P. Cortivo

The polymorphism of transferrin by ultrathin-layer isoelectric focusing. Tf phenotypes and TfC subtypes in the population of Padua.

The distribution of Tf phenotypes in the population of Padua was investigated by ultrathin-layer isoelectric focusing. In our sample (n = 618) nine phenotypes, Tf C1, C2, C3, C3-1, C2-1, C3-2, C1B, C2B and C1D, were observed and the following frequencies calculated: TfC1 = 0.77837; TfC2 = 0.1804; TfC3 = 0.03641; TfB = 0.0040; TfD = 0.0008. These gene frequencies have been compared to those found in other populations. Analysis of 101 mother-child pairs was in agreement with an autosomal codominant mode of inheritance.

Application of Conventional and DNA Polymorphism to Paternity Cases

Multilocus and single locus minisatellite probes have been applied to the paternity expertise for sometime. DNA profiling has been routinely used in cases of disputed paternity at the Institute of Legal Medicine of Padua since 1991.

The PGM1 polymorphism as revealed by ultrathin-layer isoelectric focusing in the population of Padua☆

The occurrence of PGM1 phenotypes in 589 samples from the population of Padua was investigated by ultrathin-layer isoelectric focusing. All ten phenotypes were observed. Frequencies of the PGM1 alleles (1+ = 0.6180; 1- = 0.1163; 2+ = 0.2122; 2- = 0.0535) have been compared to those found in other populations.

Genetic Study of Serum Orosomucoid ( ORM ) Polymorphism by Ultrathin Layer Isoelectric Focusing

Orosomucoid (ORM), or Alpha-1-acid glycoprotein, serum polymorphism, has not been extensively investigated until now,so that ,it is not routinely used in paternity testing. Genetic polymorphism of human serum orosomucoid can be demonstrated with various electrophoretic techniques such as starch gel electrophoresis after protein purification( 1 ), or immunofixation of neuraminidase treated serum ( 2 ), cross Immunoelectrophoresis ( 3 ), separator isoelectric focusing.

Tf and Alpha-1-AT typing in bloodstains by Isoelectric Focusing

Conventional staining procedure for serum protein have employed the use of Coomassie Brilliant Blue R-250, however, with this staining is impossible to detect these proteins when they are in small quantities, like in forensic samples, even if a highly sensitive method as IEF are used.

Population Genetic Studies on ASMAT (Irian-Jaya Indonesia)

The samples were obtained from individuals living in the districts around the village of AGATS in the territory of ASMAT.

Mitochondrial DNA polymorphism in 50 unrelated individuals from North Italy

Abstract The hypervariable regions HVI and HVII of mitochondrial DNA (mtDNA) were sequenced in 50 unrelated individuals living in Veneto. A total of 48 different mtDNA haplotypes for HVI region (404 bp) and 49 for HVII region (313 bp) were observed. Nucleotide diversity was 0.0116 and 0.0097, respectively.

Application of the STR Androgen Receptor (HUMARA) Polymorphism to Paternity Cases

The human Androgen Receptor gene located on chromosome X (Xcen-q13) contains an high polymorphic trinucleotide repeat (acg)n in the coding region of the first exon (1). The polymorphism, was first described by Sleddens (2) for its usefulness in the diagnosis of Androgen Intensitivity Syndromes. The Androgen Receptor polymorphism was studied by Edwards et al. (3, 4) for personal identification pourposes.

HLA-DQα Typing by DNA Amplification of Single Human Hair

One of the best characterized marker systems in the forensic identification with PCR is the HLA-DQα with the AmplitypeTM kit.

The Polymorphism of Bf and C3 in the Population of Veneto (Italy)

A genetic polymorphism of human C3 was demonstrated by Wieme and Demeulene are in 1967 (25) and later, confirmed simultaneously by Alper and Propp, Azen and Smithies (2, 5). They used electrophoretic techniques either in agarose gel or starch gel. They described a system with two codominant alleles C3S and C3F. Further studies revealed 22 less common variants which have been designated according to their electrophoretic mobility.