Padma Chatterjee

ANTIFUNGAL EFFECT OF CLITORIA TERNATEA L. LEAF EXTRACT ON SEEDS OF PISUM SATIVUM L. IN RELATION TO THE ACTIVITIES OF SOME ENZYMES

This paper highlights the antifungal activities of the leaf extract of Clitoria ternatea L. against Fusarium oxysporum ciceri. T he experiment al procedures include study of the activit ies of some ma rker enzymes of seeds of Pisum sativum L. T he leaf extract of C litoria showed antifun gal activity in relation to some enzyme activities. Fungus infestation increased activity level of some stress enzymes l ike catalase, peroxidase and Superoxide Dismutase which was controlled by the administration of the plant extract. Furthermore the activ ities of amylase, protease and dehydrogenase were decr eased by the fungal infestation which was overcome by application of the plant extract. The findings of the work thus supports that the formulation can be used as an a ntifungal agent against the fungus to overcome the de leterious effect caused by it on the pea seeds.

ANTIFUNGAL EFFECT OF CLITORIA TERNATEA L. LEAF EXTRACT ON SEEDS OF PISUM SATIVUM L. IN RELATION TO SOME BIOCHEMICAL PARAMETERS

D u r i n g t h e l a s t f e w d ec a d e s raising o f a n ti - h a za r d o us ec o - f r i e nd l y f o r m ul a t i on f o r t h e r a p e ut i c p u r p o s e s h a s g a i n e d i mm e n s e i m p o r t a n c e . H e n c e the present wo r k was taken to investigate any bio control measure in Clitoria ternatea L. leaf extract in relation to protecting Pisum sativum L. seeds ag ainst F u s a r i u m o x y s p o r u m attack . D ifferent types of extracts of Clitoria ternatea (viz. petroleum ether, chloroform and 50 % ethanolic extracts) was tested against Fusarium oxysporium for its antifungal activity by agar cup method . Active fraction was also tested on Pisum seed s for its efficiency to protecting seed s against infection caused by F. oxysporium . T h e e x p e r i m e n t s i n c l u d e e s t i m at i o n o f f ew ph y s i o l o g i ca l a n d b i o c h e m i ca l p a r a m e t e r s li k e s ug a r , a m i n o a c i d , p r o t e i n , p r o l i n e , ph e no l a n d n u c l e i c a c i d i n t h e h ealt h y a n d F u s a r i u m o x y s p o r u m i n f e s t ed s ee d s o f P i s u m s a t i v u m L . T h e 5 0 % e t h a no l i c l e a f e x t r a c t o f C l it o r ia s h o wed a n t i f u ng al a c ti v i t y a g ai n s t F u s a r i u m o x y s p o r u m cic e r i a n d e x t r a c t t r e a t m e n t e xh i b i t ed i n c r ea s ed l e v e l o f b i o c h e m i cal p a r a m e t e r s c o m p a r ed t o t h at o f f u n g u s i n f e s t ed s eed s e t s . T h e s t ud y r e v ea l ed t h at t h e c h a n g e in t h e l e v e l s o f s ug a r , a m i n o a c i d , p r o t e i n , p r o l i n e , p h e no l a n d n u c l e i c a c i d c a u s ed b y t h e f u n g al i n f e s tat i o n was protected b y t r ea t m e n t o f t h e i n f e s t e d s e e d s by a dm i n i s t r a t i o n o f t h e t e s t s a m p l e C . te r n a t e a L . b y p r e s o a k i n g m e t hod . From this study i t m a y i n f e r t h a t t h e 5 0 % e t h a no l i c l e af e x t r a c t o f C lit o r ia te r n a tea L . h as a n t i f u n g al a c t i v i t i e s and it can be used to protect the Pisum sativum seed from the attack of Fusarium oxysp orium . K e y w o rds : S t r e ss , ph y t o p a t h og e n , b i o c o n t r o l m e a s u r e s .