P.C. Abhilash

Polymeric and Solid Lipid Nanoparticles for Sustained Release of Carbendazim and Tebuconazole in Agricultural Applications

Carbendazim (MBC) (methyl-2-benzimidazole carbamate) and tebuconazole (TBZ) ((RS)-1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol) are widely used in agriculture for the prevention and control of fungal diseases. Solid lipid nanoparticles and polymeric nanocapsules are carrier systems that offer advantages including changes in the release profiles of bioactive compounds and their transfer to the site of action, reduced losses due to leaching or degradation, and decreased toxicity in the environment and humans. The objective of this study was to prepare these two types of nanoparticle as carrier systems for a combination of TBZ and MBC, and then investigate the release profiles of the fungicides as well as the stabilities and cytotoxicities of the formulations. Both nanoparticle systems presented high association efficiency (>99%), indicating good interaction between the fungicides and the nanoparticles. The release profiles of MBC and TBZ were modified when the compounds were loaded in the nanoparticles, and cytotoxicity assays showed that encapsulation of the fungicides decreased their toxicity. These fungicide systems offer new options for the treatment and prevention of fungal diseases in plants.

Remediation and management of POPs-contaminated soils in a warming climate: challenges and perspectives.

There is a growing global priority for the remediation and management of persistent organic pollutants (POPs)-contaminated soil since these POPs are one of the toxic groups of chemical pollutants and listed under the Stockholm Convention for global elimination. They are potentially hazardous to living organism because of their higher degree of halogenations, inclination to bioaccumulate in the lipid component, and their resistance to natural degradation (Weber and Varbelow 2013; Torres et al. 2013a, b; Weber et al. 2013; Götz et al. 2013; Younas et al. 2013; Nurzhanova et al. 2013; Sun et al. 2013; Aliyeva et al. 2013; Oliaei et al. 2013; Xu et al. 2013; Vijgen et al. 2013; Miguel et al. 2013; Meire et al. 2013; Domínguez-Cortinas et al. 2013; Wu et al. 2013). Once enter into the pedosphere, these compounds can redistribute and partition into other environmental compartments by various physical, chemical, and biological processes such as adsorption onto soil particles, adsorption onto plant root tissues, volatilization, long-range atmospheric transport, long-range marine transport, microbial degradation and leaching, etc. Recently, nine additional “new” POPs were included in the Stockholm Convention POPs list so that the current list is having a total of 21 pollutants (Vijgen et al. 2011). Among the POPs, organochlorine pesticides (OCPs) constitute a major group. Despite the fact that most of these OCPs are banned or restricted for use in many countries, the contaminated soils and obsolete stock piles continue to have a significant impact on a number of ecosystems worldwide and pose a serious problem of bioaccumulation of POPs in soil to grazing animals and other livestock (Perugini et al. 2012) and the potential to pollute milk/dairy products (Konuspayeva et al. 2011; Tato et al. 2011) and eggs (Helgason et al. 2008; Leat et al. 2011). Huge amount of POPs polluted soils are found in different parts of the world (Abhilash and Singh, 2009; Vijgen et al. 2011; Weber and Varbelow 2013; Torres et al. 2013a, b, c; Weber et al. 2013; Abhilash et al. 2013a, b). Although most of the developed countries have already initiated the clean-up of POPs-contaminated sites and developed suitable methodologies for the ecotoxicological risk profiling of such POPscontaminated soils (Fisk et al. 2005; Letcher et al., 2010; Riva et al. 2011), more global and regional efforts should be taken to assess the extent of POPs contamination in developing countries including India and suitable methodologies should be framed to assess the actual risk posed by these POPscontaminated soils to humans beings, livestock, and other ecosystems (Abhilash and Yunus 2011). However, on the other hand, the burgeoning world population exerts tremendous pressure on soil for food, biomass and bioenergy production, and many more (Banwart 2011). It is projected that in order to meet the food demand of a growing population, the agriculture has to be highly intensified to meet an expected 50 % increase in food demand by 2030, and possibly by doubling by 2050 (Banwart 2011; Godfray 2010). Therefore, there is a huge cry for the protection of our soil resources from further damage and the remediation and restoration of already contaminated soils in an urgent basis. Although a lot of chemicaland engineeringResponsible editor: Philippe Garrigues

Nanoparticles Based on Chitosan as Carriers for the Combined Herbicides Imazapic and Imazapyr

The use of lower concentrations and fewer applications of herbicides is one of the prime objectives of the sustainable agriculture as it decreases the toxicity to non-targeted organisms and the risk of wider environmental contamination. In the present work, nanoparticles were developed for encapsulation of the herbicides imazapic and imazapyr. Alginate/chitosan and chitosan/tripolyphosphate nanoparticles were manufactured, and their physicochemical stability was evaluated. Determinations were made of the encapsulation efficiency and release kinetics, and the toxicity of the nanoparticles was evaluated using cytotoxicity and genotoxicity assays. The effects of herbicides and herbicide-loaded nanoparticles on soil microorganisms were studied in detail using real-time polymerase chain reactions. The nanoparticles showed an average size of 400 nm and remained stable during 30 days of storage at ambient temperature. Satisfactory encapsulation efficiencies of between 50 and 70% were achieved for both types of particles. Cytotoxicity assays showed that the encapsulated herbicides were less toxic, compared to the free compounds, and genotoxicity was decreased. Analyses of soil microbiota revealed changes in the bacteria of the soils exposed to the different treatments. Our study proves that encapsulation of the herbicides improved their mode of action and reduced their toxicity, indicating their suitability for use in future practical applications.

Solid waste management of temple floral offerings by vermicomposting using Eisenia fetida

Recycling of temple waste (TW) mainly comprising of floral offerings was done through vermitechnology using Eisenia fetida and its impact on seed germination and plant growth parameters was studied by comparing with kitchen waste (KW) and farmyard waste (FYW) vermicompost (VC). The worm biomass was found to be maximum in TW VC compared to KW and FYW VCs at both 40 and 120days old VCs. Physico-chemical analysis of worm-worked substrates showed better results in TW VC especially in terms of electrical conductivity, C/N, C/P and TK. 10% TW VC-water extract (VCE) showed stimulatory effect on germination percentage of chickpea seeds while KW and FYW VCE proved effective at higher concentration. Variation in growth parameters was also observed with change in the VC-soil ratio and TW VC showed enhanced shoot length, root length, number of secondary roots and total biomass at 12.5% VC compared to KW and FYW VC.

Integrated Approach of Agri-nanotechnology: Challenges and Future Trends

Nanotechnology representing a new frontier in modern agriculture is anticipated to become a major thrust in near future by offering potential applications. This integrating approach, i.e., agri-nanotechnology has great potential to cope with global challenges of food production/security, sustainability and climate change. However, despite the potential benefits of nanotechnology in agriculture so far, their relevance has not reached up to the field conditions. The elevating concerns about fate, transport, bioavailability, nanoparticles toxicity and inappropriateness of regulatory framework limit the complete acceptance and inclination to adopt nanotechnologies in agricultural sector. Moreover, the current research trends lack realistic approach that fail to attain comprehensive knowledge of risk assessment factors and further toxicity of nanoparticles toward agroecosystem components viz. plant, soil, soil microbiomes after their release into the environment. Hence in the present review we attempt to suggest certain key points to be addressed in the current and future agri-nanotechnology researches on the basis of recognized knowledge gaps with strong recommendation of incorporating biosynthesized nanoparticles to carry out analogous functions. In this perspective, the major points are as follows: (i) Mitigating risk assessment factors (responsible for fate, transport, behavior, bioavailability and toxicity) for alleviating the subsequent toxicity of nanoparticles. (ii) Optimizing permissible level of nanoparticles dose within the safety limits by performing dose dependent studies. (iii) Adopting realistic approach by designing the experiments in natural habitat and avoiding in vitro assays for accurate interpretation. (iv) Most importantly, translating environmental friendly and non-toxic biosynthesized nanoparticles from laboratory to field conditions for agricultural benefits.

Phytoextraction and dissipation of lindane by Spinacia oleracea L.

Remediation and management of organochlorine pesticide (OCPs) contaminated soil is becoming a global priority as they are listed in the Stockholm list of persistent organic pollutants (POPs) for global elimination. Lindane is a OCPs candidate recently included in the Stockholm list. However, India has an exemption to produce lindane for malaria control. Because of its widespread use during the last few decades, lindane contaminated soils are found in almost all parts of India. Since phytoremediation is widely acknowledged as an innovative strategy for the clean-up of contaminated soils; the present study was aimed to evaluate the phytoextraction and dissipation of lindane by a leafy vegetable Spinacia oleracea L (Spinach). The test plant was grown in different concentrations of lindane (5, 10, 15 and 20 mg kg(-1)) and harvested at 10, 30 and 45 days. At 45 days, the concentrations of lindane in root and leaf of Spinach growing in four different concentrations were reached up to 3.5, 5.4, 7.6 and 12.3 mg kg(-1) and 1.8, 2.2, 3 and 4.9 mg kg(-1), respectively. There was a significant difference (p<0.01) in the dissipation of lindane in vegetated and non-vegetated soil. Moreover, the residual lindane in four experiments was reduced to 81, 76, 69 and 61 percent, respectively. The experimental results indicate that Spinach can be used for the phytoremediation of lindane. However, more studies are required to prevent the toxicity of harvested parts.

Biogenic silver nanoparticles based on trichoderma harzianum : synthesis, characterization, toxicity evaluation and biological activity

White mold is an agricultural disease caused by the fungus Sclerotinia sclerotiorum, which affects important crops. There are different ways of controlling this organism, but none provides inhibition of its resistance structures (sclerotia). Nanotechnology offers promising applications in agricultural area. Here, silver nanoparticles were biogenically synthesized using the fungus Trichoderma harzianum and characterized. Cytotoxicity and genotoxicity were evaluated, and the nanoparticles were initially tested against white mold sclerotia. Their effects on soybean were also investigated with no effects observed. The nanoparticles showed potential against S. sclerotiorum, inhibiting sclerotia germination and mycelial growth. Nanoparticle characterization data indicated spherical morphology, satisfactory polydispersity and size distribution. Cytotoxicity and genotoxicity assays showed that the nanoparticles caused both the effects, although, the most toxic concentrations were above those applied for white mold control. Given the potential of the nanoparticles against S. sclerotiorum, we conclude that this study presents a first step for a new alternative in white mold control.

Biotechnological Advances for Restoring Degraded Land for Sustainable Development

Global land resources are under severe threat due to pollution and unsustainable land use practices. Restoring degraded land is imperative for regaining ecosystem services, such as biodiversity maintenance and nutrient and water cycling, and to meet the food, feed, fuel, and fibre requirements of present and future generations. While bioremediation is acknowledged as a promising technology for restoring polluted and degraded lands, its field potential is limited for various reasons. However, recent biotechnological advancements, including producing efficient microbial consortia, applying enzymes with higher degrees of specificity, and designing plants with specific microbial partners, are opening new prospects in remediation technology. This review provides insights into such promising ways to harness biotechnology as ecofriendly methods for remediation and restoration.

Plant Growth-Promoting Microorganisms for Environmental Sustainability

Agrochemicals used to meet the needs of a rapidly growing human population can deteriorate the quality of ecosystems and are not affordable to farmers in low-resource environments. Here, we propose the use of plant growth-promoting microorganisms (PGPMs) as a tool for sustainable food production without compromising ecosystems services.

Bioremediation for Fueling the Biobased Economy

Increasing CO2 emission, land degradation, and pollution are major environmental challenges that need urgent global attention. Remediation strategies are essential for tackling these issues concurrently. Here we propose integrating bioremediation with CO2 sequestration for revitalizing polluted land while deriving bioproducts from renewable and waste biomass for fueling a sustainable bioeconomy.