Euro-Siberian phytogeographic region, Turkey enjoys a rich floristic and faunistic diversity. The country is home to 12,476 different plants, with 31% native to the region. This wide plant diversity makes it ideal for beekeeping. Honey production from Turkey currently averages 90,000 tons annually, harvested from approximately 6 million colonies, placing Turkey second in world honey production right after China (FAO, 2014).

Lucilia sericata Larvalarının Tüm Vücut Ekstrakt Metabolitlerinin Araştırılması ve Potensiyel Antibakteriyel Etkileri

Amac: Yakin donemde, tamamlayici tip uygulamalari modern tibbin ilgi alanina girmistir. Maggot Debritman Tedavisi dunya capinda yaygin olarak kullanilan bir yontemdir. Bu yontem, ozellikle kronik yaralarin tedavisinde tavsiye edilmektedir ve dusuk maliyet, kolay uygulanabilirlik ve nadir yan etkiler gibi avantajlari bulunmaktadir, ancak yontemin etki mekanizmasi henuz tam olarak ortaya konulamamistir. Bu calismanin amaci, Lucilia sericata larvalarinin tum vucut ekstraktinin metabolitlerini ortaya koymak ve bunlarin potensiyel antibakteriyel niteligini arastirmaktir. Gerec ve Yontemler: Antibakteriyel etkinligi arastirmak icin, onceden ozel iklim odalarinda uretilmis larvalarinin tum vucut ekstraktlari, Staphylococcus aureus , Pseudomonas aeruginosa , Escherichia coli ve Enterococcus faecalis bakterileri icin agar difuzyon ve akan hucreolcer ile test edilmistir. Antibakteriyel incelemeyi takiben, iki boyutlu elektroforez ile protein arastirilmasi yapilmistir. Bulgular: S.aureus (16mm), E.coli (22mm) ve E.faecalis (14mm) icin inhibisyon alani gozlenmis ancak P.aeruginosa icinalan olusmamistir. Hucreolcer ile farkli dilusyonlarda farkli oldurme oranlari gozlenmis ve en dusuk dilusyonda (1/64), P.aeruginosa, E.faecalis, E.coli ve S.aureus icin sirasiyla%51,9, %75, %80ve %98,7 oranlari alinmistir. Iki boyutlu elektroforezde farkli molekuler agirlik (<10-260kDa) ve izoelektrik noktada (3-9) proteinler tespit edilmistir. Sonuc: Maggot ekstraklarinin test edilen suslar uzerine antibakteriyel etkisi net olarak gozlenmistir. Farkli molekuler agirlik ve izoelektrik noktada proteinler tespit edilmistir. Antibakteriyel etkinin bu proteinler tarafindan saglanmasi muhtemel olsa da, proteinlerin kutle spektrometrisi ve protein veri bankalari ile ayrica arastirilmasi gerekir.