Paolo Domenico Parchi

Anterior cruciate ligament reconstruction with LARS™artificial ligament results at a mean follow-up of eight years

PurposeThe aim of this study was to review patients that underwent ACL reconstruction with the LARS™ ligament in the First Orthopaedic Division of Pisa University during the period between January 2003 and December 2005. MethodsTwenty-six patients were reviewed with an average follow-up of 95.3 months (7.9 years). The review protocol was articulated in three phases: (1) a subjective evaluation using three grading scales: VAS, KOOS and the Cincinnati knee rating scale, (2) a clinical and objective evaluation, and (3) a biomechanical evaluation of the knee stability.ResultsA global positive result was obtained in 92.3 % of the patients (16 optimal results and eight good results), with a fast functional recovery and a high knee stability. A global poor result was reported in two cases. In our series we did not record cases of infection or knee synovitis. We recorded only one case of mechanical graft failure. The results obtained from our study are encouraging and similar to those in the literature.ConclusionsWe conclude that the LARS™ ligament can be considered a suitable option for ACL reconstruction in carefully selected cases, especially for older patients needing a fast functional recovery.

Acromio-clavicular repair using two different techniques.

Acromio-clavicular joint (ACJ) injuries represent nearly half of all athletic shoulder injuries. Stability of this joint depends on the integrity of the acromio-clavicular and coracoclavicular ligaments. Although the traumatic acromioclavicular joint separation is a well-known topic, there are different classifications, diagnostic procedures, concepts of intervention, and a great variety of implants. In this paper, we present an overview of the recent literature about this issue and the results of a retrospective non-randomized study with 2 different techniques. At the first Orthopedic Department of University of Pisa, a retrospective study was performed starting from January 2007 to February 2011 in our electronic database. We selected patient treated with two different techniques (tight-rope® system and hook plate) by the same senior surgeon with experience in shoulder surgery. The mean Costant score was 90 for the tight-rope® system group and 75 for the hook plate group. At the final follow-up, most of the patients had returned to their preinjury level of activity. Two patients had a breakage of the fixating system. The above-mentioned techniques provided satisfactory results with no loss of reduction except in two cases. The use of the hook plate is useful in fracture-dislocation of ACJ, but this requires another surgery to remove it. On the contrary, the use of the tight-rope® system does not require a new surgery or use of expensive synthetic graft or a graft harvested from a distant donor site.

Quercetin nanocomposite as novel anticancer therapeutic: Improved efficiency and reduced toxicity

A three-functional nanocomposite was prepared by radical polymerization of methacrylic acid around carbon nanotubes in the presence of Quercetin as biologically active molecule and proposed as new anticancer therapeutic. The so-obtained hybrid material was characterized by FT-IR, Raman, SEM, TEM analyses, while the functionalization degree of 2.33 mg of Quercetin per g of composite was assessed by Folin-Ciocalteu test. Antioxidant test (DPPH and ABTS) showed that the covalent coupling did not interfere with the antioxidant properties of the flavonoid, while the anticancer activity was greatly enhanced with a recorded IC50 value much lower than free Quercetin. Cell viability tests on healthy cells demonstrated no-toxicity of the conjugate.

External fixation in pelvic fractures.

Pelvic fractures account for 4–5% of all fracturated patients, and they occur in 4–5% of politraumatized patients. In the most of the cases, they are consequent to high-energy trauma with a high percentage of lesions of other organs (cerebral, thoracic, and abdominal lesions. The most of the patients (80%) who die are dying within the first hours after trauma for a massive hemorrhagic shock. When the pelvic fracture and the patient’s hemodynamic conditions are both unstable, osteosynthesis of the fracture is mandatory. Fracture stabilization should be performed within the first hour after trauma (as soon as possible), and it should be considered as part of the resuscitation procedure. We usually make an urgent stabilization of pelvic fracture with an anterior external fixator technique. We have revised all unstable pelvic fractures treated in our department (Orthopaedic Clinic Pisa University) from 2000 up to the 2005 to determine a correct treatment protocol for these lesions. Pelvic stabilization, reducing the pelvic volume and bleeding from the stumps of fracture, determines the arrest of the hemorrhage, as evidenced by the sharp decline in the number of transfusions in postoperative period. In these cases, there is an absolute indication for an urgent pelvic stabilization. Pelvic stabilization, whether temporary or permanent, allows to control the bleeding because it (1) leads to a reduction in the volume pelvis with a containment on the retro-peritoneal hematoma (2) reduces bleeding from the fracture fragments (3) reduces motility fracture promoting the blood clotting. The stabilization of the pelvis also makes it easier to manage the patient and his mobilization for the implementation of subsequent investigations. In our experience, external fixator accounts for its characteristics the gold standard approach for the urgent stabilization of these lesions, and, for most of them, it can be used as the definitive treatment. External fixation is a quick and easy procedure for pelvic fractures stabilization for surgeons with experience with this technique.

Postoperative Spine Infections

Postoperative spinal wound infection is a potentially devastating complication after operative spinal procedures. Despite the utilization of perioperative prophylactic antibiotics in recent years and improvements in surgical technique and postoperative care, wound infection continues to compromise patients’ outcome after spinal surgery. In the modern era of pending health care reform with increasing financial constraints, the financial burden of post-operative spinal infections also deserves consideration. The aim of our work is to give to the reader an updated review of the latest achievements in prevention, risk factors, diagnosis, microbiology and treatment of postoperative spinal wound infections. A review of the scientific literature was carried out using electronic medical databases Pubmed, Google Scholar, Web of Science and Scopus for the years 1973-2012 to obtain access to all publications involving the incidence, risk factors, prevention, diagnosis, treatment of postoperative spinal wound infections. We initially identified 119 studies; of these 60 were selected. Despite all the measures intended to reduce the incidence of surgical site infections in spine surgery, these remain a common and potentially dangerous complication.

An optimal design for patient‐specific templates for pedicle spine screws placement

Currently, pedicle screws are positioned using a free‐hand technique or under fluoroscopic guidance, with error in the range 10–40%, depending on the skill of the surgeon.

Magnetic Nanoparticles for Efficient Delivery of Growth Factors: Stimulation of Peripheral Nerve Regeneration

The only clinically approved alternative to autografts for treating large peripheral nerve injuries is the use of synthetic nerve guidance conduits (NGCs), which provide physical guidance to the regenerating stump and limit scar tissue infiltration at the injury site. Several lines of evidence suggest that a potential future strategy is to combine NGCs with cellular or molecular therapies to deliver growth factors that sustain the regeneration process. However, growth factors are expensive and have a very short half-life; thus, the combination approach has not been successful. In the present paper, we proposed the immobilization of growth factors (GFs) on magnetic nanoparticles (MNPs) for the time- and space-controlled release of GFs inside the NGC. We tested the particles in a rat model of a peripheral nerve lesion. Our results revealed that the injection of a cocktail of MNPs functionalized with nerve growth factor (NGF) and with vascular endothelial growth factor (VEGF) strongly accelerate the regeneration process and the recovery of motor function compared to that obtained using the free factors. Additionally, we found that injecting MNPs in the NGC is safe and does not impair the regeneration process, and the MNPs remain in the conduit for weeks.

Effect of vitamin D in fracture healing in a child: case report.

SummaryIn this report, we describe the effect of vitamin D and calcium supplementation on fracture healing in a young boy with a hypovitaminosis D who had a radius refracture. The radiographic evaluation of the callus formation shows a clear effect of vitamin D on fracture healing in absence of additional variables.IntroductionFracture healing restores the tissue to its original physical and mechanical properties and it involves a complex multistep process that involves response to injury, intramembranous bone formation, chondrogenesis, endochondral bone formation, and bone remodeling. All this process is influenced by a variety of systemic and local factors. It is generally assumed that vitamin D plays an intimate role in healing fractures; however, very little data exists on how it does.Case ReportIn this report, the authors describe the effect of vitamin D and calcium supplementation on fracture healing in a young boy with a hypovitaminosis D who had a radius refracture.ConclusionsOur case report suggests that the hypovitaminosis D is a possible cause of inadequate fracture healing and refracture in children and it shows a clear effect of vitamin D supplementation on callus formation in the absence of additional variables.

Nanoparticles for Tendon Healing and Regeneration: Literature Review

Tendon injuries are commonly met in the emergency department. Unfortunately, tendon tissue has limited regeneration potential and usually the consequent formation of scar tissue causes inferior mechanical properties. Nanoparticles could be used in different way to improve tendon healing and regeneration, ranging from scaffolds manufacturing (increasing the strength and endurance or anti-adhesions, anti-microbial, and anti-inflammatory properties) to gene therapy. This paper aims to summarize the most relevant studies showing the potential application of nanoparticles for tendon tissue regeneration.

Human mesenchymal stromal cell-enhanced osteogenic differentiation by contact interaction with polyethylene terephthalate nanogratings

Among the very large number of polymeric materials that have been proposed in the field of orthopedics, polyethylene terephthalate (PET) is one of the most attractive thanks to its flexibility, thermal resistance, mechanical strength and durability. Several studies have been proposed that interface nano- or micro-structured surfaces with mesenchymal stromal cells (MSCs), demonstrating the potential of this technology for promoting osteogenesis. All these studies were carried out on biomaterials other than PET, which remains almost uninvestigated in terms of cell shaping, alignment and differentiation. Here, we study the effect of PET 350-depth nanogratings (NGs) with a ridge and lateral groove size of 500 nm (T1) or 1 μm (T2), on bone marrow-derived human MSC (hMSC) differentiation in relation to the osteogenic fate. We demonstrate that these substrates, especially T2, can promote the osteogenic phenotype more efficiently than standard flat surfaces and that this effect is more marked if cells are cultured in osteogenic medium than in basal medium. Finally, we show that the shape and disposition of calcium hydroxyapatite granules on the different substrates was influenced by the substrate symmetry, being more elongated and spatially organized on NGs than on flat surfaces.