Pablo de Gracia
Spanish National Research Council
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Featured researches published by Pablo de Gracia.
PLOS ONE | 2011
Lucie Sawides; Pablo de Gracia; Carlos Dorronsoro; Michael A. Webster; Susana Marcos
Background The image formed by the eyes optics is inherently blurred by aberrations specific to an individuals eyes. We examined how visual coding is adapted to the optical quality of the eye. Methods and Findings We assessed the relationship between perceived blur and the retinal image blur resulting from high order aberrations in an individuals optics. Observers judged perceptual blur in a psychophysical two-alternative forced choice paradigm, on stimuli viewed through perfectly corrected optics (using a deformable mirror to compensate for the individuals aberrations). Realistic blur of different amounts and forms was computer simulated using real aberrations from a population. The blur levels perceived as best focused were close to the levels predicted by an individuals high order aberrations over a wide range of blur magnitudes, and were systematically biased when observers were instead adapted to the blur reproduced from a different observers eye. Conclusions Our results provide strong evidence that spatial vision is calibrated for the specific blur levels present in each individuals retinal image and that this adaptation at least partly reflects how spatial sensitivity is normalized in the neural coding of blur.
Journal of Vision | 2011
Lucie Sawides; Pablo de Gracia; Carlos Dorronsoro; Michael A. Webster; Susana Marcos
The perceived focus of an image can be strongly biased by prior adaptation to a blurred or sharpened image. We examined whether these adaptation effects can occur for the natural patterns of retinal image blur produced by high-order aberrations (HOAs) in the optics of the eye. Focus judgments were measured for 4 subjects to estimate in a forced choice procedure (sharp/blurred) their neutral point after adaptation to different levels of blur produced by scaled increases or decreases in their HOAs. The optical blur was simulated by convolution of the PSFs from the 4 different HOA patterns, with Zernike coefficients (excluding tilt, defocus, and astigmatism) multiplied by a factor between 0 (diffraction limited) and 2 (double amount of natural blur). Observers viewed the images through an Adaptive Optics system that corrected their aberrations and made settings under neutral adaptation to a gray field or after adapting to 5 different blur levels. All subjects adapted to changes in the level of blur imposed by HOA regardless of which observers HOA was used to generate the stimuli, with the perceived neutral point proportional to the amount of blur in the adapting image.
Journal of Vision | 2011
Pablo de Gracia; Carlos Dorronsoro; Gildas Marin; Martha Hernandez; Susana Marcos
Previous studies suggest that certain combinations of coma and astigmatism improve optical quality over astigmatism alone. We tested these theoretical predictions on 20 patients. Visual acuity (VA) was measured under best spherical correction for different conditions: low- and higher order aberrations corrected, in the presence of 0.5 D of induced astigmatism, and adding different amounts of coma to 0.5 D of astigmatism. Measurements were performed for different relative angles between coma and astigmatism and for selected conditions, also through-focus. Adding coma (0.23 μm for 6-mm pupil) to astigmatism resulted in a clear increase of VA in 6 subjects, consistently with theoretical optical predictions, while VA decreased when coma was added to astigmatism in 7 subjects. In addition, in the presence of astigmatism only, VA decreased more than 10% with respect to all aberrations corrected in 13 subjects, while VA was practically insensitive to the addition of astigmatism in 4 subjects. The effects were related to the presence of natural astigmatism and whether this was habitually corrected or uncorrected. The fact that the expected performance occurs mainly in eyes with no natural astigmatism suggests relevant neural adaptation effects in eyes normally exposed to astigmatic blur.
PLOS ONE | 2012
Maria Vinas; Lucie Sawides; Pablo de Gracia; Susana Marcos
Background The visual system adjusts to changes in the environment, as well as to changes within the observer, adapting continuously to maintain a match between visual coding and visual environment. We evaluated whether the perception of oriented blur is biased by the native astigmatism, and studied the time course of the after-effects following spectacle correction of astigmatism in habitually non-corrected astigmats. Methods and Findings We tested potential shifts of the perceptual judgments of blur orientation in 21 subjects. The psychophysical test consisted on a single interval orientation identification task in order to measure the perceived isotropic point (astigmatism level for which the image did not appear oriented to the subject) from images artificially blurred with constant blur strength (B = 1.5 D), while modifying the orientation of the blur according to the axis of natural astigmatism of the subjects. Measurements were performed after neutral (gray field) adaptation on naked eyes under full correction of low and high order aberrations. Longitudinal measurements (up to 6 months) were performed in three groups of subjects: non-astigmats and corrected and uncorrected astigmats. Uncorrected astigmats were provided with proper astigmatic correction immediately after the first session. Non-astigmats did not show significant bias in their perceived neutral point, while in astigmatic subjects the perceived neutral point was significantly biased, typically towards their axis of natural astigmatism. Previously uncorrected astigmats shifted significantly their perceived neutral point towards more isotropic images shortly (2 hours) after astigmatic correction wear, and, once stabilized, remained constant after 6 months. The shift of the perceived neutral point after correction of astigmatism was highly correlated with the amount of natural astigmatism. Conclusions Non-corrected astigmats appear to be naturally adapted to their astigmatism, and astigmatic correction significantly changes their perception of their neutral point, even after a brief period of adaptation.
Vision Research | 2010
Pablo de Gracia; Carlos Dorronsoro; Enrique Gambra; Gildas Marin; Martha Hernandez; Susana Marcos
We demonstrate that certain combinations of non-rotationally symmetric aberrations (coma and astigmatism) can improve retinal image quality over the condition with the same amount of astigmatism alone. Simulations of the retinal image quality in terms of Strehl Ratio, and measurements of Visual Acuity under controlled aberrations with adaptive optics were performed, varying defocus, astigmatism and coma. Astigmatism ranged between 0 and 1.5D. Defocus ranged typically between -1 and 1D. The amount of coma producing best retinal image quality (for a given relative angle between astigmatism and coma) was computed and the amount was found to be different from zero in all cases (except for 0D of astigmatism). For example, for a 6mm pupil, in the presence of 0.5D of astigmatism, a value of coma of 0.23mum produced (for best focus) a peak improvement in Strehl Ratio by a factor of 1.7, over having 0.5D of astigmatism alone. The improvement holds over a range of >1.5D of defocus and peak improvements were found for amounts of coma ranging from 0.15mum to 0.35mum. We measured VA under corrected high order aberrations, astigmatism alone (0.5D) and astigmatism in combination with coma (0.23mum), with and without adaptive optics correction of all the other aberrations, in two subjects. We found that the combination of coma with astigmatism improved decimal VA by a factor of 1.28 (28%) and 1.47 (47%) in both subjects, over VA with astigmatism alone when all the rest of aberrations were corrected. Nevertheless, in the presence of typical normal levels of HOA the effect of the coma/astigmatism interaction is considerably diminished.
Optometry and Vision Science | 2013
Maria Vinas; Pablo de Gracia; Carlos Dorronsoro; Lucie Sawides; Gildas Marin; Martha Hernandez; Susana Marcos
Purpose Astigmatic subjects are adapted to their astigmatism and perceptually recalibrate upon its correction. However, the extent to which prior adaptation to astigmatism affects visual performance, whether this effect is axis dependent, and the time scale of potential changes in visual performance after astigmatism correction are not known. Moreover, the effect of possible positive interactions of aberrations (astigmatism and coma) might be altered after recalibration to correction of astigmatism. Methods Visual acuity (VA) was measured in 25 subjects (astigmats and non-astigmats, corrected and uncorrected) under induction of astigmatism and combinations of astigmatism and coma while controlling subject aberrations. Astigmatism (1.00 diopter) was induced at three different orientations, the natural axis, the perpendicular orientation, and 45 degrees for astigmats and at 0, 90, and 45 degrees for non-astigmats. Experiments were also performed, adding coma (0.41 &mgr;m at a relative angle of 45 degrees) to the same mentioned astigmatism. Fourteen different conditions were measured using an 8-Alternative Forced Choice procedure with Tumbling E letters and a QUEST algorithm. Longitudinal measurements were performed up to 6 months. Uncorrected astigmats were provided with proper astigmatic correction after the first session. Results In non-astigmats, inducing astigmatism at 90 degrees, produced a statistically lower reduction in VA than at 0 or 45 degrees, whereas in astigmats, the lower decrease in VA occurred for astigmatism induced at the natural axis. Six months of astigmatic correction did not reduce the insensitivity to astigmatic induction along the natural axis. Differences after orientation of astigmatism were also found when adding coma to astigmatism. Conclusions The impact of astigmatism on VA is greatly dependent on the orientation of the induced astigmatism, even in non-astigmats. Previous experience to astigmatism plays a significant role on VA, with a strong bias toward the natural axis. In contrast to perceived isotropy, the correction of astigmatism does not shift the bias in VA from the natural axis of astigmatism.
Journal of Vision | 2011
Pablo de Gracia; Susana Marcos; Ankit Mathur; David A. Atchison
While correcting the aberrations of the eye produces large increases in retinal image contrast, the corresponding improvement factors in the contrast sensitivity function have been little explored and results are controversial. We measured the CSF of 4 subjects with and without correcting monochromatic aberrations. Monochromatic CSF measurements were performed at four orientations (0, 45, 90, and 135 deg) and at six spatial frequencies (2-30 c/deg). In two subjects, the CSF was also measured in polychromatic light. The MTF increased on average by 8 times and meridional changes in improvement were associated to individual meridional changes in the natural MTF. CSF increased on average by 1.35 times (only for the mid- and high spatial frequencies) and was lower (0.93 times) for polychromatic light. Under natural aberrations, the horizontal and vertical CSFs tended to be higher than the oblique CSFs, but the meridional differences in the CSF were partially reduced when the aberrations were corrected. The consistently lower benefit in the CSF than in the MTF of correcting aberrations suggests a significant role for the neural transfer function in the limit of contrast perception. Polychromatic aberrations play an additional role in degrading contrast, particularly in the absence of monochromatic high-order aberrations.
Journal of Vision | 2012
Lucie Sawides; Carlos Dorronsoro; Pablo de Gracia; Maria Vinas; Michael A. Webster; Susana Marcos
The image formed by the eyes optics is inherently blurred by aberrations specific to the individuals eyes. We examined to what extent judgments of perceived focus depend on the total magnitude as opposed to the specific pattern of blur introduced by the eyes high order aberrations (HOA). An Adaptive Optics system was used to simultaneously correct each subjects wave aberrations and display natural images blurred by simulated aberrations. To isolate the effects of blur magnitude, images were blurred by pure symmetric defocus, and subjects judged the level of the defocus that subjectively appeared best focused (i.e., neither too blurred nor too sharp). These settings were strongly correlated with the native blur magnitude. To isolate the effect of the HOA pattern, retinal image blur was instead maintained at a constant blur (Strehl Ratio) equal to each subjects natural blur, and subjects judged the best-focused image from pairs of images blurred by different patterns of HOA, one selected from 100 patterns, the other blurred by a reference pattern which included the subjects natural HOA, rotated HOA, or nine other HOA patterns. The percentage of images judged as best focused was not systematically higher when filtered with the subjects own HOA pattern. However, all subjects preferred their own HOA to the rotated version significantly more often (57% versus 45% on average across subjects). The representation of subjective image focus thus appears to be driven primarily by the overall amount of blur and only weakly by HOA blur orientation.
Biomedical Optics Express | 2016
Carlos Dorronsoro; Aiswaryah Radhakrishnan; Pablo de Gracia; Lucie Sawides; Susana Marcos
Bifocal contact or intraocular lenses use the principle of simultaneous vision to correct for presbyopia. A modified two-channel simultaneous vision simulator provided with an amplitude transmission spatial light modulator was used to optically simulate 14 segmented bifocal patterns (+ 3 diopters addition) with different far/near pupillary distributions of equal energy. Five subjects with paralyzed accommodation evaluated image quality and subjective preference through the segmented bifocal corrections. There are strong and systematic perceptual differences across the patterns, subjects and observation distances: 48% of the conditions evaluated were significantly preferred or rejected. Optical simulations (in terms of through-focus Strehl ratio from Hartmann-Shack aberrometry) accurately predicted the pattern producing the highest perceived quality in 4 out of 5 patients, both for far and near vision. These perceptual differences found arise primarily from optical grounds, but have an important neural component.
PLOS ONE | 2015
Pablo de Gracia; Beatriz I. Gallego; Blanca Rojas; Ana I. Ramírez; Rosa de Hoz; Juan J. Salazar; Alberto Triviño; José M. Ramírez
Proliferation of microglial cells has been considered a sign of glial activation and a hallmark of ongoing neurodegenerative diseases. Microglia activation is analyzed in animal models of different eye diseases. Numerous retinal samples are required for each of these studies to obtain relevant data of statistical significance. Because manual quantification of microglial cells is time consuming, the aim of this study was develop an algorithm for automatic identification of retinal microglia. Two groups of adult male Swiss mice were used: age-matched controls (naïve, n = 6) and mice subjected to unilateral laser-induced ocular hypertension (lasered; n = 9). In the latter group, both hypertensive eyes and contralateral untreated retinas were analyzed. Retinal whole mounts were immunostained with anti Iba-1 for detecting microglial cell populations. A new algorithm was developed in MATLAB for microglial quantification; it enabled the quantification of microglial cells in the inner and outer plexiform layers and evaluates the area of the retina occupied by Iba-1+ microglia in the nerve fiber-ganglion cell layer. The automatic method was applied to a set of 6,000 images. To validate the algorithm, mouse retinas were evaluated both manually and computationally; the program correctly assessed the number of cells (Pearson correlation R = 0.94 and R = 0.98 for the inner and outer plexiform layers respectively). Statistically significant differences in glial cell number were found between naïve, lasered eyes and contralateral eyes (P<0.05, naïve versus contralateral eyes; P<0.001, naïve versus lasered eyes and contralateral versus lasered eyes). The algorithm developed is a reliable and fast tool that can evaluate the number of microglial cells in naïve mouse retinas and in retinas exhibiting proliferation. The implementation of this new automatic method can enable faster quantification of microglial cells in retinal pathologies.