P.B. Lynch

Influence of dietary fat and α-tocopherol supplementation on lipid oxidation in pork.

Sixty-four pigs, approximately 3 weeks old, were fed diets containing 3% beef tallow or 3% soya oil with either a basal (10-50 mg/kg diet) or supplemented (200 mg/kg diet) level of α-tocopheral acetate. In pigs fed the soya oil diet the neutral and polar lipid fractions of muscle tissue and the total lipid fraction of adipose tissue had significantly (P < 0·05) higher C18:2/C18:1 ratios when compared to pigs fed the tallow diet. Muscle from pigs fed the soya oil diet was significantly more susceptible (P < 0·05) to lipid oxidation than muscle from pigs fed the tallow diet. In pigs receiving the α-tocopherol supplemented diet, α-tocopherol levels were approximately 3·3, 2·8 and 2-times higher in plasma, muscle and adipose tissue, respectively, when compared to pigs fed the basal level of α-tocopherol acetate. α-Tocopherol supplementation significantly increased (P < 0·05) the oxidative stability of muscle from pigs fed both the tallow and soya oil diets.

The effect of dietary vitamin E supplementation on the quality of fresh and frozen lamb meat

The effect of dietary α-tocopheryl acetate supplementation on the uptake of α-tocopherol in ewe plasma, lamb plasma, milk, organs and muscles was investigated. The oxidative stability and colour in fresh M. longissimus dorsi and frozen M. longissimus dorsi, M. psoas major and M. gluteus medius were also investigated. Ewes (n = 12) were selected and scanned to assess pregnancy. They were divided into two groups (n = 6). The control group was fed a diet containing 20 mg α-tocopheryl acetate/kg feed/day and the supplemented group fed a diet containing 1000 mg α-tocopheryl acetate/kg feed/day, for 9 weeks ante-parturition and 3 weeks post-parturition. The lambs were weaned at 3 weeks and fed supplemented or basal feed for 10 weeks before slaughter. Plasma α-tocopherol increased significantly (p < 0.01) in ewes in the 9 weeks ante-parturition, and lamb plasma taken just before slaughter was significantly (p < 0.01) higher for the supplemented group than the basal group, following 13 weeks of supplementation. Milk α-tocopherol levels were significantly (p < 0.01) higher from ewes fed the supplemented diet at parturition and for the three weeks of supplementation post-parturition (p < 0.05). Supplementation increased the α-tocopherol levels in all tissues sampled. The α-tocopherol concentrations in M. longissimus dorsi and M. psoas major were also determined after frozen storage at -20 °C for 34 weeks. Frozen storage resulted in a significant (p < 0.01) reduction in mean α-tocopherol levels for M. longissimus dorsi but not M. psoas major. Dietary supplementation with α-tocopheryl acetate significantly (p < 0.05) increased the oxidative stability of lamb muscle. Surface colour (Hunter L, a, b) was found to be negatively correlated with metmyoglobin content. Supplementation reduced surface discolouration in refrigerated display under fluorescent light over a 6-7 day storage period. The effect was more pronounced in frozen displayed muscles than in freshly displayed samples.

Uptake of α-tocopherol in porcine plasma and tissues

The effect of feeding α-tocopheryl acetate to pigs on the rate and extent of uptake of α-tocopherol in various tissues was investigated. The rate of iron ascorbate-induced lipid peroxidation was also studied. One hundred and eight Landrace × Large White pigs were assigned at random to one of the following barley-based diets: 20 mg α-tocopheryl acetate/kg feed for up to 126 days; 20 mg/kg feed to day 91, followed by 200 mg/kg feed to day 126, or 200 mg/kg feed for up to 126 days: Pigs from each group were slaughtered at specified intervals. For pigs fed the diet supplemented with 200 mg α-tocopheryl acetate/kg feed, α-tocopherol levels increased with increasing supplementation time up to day 126 in all tissues studied. The highest levels of α-tocopherol were observed in kidney fat and subcutaneous fat (inner layer) followed by subcutaneous fat (outer layer), liver, lung, heart, kidney, with muscle and brain containing approximately the same level. The α-tocopherol concentrations in all tissues examined, from pigs fed 200 mg α-tocopheryl acetate/kg feed from weaning to day 126 were greater than those fed the supplemented diet for 35 days. Iron-induced lipid peroxidation was reduced by dietary α-tocopheryl acetate supplementation, and muscle samples from pigs supplemented for 126 days were significantly (p < 0.05) less susceptible to peroxidation compared to muscle from pigs fed the same supplemented diet for 35 days.

Effects of PSE on the quality of cooked hams

Meat quality comparisons were carried out on hams produced from pork legs showing evidence of PSE and normal pork legs. Quality measurements included drip loss, sliceability, cooking loss, TBARS analysis and colour evaluation (Hunter L, a, b). A total of 40 pork legs were selected from a commercial slaughtering plant, 20 from carcasses showing PSE and 20 from normal carcasses based on L, a, b, and pH values. Carcass pH taken between the third/fourth rib was measured 45 min, 90 min, and 24 h after slaughter. Using a Minolta colorimeter (Hunter L, a, b,), colour of the Longissimus dorsi muscle was measured 24 h after slaughter. PSE had an adverse effect on cooked hams in comparison to normal cooked hams with respect to driploss (P<0.01), cooking loss (P<0.01), sliceability (P<0.01), colour (P<0.05) and lipid oxidation (P<0.05). Cooked hams manufactured from severe PSE pork in this study had an estimated financial loss of 50% (IRL£4.90) in comparison to those manufactured from normal pork (IRL£9.80).

Effect of pre- and post-weaning management on subsequent pig performance to slaughter and carcass quality

The aim of this study was to assess the effect of weaning weight and post-weaning diet on the performance of weaned pigs. In experiment 1, 30 litters with more than 10 pigs born alive per litter were selected. At 11 days of age, pigs of average weight for the litter were removed from 15 litters so that eight pigs remained per sow. These litters were given access to creep food (16·5 MJ digestible energy (DE) per kg and 18·7 g/kg lysine). The remaining litters were left complete and were not given creep food. Pigs were weaned at 28 days of age and pairs of pigs (a male and a female littermate of similar weight) were formed from each litter (no. = 54 pairs). Pairs were blocked on the basis of litter origin and weight and assigned at random to one of the following treatments: (1) 10 kg starter diet (16·1 MJ DE per kg and 17·4 g/kg lysine) followed by link diet (15·3 MJ DE per kg and 15·0 g/kg lysine) to 27 days (high dietary regimen; HDR); or (2) 4 kg starter diet, 10 kg link diet and weaner diet to 27 days (low dietary regimen; LDR). Thereafter pigs were given common diets to slaughter at about 95·6 kg live weight. On experiment 2, four pigs (two light and two heavy) were taken from each of 32 litters (no. = 128 pigs; age = 22 days), blocked on sex, litter origin, and weaning weight and within weight category individually assigned at random to two dietary treatments: (1) high density diet (16·1 MJ DE per kg and 17·4 g/kg lysine); and (2) low density diet (15·1 MJ DE per kg and 15·7 g/kg lysine). The duration of the experiment was 26 days. In experiment 1, reducing litter size and creep feeding increased weaning weight by 0·6 kg (P 0·05). This weight advantage at weaning was lost by day 14 post weaning (P > 0·05). From day 0 to day 27 post weaning daily gain was 472 and 427 g/day (s.e.12·8; P 0·05) and food conversion efficiency was 1·26 and 1·36 g/g (s.e. 0·026; P 0·05) for HDR and LDR, respectively. In experiment 2, weaning weight was 7·1 and 5·8 kg (s.e. 0·08; P 0·01) and pig weight at day 26 post weaning was 17·5 and 15·4 kg (s.e.0·23; P 0·01) for heavy and light weight categories, respectively. In the period from day 0 to 26, food intake was 440 and 396 g/day (s.e. 8·0; P 0·01) and daily gain was 389 and 355 g/day (s.e. 8·0; P

The distribution of dietary vitamin E in the muscles of the porcine carcass

The objectives of this study were to determine the effect of dietary vitamin E supplementation on tissue α-tocopherol levels in muscles in the porcine carcass. Pigs (n = 9), were selected at random and divided into three groups (n = 3) and fed diets containing 0 (basal), 20, 160 mg dl-α-tocopheryl acetate/kg feed for a period of 130 days prior to slaughter. After slaughter carcasses were split centrally and chilled at 4 °C × 24 h. Muscles (n = 37) were identified and removed from the left side of each animal for each dietary group and stored at -20 °C until required. The mean α-tocopherol levels in pork muscle tissue were significantly (p < 0.05) higher in the high-supplemented group (160 mg α-tocopheryl acetate/kg of feed) compared with the low-supplemented (20 mg/kg of feed) and basal groups (unsupplemented). In the supplemented samples, the muscles of the thoracic limb (4.8-9.9 mg α-tocopherol/kg of tissue) and neck and thorax (3.7-9.2 mg/kg) contained the greatest levels of α-tocopherol compared the muscles of the pelvic limb (4-5.6 mg/kg) and back (2.5-3.5 mg/kg).

Influence of the time of year on the incidence of PSE and DFD in Irish pigmeat

The conditions of PSE (pale, soft, exudative) and DFD (dark, firm, and dry) are significant causes for downgrading of pigmeat resulting in financial loss to the slaughter plant. The objective of this study was to document the variation in quality of pigmeat from one slaughter plant over a 15-month period, based on colour, appearance and pH. During 4 days of most weeks, 30 pigs were randomly selected from the slaughter line, resulting in a total number of 4560 pigs. Muscle pH between the third and fourth rib was measured 45 min, 90 min and 24 h after slaughter. Using a Minolta colorimeter (Hunter L, a, b scale), colour of the longissimus dorsi muscle was measured 24 h after slaughter. Muscle colour was also visually scored on a scale of 1-6. Carcass pH at 24 h post-mortem varied significantly with month of slaughter (P<0.04) as did colour (Hunter L, P<0.001; Hunter a, P<0.001; Hunter b, P<0.001). It was concluded that meat quality as measured by 24 h pH and colour was poorest during the months of November and December. While this may be at least partly related to weather, it is probable that the increased slaughtering rates at this time and variable resting period before slaughter had the biggest impact.

Influence of housing system during gestation on the behaviour and welfare of gilts in farrowing crates.

There is some evidence to show that loose housing during gestation has a negative influence on the welfare of sows subsequently in farrowing crates. However, little is known about the effects of the gestation housing on the initial responses of gilts to the farrowing crate or of the effects on gilt welfare throughout lactation. The objective of this experiment was to evaluate three gestation housing systems (1) stalls (ST); (2) loose-bedded (LB) and (3) loose-unbedded (LU) pens for their effect on behaviour, heart rate and skin lesion scores of gilts in farrowing crates. During the 1st h in the farrowing crate, LB gilts were more active, while ST gilts were more vocal. Although heart rates during the 1st h did not differ significantly between treatments, LB and LU gilts had significantly higher mean heart rates during the first 5 min in the crate, compared with 35 min later. Posture changes during the first 24 h did not differ between treatments. However, there was a significant reduction in the number of posture changes made by ST gilts but not LB or LU gilts by day 8. Increases in the skin lesion score of gilts in all three treatments were observed after 24 h in the crate, further increases were observed post farrowing in both loose treatments. Although no significant differences in the skin lesion score were observed during lactation, LB gilts were weaned with lower lesion scores than LU or ST gilts. Gilts from both loose treatments experienced greater distress at first introduction to the farrowing crate. However, the change in environment also had an adverse affect on the welfare of ST gilts. Skin damage and continuing discomfort of the loose-housed gilts post farrowing suggests that they experienced more stress at parturition. Bedding during gestation had a beneficial effect on skin health in the farrowing crate that persisted until weaning.

Age-related changes in pro-inflammatory cytokines, acute phase proteins and cortisol concentrations in neonatal piglets.

Age-related changes have been described in the resting levels of cortisol and acute phase proteins in the neonatal pig. This study evaluated the plasma concentrations of the pro-inflammatory cytokines tumour necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), the acute phase proteins C-reactive protein (CRP), serum amyloid A (SAA) and haptoglobin (Hp), as well as cortisol during the first week of postnatal life in piglets. The influence of invasive managerial practices such as teeth clipping, ear notching and tail docking on possible age-related changes in the production of these inflammatory mediators was also assessed. A total of 96 piglets were selected from 24 litters at birth, and were randomly assigned to one of four sampling times over the first week of life and one of two treatments. Blood samples were taken at 1, 3, 5 or 7 days of age. Piglets were ear notched, teeth clipped and tail docked (NCD), or were left untreated (CON). Significant effects of age were found in plasma concentrations of TNF-α, SAA, Hp and cortisol (p < 0.001). Concentrations of TNF-α and Hp increased with age, and peak concentrations were found on day 5. SAA and cortisol levels were highest on day 1, decreasing gradually with age. NCD piglets tended to have higher levels of plasma Hp than CON animals (p = 0.066). However, no differences between NCD and CON piglets were found in any other parameter measured. Furthermore, age effects were not affected by these husbandry practices. These results indicate that age-related changes exist in several inflammatory mediators, and suggest that these managerial practices do not result in systemic inflammation in early postnatal life of piglets.

Pro-inflammatory cytokine and acute phase protein responses to low-dose lipopolysaccharide (LPS) challenge in pigs

The objective of this study was to establish the pro-inflammatory cytokine and acute phase protein responses to low-dose lipopolysaccharide (LPS) challenge in pigs and to determine whether these immune parameters could also be measured in saliva. Possible gender differences in the acute phase reaction were also assessed. At 6 weeks of age, 24 male and 24 female pigs were injected intraperitoneally with a single dose of 0 or 5 μg/kg live weight (LW) of LPS from Escherichia coli (treatment). Matched saliva and blood samples were taken at 0, 2, 4, 8, 12 or 24 h after treatment administration. Samples were analysed for concentrations of the pro-inflammatory cytokines tumor necrosis factor-alpha (TNF-α) and interleukin-1beta (IL-1β), the acute phase proteins C-reactive protein (CRP), serum amyloid A (SAA), haptoglobin (Hp), and cortisol. Low-dose LPS administration increased plasma levels of TNF-α ( P P P P >0·1). Treatment by time interactions showed that plasma levels of TNF-α and CRP in LPS-treated pigs peaked at 2 h ( P P P =0·056) and the response to LPS differed between genders ( P P P P