P.L. Reijven

Modification of the effects of blood on amino acid metabolism by intravenous isoleucine

The absence of isoleucine in the hemoglobin molecule has been suggested to contribute to increased urea production after a blood meal. To unravel the underlying mechanism, the effects of isoleucine infusion after blood ingestion in the healthy pig were studied. The isoleucine dose was chosen to induce an arterial isoleucine increase comparable to those observed for leucine or valine after blood ingestion. For the experiments, 10 female overnight-fasted pigs (20-25 kg) received 250 mL bovine erythrocytes intragastrically 1 week after catheter implantation for measuring hepatic, splanchnic, portal-drained viscera, and hindquarter fluxes of amino acids, urea, and ammonia. After the administration of erythrocytes, isoleucine or saline was administered i.v. for 6 hours. The data obtained show that the increase in arterial levels of urea and almost all amino acids was significantly greater in the control group (P less than 0.001) than in the isoleucine group. The net efflux of nearly all amino acids by the portal-drained viscera increased significantly less (P less than 0.001) in the isoleucine group. The liver uptake of amino acids increased after the blood meal, but the difference was not significant except for glutamine (P less than 0.001). Hindquarter amino acid net influx increased marginally. Splanchnic urea production increased more in the control group than in the isoleucine group (P less than 0.05). The data strongly suggest that i.v. administration of isoleucine enhanced the biological value of a blood meal, possibly by promoting amino acid retention in the portal drained viscera.

Sedentary Behavior Is Only Marginally Associated with Physical Function in Adults Aged 40-75 Years-the Maastricht Study

Background: In an aging population, regular physical activity (PA) and exercise have been recognized as important factors in maintaining physical function and thereby preventing loss of independence and disability. However, (older) adults spent the majority of their day sedentary and therefore insight into the consequences of sedentary behavior on physical function, independent of PA, is warranted. Objective: To examine the associations of objectively measured sedentary time (ST), patterns of sedentary behavior, overall PA, and higher intensity PA (HPA) with objective measures of physical function. Methods: This is a cross-sectional study in 1,932 men and women (aged 40–75 years) participating in The Maastricht Study. The activPAL3 was used to assess daily sedentary behavior: ST (h), sedentary breaks (n), prolonged (≥30 min) sedentary bouts (n), and to assess time spent in (H)PA (h). Measures of physical function included: covered distance during a 6 min walk test [6MWD (meters)], timed chair rise stand test performance [TCSTtime (seconds)], grip strength (kg kg−1), and elbow flexion and knee extension strength (Nm kg−1). Linear regression analyses were used to examine associations between daily sedentary behavior and PA with physical function. Results: Every additional hour ST was associated with shorter 6MWD [B = −2.69 m (95% CI = −4.69; −0.69)] and lower relative elbow extension strength (B = −0.01 Nm kg−1 (−0.02; 0.00). More sedentary breaks were associated with faster TCSTtime: B = −0.55 s (−0.85; −0.26). Longer average sedentary bout duration was associated with slower TCSTtime [B = 0.17 s (0.09; 0.25)] and lower knee extension strength [B = −0.01 Nm kg−1 (−0.02; 0.00)]. Every hour of PA and HPA were associated with greater 6MWD [BPA = 15.88 m (9.87; 21.89), BHPA = 40.72 m (30.18; 51.25)], faster TCSTtime [BPA = −0.55 s (−1.03; −0.07), BHPA = −2.25 s (−3.09; −1.41)], greater elbow flexion strength [BPA = 0.03 Nm kg−1 (0.01; 0.07)], [BHPA = 0.05 Nm kg−1 (0.01; 0.08)], and greater knee extension strength [BPA = 0.04 Nm kg−1 (0.01; 0.07)], [BHPA = 0.13 Nm kg−1 (0.06; 0.20)]. Conclusion: In adults aged 40–75 years, sedentary behavior appeared to be marginally associated with lower physical function, independent of HPA. This suggests that merely reducing sedentary behavior is insufficient to improve/maintain physical function. In contrast, engaging regularly in PA, in particular HPA, is important for physical function.

PP241-MON QUALITATIVE ANALYSIS OF BARRIERS AND FACILITATORS FOR NUTRITIONAL INTERVENTION IN HIP FRACTURE PATIENTS

PP241-MON QUALITATIVE ANALYSIS OF BARRIERS AND FACILITATORS FOR NUTRITIONAL INTERVENTION IN HIP FRACTURE PATIENTS J. Breedveld-Peters1, P.L. Reijven2, C.E. Wyers1, A.A. Hendrikx1, A.D. Verburg3, J.M. Schols4,5, M.H. Prins1,6, T. van der Weijden7, P.C. Dagnelie1. 1Department of Epidemiology, CAPHRI School for Public Health, Maastricht University, 2Department Clinical Dietetics, Maastricht University Medical Centre, Maastricht, 3Department of Orthopaedic Surgery, Orbis Medical Centre, Sittard, 4Department of General Practice and Department of Health Service Research, CAPHRI School for Public Health and Primary Care, Maastricht University Medical Centre, 5Manager of the Medical and Paramedical Treatment Department, Vivre, 6Department of Medical Technology Assessment, Maastricht University Medical Centre, 7Department of General Practice, CAPHRI School for Public Health and Primary Care, Maastricht University, Maastricht, Netherlands

PP065-SUN EFFECT OF NUTRITIONAL INTERVENTION ON LENGTH OF STAY, POSTOPERATIVE COMPLICATIONS, FUNCTIONAL STATUS AND MORTALITY IN HIP FRACTURE PATIENTS: A MULTI-CENTRE RANDOMISED CONTROLLED TRIAL (RCT)

PP064-SUN EFFECTS OF NUTRITIONAL INTERVENTION ON IMMUNE MARKERS IN MALNOURISHED ELDERLY F. Neelemaat1, M.A. van Bokhorst-de van der Schueren1, H.J. Bontkes2, J.C. Seidell3, S. Hougee4, A. Thijs5. 1Nutrition and Dietetics/Internal Medicine, 2Pathology, Unit Medical Immunology, VU University Medical Center, 3Health Sciences, Faculty of Earth and Life Sciences, VU University, Amsterdam, 4Nutricia Advanced Medical Nutrition, Danone Research, Centre for Specialised Nutrition, Wageningen, 5Internal Medicine, VU University Medical Center, Amsterdam, Netherlands