P. Bas

Effect of level of linseed on fatty acid composition of muscles and adipose tissues of lambs with emphasis on trans fatty acids.

The effects of linseed content in concentrates on the fatty acid (FA) composition of adipose tissues and muscles of lambs were studied in a 2×4 design: males (M) vs. females (F) and linseed content (0%, L0, 3%, L3, 6%, L6, 9%, L9). FA proportions were determined both on a DB-wax and on a CP-Sil column in perirenal (PR), dorsal subcutaneous (SC) adipose tissue and in longissusmus dorsi muscle (IM). No effects of linseed contents in the diet on growth performances either in male or female lambs were observed. Linseed supplementation tended to decrease the fatness score. The proportion of linolenic acid increased linearly with the linseed content in the diet, from: 0.6, 0.5, and 0.5% for L0 to 1.9, 1.6, and 1.3% for L9, in PR, SC and IM, respectively. The increase in n-3 PUFA and in total PUFA was similar to that of linolenic acid. The n-6:n-3 ratio decreased from 5.7, 5.3 and 5.8 for L0 to 1.8, 1.7 and 2.7 for L9, in PR, SC and IM, respectively. There was no change in the proportion of docosahexaenoic acid with linseed supplementation. The proportions of C18:1trans-10 and C18:1trans-11 did not vary in PR, SC and IM with linseed supplementation. The total proportion of trans-octadecenoic acid was high in each tissue type and group of lambs. C18:1trans-10 represented about half of the total trans-octadecenoic isomers. With an increase in linolenic acid, most cis- and trans-octadecenoic isomers also increased, but trans-10 and trans-11 isomers did not and cis-9 and cis-11 isomers decreased. With linseed supplementation there was a decrease in the Δ9 desaturase indices in SC.

Fat partitioning and biochemical characteristics of fatty tissues in relation to plasma metabolites and hormones in normal and double-muscled young growing bulls

Plasma metabolites and hormones, and the biochemical characteristics of four fatty tissues (FT) were studied in two groups of six normal (N) or six double-muscled (DM) Belgian Blue young growing bulls fed the same net energy amount at the same live weight and slaughtered at 10 months of age. Average daily gain and feed efficiency did not significantly differ between the two groups. However, the DM bulls exhibited a higher proportion of muscles (+22%, P < 0.01) and a reduced proportion of fat (-49%, P < 0.01) mainly in the subcutaneous FT (-80%, P < 0.05). Triiodothyronine, insulin and glucose plasma concentrations tended to be lower in DM bulls (-24%, P < 0.02; -27%, P = 0.14; -7%, P = 0.06, respectively) and were positively related to the higher fat development in N bulls. From the results of total protein. DNA, lipid and TG contents of FT, it appeared that a reduction in fat storage per fat cell (hypotrophy) or a reduction in total fat cell number (hypoplasia) could explain, in DM bulls, two-thirds and one-third of the reduction of perirenal and subcutaneous FT weights, respectively, as compared to N bulls. In contrast, either hypotrophy or hypoplasia was the main cause of omental or intermuscular FT weight reduction in DM animals.

Effect of feeding on fatty acid composition of muscles and adipose tissues in young goats raised in the Argan tree forest of Morocco

The characteristics of meat from goats raised in the Argan tree forest in the south-western part of Morocco were studied in two experiments. Each experiment was carried out on three groups of six male goats. They were reared either indoors (IC) and fed concentrate or outdoors where they grazed in the Argan tree forest and were offered concentrate (OC) or only Argan fruit pulp (OS). Outdoor-raised goats had a very low growth rate in the first experiment (34 g/d), whereas it was relatively high in the second experiment (64 g/d) in which herbaceous vegetation availability was higher. In each experiment, omental (OM) and perirenal (PR) weights were the lowest in the two groups of goats raised outdoors. Lipid and cholesterol contents in muscles were lower in goats raised in the Argan tree forest than in those raised indoors. Outdoor-raised goats had particularly high proportions of odd-chain fatty acids (FA), branched-chain (FA) of the iso and anteiso series in adipose tissues and muscles and higher n-6 and n-3 polyunsaturated FA (PUFA) proportions and a lower n-6:n-3 PUFA ratio than indoor-raised goats. Indoor-raised goats had a higher proportion of palmitic acid and a lower proportion of archidic acid than outdoor-raised goats. In indoor-raised goats, the proportions of Δ9 straight-chain mono-unsaturated fatty acids (MUFA) (C(16:1n-7) and cis C(18:1n-9)) were relatively higher than those of total MUFA, because of lower percentages of some MUFA, such as C(16:1n-9) and other isomers of oleic acid. The low fat content and FA profile of the meat from the goats reared in the Argan tree forest conveyed beneficial characteristics to this meat in regards to human health. The relatively high contents of branched-chain FA of iso and anteiso series and PUFA in the meat from goats raised in the Argan tree forest could be considered as a sign of typicity.

The effect of maternal linseed supplementation and/or lamb linseed supplementation on muscle and subcutaneous adipose tissue fatty acid composition of indoor lambs

Eighty eight lambs were used in a 2×2 factorial arrangement 1) to investigate the effect of maternal dietary linseed supplementation and/or lamb linseed supplemented concentrate on growth performance, carcass fat quality and fatty acid (FA) composition of muscle and dorsal adipose tissue of indoor lambs 2) to study the relationships between subcutaneous fat quality and FA composition. Feeding linseed to ewes increased C18:3 n-3 (ALA) proportion in milk and therefore the ALA supply to suckling lambs. However, ALA and n-3 polyunsaturated FA (n-3 PUFA) proportions in lamb tissues were not affected. Feeding linseed to lambs during the post-weaning period significantly increased the proportions of ALA and n-3 PUFA in tissues. Softer and more colored fat was associated with a decrease in even medium-chain saturated FA and increases in odd and methyl FA proportions but not with ALA proportion in subcutaneous adipose tissue.

Quality and safety of bovine clones and their products

A multidisciplinary research programme was developed to get a scientific expertise for the quality assessment of products obtained from cloned livestock. Thirty-seven bovine Holstein female clones of five different genotypes and their products were analysed in comparison with 38 control animals obtained by conventional artificial insemination and raised under the same conditions at the same experimental farm. Animal evaluation included over 150 criteria and more than 10 000 measurements to check the physiological status and health over a 3-year period. All the parameters studied were in the normal range for age and breed, but some significant differences were detected between clone and control groups in terms of delayed onset of puberty in clones, higher neutrophil counts in haematology or lower biochemical plasma concentrations of gamma glutamyl transferase. Milk and meat analyses were conformable to expected values. We, however, found some differences in fatty acid (FA) composition of milk and muscle suggesting a possible deviation in lipid metabolism as assessed by higher delta-9 desaturase activity indexes in both milk and muscles from clones compared with controls. Repeated muscle biopsies in the semitendinosus muscle of the same animals demonstrated a higher oxidative activity in muscle of young clones (8 months of age) compared with controls, suggesting a delayed muscle maturation in clones. Nutritional evaluation of milk and meat using the rat feeding trials did not show any difference between clone and control products for food intake, growth rate, body composition of the rats, nor for possible allergenicity. Possible reactivation of bovine endogenous retroviruses (BERVs) was analysed and compared between normal and cloned cattle. As expected, these BERV sequences are not transcribed and no RNA was detected in the blood of clones, donor animals or controls; therefore, it may be assumed that the sanitary risk associated with BERV sequences is not higher in cattle derived from somatic nuclear transfer than in cattle born from conventional reproduction. Our results confirm that the quality and safety of products (milk and meat) from adult and clinically healthy cloned cattle is globally similar to normal animals. However, from a strictly biological point of view, the slightly delayed maturation we observed in the muscle of clones together with some marginal differences identified in FA composition of both muscle and milk, point to the need for more refined analysis to totally exclude any risks from the consumption of those products.

Softness and fatty acid composition of subcutaneous adipose tissue, and methylmalonic acid concentrations in the plasma of intensively reared lambs.

The aim of the present work was to study the relationships between lamb growth performance, soft adipose tissue and fatty acid composition of subcutaneous adipose tissue of intensively reared lambs, and to determine if the occurrence of soft fat, and of odd numbered (Odd FA) and methyl-branched-chain fatty acids in subcutaneous adipose tissue was related to plasma concentrations of methylmalonic acid (MMA). For this purpose, a sensitive gas chromatography assay to measure low plasma concentrations of MMA was developed and validated. In all, 49 male lambs were reared from 23.6 to 41.0kg. They were fed ad libitum with hay and pelleted concentrates containing either cereals (80%, C) or sugar beet pulp (70%, BP). Plasma concentrations of MMA were measured 12 days before slaughter. Softness score and fatty acid compositions of caudal adipose tissue were determined after slaughter. At the end of the trial, 75% of the carcasses had soft adipose tissue. The occurrence of soft fat appears to be related to high metabolisable energy intake per kg body weight. Soft fat was characterised by a higher water content, a lower proportion of saturated even-numbered fatty acids and higher proportions of Odd FA and methyl-branched-chain fatty acids in caudal adipose tissue, compared to firm fat. Despite high proportions of Odd FA and branched-chain fatty acids in caudal adipose tissue, plasma concentrations of MMA were low (2.02+/-1.98&mgr;mol/l). No clear relationships between MMA concentrations and soft fat or the proportions of branched-chain fatty acids other than the Iso and Anteiso acids was observed. It was concluded that the present study does not support the hypothesis that the livers capacity to metabolise MMA was exceeded.

Influence de l'état physiologique sur l'homéostase énergétique après l'injection d'insuline chez la chevrette en gestation

De l’insuline est injectée dans la veine jugulaire (0,12 UI/kg) à des chevrettes à 86 (G1, n = 5), 111 (G2, n = 4) et 142 (G3, n = 4) j de gestation et à 6 chevrettes non saillies (NG), du même âge que les chevrettes gestantes (10, 11 et 12 mois). Des échantillons de sang sont prélevés avant l’injection (t0) et 5, 10, 15, 20, 35, 49 et 81 min après le début de l’injection afin de mesurer les teneurs plasmatiques en glucose (GLU), insuline (INS), &beta;-hydroxy-butyrate (BHB) et acides gras

Évaluation de différents paramètres permettant d'estimer la teneur en lipides des chèvres laitières selon leur stade physiologique

Introduction Une première étude (Morand-Fehr et al, 1990) a permis d’évaluer différents paramètres en tant qu’estimateurs de la teneur en lipides des chèvres en pleine lactation. En raison des grandes variations des réserves corporelles au cours du cycle gestation-lactation (Chilliard et al, 1987), il était nécessaire de préciser si leur aptitude à estimer cette teneur variait selon le stade physiologique des chèvres.