P. Castro
Agricultural Research Service
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Publication
Featured researches published by P. Castro.
Molecular Breeding | 2012
P. Castro; Belen Roman; J. Rubio; José V. Die
Normalisation to a reference gene is the most common method of internally controlling for error in quantitative PCR (qPCR) experiments. Studies based on qPCR in chickpea have been carried out using potential reference genes exclusively. Inappropriate normalisation may result in the acquisition of biologically irrelevant data. We have tested the expression of 12 candidate internal control genes in 36 samples representing different organs/developmental stages, genotypes and stress conditions. The most stably expressed genes were PUBQ, GAPDH, UBQ and bHLH, whereas 18S rRNA and EF-1a showed considerable regulation. The most suitable combination of reference genes for the particular experimental sets tested is provided. To illustrate the use of chickpea reference genes, we checked the expression of a putative defence gene in two different genotypes infected with Ascochyta rabiei (Pass.) Lab. The set of reference genes presented here will enable the more accurate and reliable normalisation of qPCR results for gene expression studies in this important legume crop. Our findings can be used as a starting point for reference gene selection in experimental conditions different from those tested here.
Archive | 2017
Juana María Serrano; R. Moreno; Santiago Gutiérrez; María Amérigo; Enrique Arias Fernández; Juan José Blázquez Resino; María Teresa Bejarano Franco; María Isabel Bonilla-Delgado; Margot Cajigas Romero; Elisa Isabel Cano Montero; Julián Chamizo González; Orlanda Díaz García; Eduardo Díaz Herráiz; Beatríz Esteban Ramiro; P. Castro; Patricia Fernández Montaño; Sergio Jorge Fernández Ortega; Juan Antonio Flores Martos; Juan Antonio García Martín; Santiago Gutiérrez-Broncano; Inmaculada Herranz Aguayo; Natalia Hipólito Ruiz; Pedro Jiménez Estévez; Pedro de la Paz Elez; María Luisa Lima; Juan Lirio Castro; Carolina Martín López; Irene Martínez Martín; Esther Mercado García; Sonia Morales Calvo
A comparative study of changes and developments resulting from economic challenges in Europe.
Euphytica | 2013
E. Madrid; Weidong Chen; P. N. Rajesh; P. Castro; T. Millán; J. Gil
Molecular Breeding | 2015
P. Castro; Jill M. Bushakra; P. Stewart; Cholani Weebadde; Dechun Wang; James F. Hancock; Chad E. Finn; James J. Luby; K. S. Lewers
Euphytica | 2011
P. Castro; J. Rubio; Adoración Cabrera; Teresa Millán; J. Gil
Euphytica | 2015
Latifeh Ali; Sarwar Azam; J. Rubio; Himabindu Kudapa; Eva Madrid; Rajeev K. Varshney; P. Castro; Weidong Chen; J. Gil; Teresa Millán
Scientia Horticulturae | 2014
P. Castro; J. Rubio; J. Gil; R. Moreno
Molecular Breeding | 2016
P. Castro; K. S. Lewers
Archive | 2017
Vicenta Rodríguez-Martín; P. Castro; Enrique Arias Fernández
Archive | 2017
P. Castro; Esther Portal Martínez; Enrique Arias Fernández
