Paolo Tongiorgi

Patterns of Diversity in Soft-Bodied Meiofauna: Dispersal Ability and Body Size Matter

Background Biogeographical and macroecological principles are derived from patterns of distribution in large organisms, whereas microscopic ones have often been considered uninteresting, because of their supposed wide distribution. Here, after reporting the results of an intensive faunistic survey of marine microscopic animals (meiofauna) in Northern Sardinia, we test for the effect of body size, dispersal ability, and habitat features on the patterns of distribution of several groups. Methodology/Principal Findings As a dataset we use the results of a workshop held at La Maddalena (Sardinia, Italy) in September 2010, aimed at studying selected taxa of soft-bodied meiofauna (Acoela, Annelida, Gastrotricha, Nemertodermatida, Platyhelminthes and Rotifera), in conjunction with data on the same taxa obtained during a previous workshop hosted at Tjärnö (Western Sweden) in September 2007. Using linear mixed effects models and model averaging while accounting for sampling bias and potential pseudoreplication, we found evidence that: (1) meiofaunal groups with more restricted distribution are the ones with low dispersal potential; (2) meiofaunal groups with higher probability of finding new species for science are the ones with low dispersal potential; (3) the proportion of the global species pool of each meiofaunal group present in each area at the regional scale is negatively related to body size, and positively related to their occurrence in the endobenthic habitat. Conclusion/Significance Our macroecological analysis of meiofauna, in the framework of the ubiquity hypothesis for microscopic organisms, indicates that not only body size but mostly dispersal ability and also occurrence in the endobenthic habitat are important correlates of diversity for these understudied animals, with different importance at different spatial scales. Furthermore, since the Western Mediterranean is one of the best-studied areas in the world, the large number of undescribed species (37%) highlights that the census of marine meiofauna is still very far from being complete.

Italian marine Gastrotricha. II: One new genus and ten new species of Macrodasyida

Abstract A new genus, Dendropodola, and species, D. transitionalis, are described in the family Dactylopodolidae. Three new species, Cephalodasys hadrosomus, Mesodasys adenotubulatus, and Mesodasys ischiensis, are described in the family Lepidodasyidae. And, six new species, Pseudostomella etrusca, Ptychostomella tyrrhenica, Tetranchyroderma heterotubulatum, Tetranchyroderma hypopsilancrum, Tetranchyroderma pachysomum, and Tetranchyroderma thysanophorum, are described in the family Thaumastodermatidae. All were collected in littoral and shallow sublittoral sediments along the Italian coastline of the Mediterranean Sea and its adjacent waters.

The Interrelationships of the Gastrotricha Using Nuclear Small rRNA Subunit Sequence Data, with an Interpretation Based on Morphology

Abstract Gastrotrichs are meiobenthic invertebrates of obscure origin and unclear phylogenetic alliances. Uncertainties also plague the intra-group relationship with major contrasts between the evolutionary scenarios inferred from morphology or molecules. In this study we analysed partial sequences of the 18S rDNA gene of 18 taxa (14 new and 4 published) to test morphological estimates of gastrotrich phylogeny and to verify whether controversial interrelationships from previous molecular data are due to poor sampling. Data were analysed using both maximum parsimony and maximum likelihood. MP topology was then forced to reflect published morphological estimates and the most parsimonious solutions from each constraint analysis was statistically compared against the unconstrained solution. MP analysis yielded a single tree with few nodes well supported by bootstrap resampling. These included the monophyly of the Chaetonotidae and the internal relationships of the members of this family, with Aspidiophorus appearing as the most basal member. The monophyly of the Turbanellidae was also well supported with some suggestion that its sister group might be Mesodasys . Lepidodasyidae was found to be an unnatural taxon with Lepidodasys forming a separated clade but unrelated also to the Thaumastodermatidae. With the exception of genera Lepidodasys and Neodasys , the Macrodasyida appeared to be resolved separately from the Chaetonotida, and Dactylopodola was resolved as the most basal macrodasyid. ML analysis yielded a tree not too dissimilar from MP, although Dactylopodola and Xenodasys were resolved as a clade. Statistics indicate that the output from our MP analysis is compatible with the classical view placing representatives of the two orders within two distinct evolutionary lines. Most of the constrained solutions, except the shortest, corroborate the monophyly of the two orders, whereas all five constrained solutions support also the notion that sees Neodasys as an early divergent clade along the Chaetonotida branch. Thus, results are generally compatible with the hypothesised evolutionary scenario based on morphological data, but are in contrast with previous findings from molecules. Future research should consider using the complete SSU rDNA gene sequence in their analysis and additional genes for deeper resolution.

Marine gastrotrichs from the Tuscan archipelago (Tyrrhenian Sea): I. macrodasyida, with description of three new species

Abstract Twenty‐three species of chetonotid gastrotrichs are reported from three islands of the Tuscan Archipelago (Tyrrhenian Sea, Italy). The island of Elba is the largest in the archipelago and has the most sand beaches. It possesses the greatest diversity of gastrotrich fauna, with 17 known species. The island of Capraia ranks second with 12 species, and the island of Giglio is third with 8 species. Chaetonotus napoleonicus from the island of Elba and Chaetonotus aegilonensis together with Halichaetonotus marivagus, both from the island of Capraia, are described as new species. A clarification of the diagnostic characters of C. atrox, C. dispar and C. neptuni is given. Xenotrichula carolinensis is synonymized with X. intermedia.

The effect of salinity on the chemotaxis of glass eels, Anguilla anguilla, to organic earthy and green odorants

SynopsisThe behaviour of upstream migrating glass eels to salt and brackish water solutions of 8 pure organic earthy and green odorants was investigated. In 35‰ salt water, IPMCET and D-MF are ineffective, while MMP, IBMP, MT, TMCE, ETMCE and L-MF are strongly repellent. Dissolved in brackish water, MMP and ETMCE become attractive. The strong attraction which glass eels show to earthy and green odorants emerges as the level of salinity is reduced, suggesting that these chemicals could be orienting cues in the last phase of glass eel migration.

Reproductive system and spermatozoa of Paraturbanella teissieri (Gastrotricha, Macrodasyida): implications for sperm transfer modality in Turbanellidae

Abstract. The morphology of the reproductive apparatus of several species of Turbanellidae, which are sequential hermaphrodites, has been studied for a comparison with that of other Gastrotricha Macrodasyida, which are simultaneous hermaphrodites. The common structural plan of the genital system of Turbanellidae includes two testes which extend into two sperm ducts turning anteriorly and fusing in a midventral pore, two ovaries with oocytes maturing in a cephalic direction and only one accessory organ, a seminal receptacle, provided with an external pore. A possible sperm transfer modality alternative to that described in the literature is advanced. Spermatological characters of Paraturbanella teissieri have been compared with those of the two Turbanella species studied up to date. Turbanellidae share with other Macrodasyida the general model of spermatozoon, but are the only representatives of this taxon known so far which have sperm devoid of the striated cylinder around the axoneme. Both the structure of the reproductive apparatus and the fine morphology of the spermatozoa of Turbanellidae species agree with the evolutionary view, recently supported by morphological and molecular data, which puts this taxon on a separate clade, early divergent from the stem lineage of Macrodasyida.

Italian marine Gastrotricha: III. Four new pentancrous species of the genus Tetranchyroderma (Macrodasyida, Thaumastodermatidae)

Abstract Of the thaumastodermatid gastrotrichs that were collected in the years 1989–1991, during a faunistic survey of the Italian coasts, several have been placed in the genus Tetranchyroderma. On the basis of their distinctive morphological characters, four that bear pentancres are being described as new species, three with a complete dorsal covering of ancres: T. kontosomum, T. polyprobo‐lostomum, T. tanymesatherum, and one with an incomplete covering: T. anomalopsum.

Marine and freshwater Gastrotricha from the Island of Montecristo (Tuscan Archipelago, Italy), with the description of new species

Abstract Marine and freshwater gastrotrich fauna from the Island of Montecristo (Tuscan Archipelago) is reported. Thirteen macrodasyid and six chaetonotid species were found in marine sandy sediments from four collecting sites. The new species Lepidodasys unicarenatus and Tetranchyroderma insulare ate described. Nine chaetonotid species were collected from the inland waters of the island, and the new species Chaetonotus (Schizochaetonotus) oplites is described. A comparison between the gastrotrich fauna of Montecristo and that of other islands in the archipelago and of the Tuscan littoral is drawn.

Effects of pollution on marine gastrotricha in the northwestern Adriatic Sea

The barrier islands and coast south and east of Venice, Italy, were sampled in parallel to the work of Schrom two decades ago. Where he found 49 site records for 24 species of gastrotrichs at 8 sites, 5 littoral and 3 shallow sublittoral, we found 5 site records for 2 species at 9 sites, 5 littoral and 4 shallow sublittoral. We had no site records from littoral sites; one species, Dolichodasys elongatus, occurred at all sublittoral sites. From other locations (n=15) in our present survey of the Italian coastline, where we have paired littoral: sublittoral sites, we have found 6.7 (±0.9) species in the former and 8.0 (±0.9) in the latter. The absence of gastrotrichs in samples from all five littoral sites and the dominance of D. elongatus in sublittoral sites is clear evidence as to the severity of pollution in this area. However, five species were found in a sample from the top of a longshore bar at one location, indicating that a type of local refugium exists from which future recolonization of the remaining habitat could proceed, if the input of pollutants can be reversed.

Marine gastrotrichs from the Tremiti Archipelago in the southern Adriatic Sea, with the description of two new species of Urodasys

Abstract Twenty‐four macrodasyid and thirteen chaetonotid species of marine Gastrotricha are reported. Two of these species ‐ Urodasys apuliensis and U. bucinastylis ‐ are new, while the macrodasyid genus Crasiella is reported for the first time in Italy and the Mediterranean basin. Samples were taken at depths varying from 1 to 34 metres. Altogether, the number of species found was relatively high and comparable with the range of fauna found on the major Italian islands. A possible evolution of the reproductive system in the genus Urodasys is briefly discussed.