Pan Junsong

Construction of a genetic map with SRAP markers and localization of the gene responsible for the first-flower-node trait in cucumber (Cucumis sativus L.)

Abstract With, an F2 population from the cross of two cucumber inbred lines, S06 and S52, sequence‐related amplified polymorphism (SRAP) was used to construct a genetic linkage map in cucumber (Cucumis sativus L.). Sixty-four SRAP primer combinations generated 108 polymorphic bands in the F2 population analysis, The average of polymorphic bands produced by one primer pair was 1.5 and the maximum was 5. Using Mapmaker3.0, a linkage map was constructed, whhich consisted of 77 SRAP markers distributed in nine linkage groups (LOD≥3.0) and spanned 1114.2 cM with an average interval of 14.5 cM between markers. The gene for the first-flower-node trait, termed ffn, was mapped to linkage group IX, flanked by DC1EM5 and ME7EM2A at 10.3 cM and 12.1 cM distance, respectively ∗Supported by the Shanghai Municipal Scientific & Technological Commission, (Grant No. 033107019) and Shanghai Municipal Agricultural Commission (Grant No. 2003-2-1-2)

Localization of genes for lateral branch and female sex expression and construction of a molecular linkage map in cucumber (Cucumis sativus L.) with RAPD markers

A cucumber (Cucumis sativus L.) molecular linkage map, including 79 random-amplified polymorphic DNAs (RAPD) and two genes, lb for lateral branch and f for female sex expression, is constructed from a cross between a line, S52, with weak lateral growing ability and staminate from Dabieshan Mountains area in China and another line, S06, with strong lateral growing ability and gynoecious from Europe. The map contains nine linkage groups and spans 1110.0 cM with an average distance of 13.7 cM between loci. The lb locus is located in a longer linkage group LG-2 and flanked by two markers, OP-Q5-1 and BC151, in a short linkage group were found to flank f at 13.7 cM and 13.4 cM, respectively. The construction of RAPD map has paved a way for further study of the genes for lateral branch, female sex expression and other agronomic traits in cucumber. The authors contributed equally to this work Supported by the Shanghai Municipal Scientific & Technological Commission (Grant Nos. 033107019 and 02JC14036)

[Expression analysis of the unisex-determine gene M in cucumber].

Sex determination in plants has various mechanism; however, a single gene locus controlling unisexual expression is unique in the Cucurbitaceae plants, particularly cucumber (Cucumis sativus L.) and melon (Cucumis melo L.). In this study, with quantitative RT-PCR method, two sets of near-isogenic lines (NILs) were used to analyze the expression pattern of gene CsACS2 (GenBank accession number FJ529216). Additionally, chemical applications (AgNO3 and AVG) were used to investigate the effect of the plant endogenous ethylene on CsACS2 expression. Expression analysis reveals that endogenous ethylene, which might be derived from F or M itself, could activate the expression of M gene.