Parthasarathi Panda

Evaluation of the Isotopic Abundance Ratio in Biofield Energy Treated Resorcinol Using Gas Chromatography-Mass Spectrometry Technique

The stable isotope ratio analysis is widely used in several scientific fields such as agricultural, food authenticity, biochemistry, metabolism, medical research, etc. Resorcinol is one of the most versatile chemicals used for the synthesis of several pharmaceuticals, dyes, polymers, organic compounds, etc. The current research work was designed to investigate the impact of the biofield energy treatment on the isotopic abundance ratios of 13C/12C or 2H/1H or 17O/16O (PM+1/PM) and 18O/16O (PM+2/PM) in resorcinol using Gas chromatograph - mass spectrometry (GC-MS) technique. Resorcinol was divided into two parts - one part was control and another part was considered as biofield energy treated sample. The biofield energy treatment was accomplished through unique biofield energy transmission by Mr. Mahendra Kumar Trivedi (also called as The Trivedi Effect®). T1, T2, T3, and T4 were denoted by different time interval analysis of the biofield treated resorcinol in order to understand the influence of the biofield energy treatment on isotopic abundance ratio with respect to the time. The GC-MS spectra of the both control and biofield treated resorcinol exhibited the presence of molecular ion peak [M+] at m/z 110 (calculated 110.04 for C6H6O2) along with major fragmented peaks at m/z 82, 81, 69, 53, and 39. The relative peak intensities of the fragmented ions in biofield treated resorcinol (particularly T2) was significantly changed with respect to the control sample. The stable isotope ratio analysis in resorcinol using GC-MS revealed that the percentage change of the isotopic abundance ratio of PM+1/PM was increased in the biofield treated resorcinol at T1, T2, T3 and T4 by 1.77%, 165.73%, 0.74%, and 6.79%, respectively with respect to the control sample. Consequently, the isotopic abundance ratio of PM+2/PM in the biofield treated resorcinol at T2, T3, and T4 were enhanced by 170.77%, 3.08%, and 12.31%, respectively with respect to the control sample. Briefly, 13C, 2H, 17O contributions from (C6H6O2)+ to m/z 111 and 18O contribution from (C6H6O2)+ to m/z 112 for the biofield treated resorcinol at T2 and T4 were significantly altered as compared to the control sample. For this reasons, biofield treated resorcinol might exhibit altered physicochemical properties like diffusion velocity, mobility and evaporation rate, reaction rate, binding energy, and stability. Biofield treated resorcinol could be valuable in pharmaceutical and chemical industries as intermediates during the preparation of pharmaceuticals and chemical compounds by altering its physicochemical properties, the reaction rate and selectivity, the study of the reaction mechanism and facilitating in designing extremely effective and specific enzyme inhibitors.

A Systematic Study of the Biofield Energy Healing Treatment on Physicochemical, Thermal, Structural, and Behavioral Properties of Magnesium Gluconate

Magnesium gluconate is a pharmaceutical/nutraceutical compound used as a source of magnesium ion. The recent study described the impact of The Trivedi Effect®-Energy of Consciousness Healing Treatment on magnesium gluconate for the variation in physicochemical, structural, thermal and behavioral properties using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Magnesium gluconate was divided into two parts – one part was control without any Biofield Energy Treatment, while another part was treated with The Trivedi Effect®-Energy of Consciousness Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The PXRD analysis exhibited that the crystallite size of the treated sample was remarkably altered from -70% to 130% compared with the control sample. The average crystallite size was significantly decreased by 23.74% in the treated sample compared to the control sample. Biofield Energy Healing Treatment significantly reduced the particle size of magnesium gluconate at d10, d50, and d90 values by 12.15%, 8.98% and 15.35%, respectively compared to the control sample. The surface area analysis showed that surface area of the treated sample was significantly increased by 11.76% compared with the control sample. The FT-IR and UV-vis analysis displayed that structure of the magnesium gluconate persisted identical in both the treated and control samples. The TGA analysis exhibited four steps thermal degradation in both samples and the total weight loss of the Biofield Energy Treated sample was reduced by 0.19% compared with the control sample. The melting temperature of the Biofield Energy Treated sample (171.25oC) was slightly (0.16%) higher from the control sample (170.97oC). The latent heat of fusion was significantly decreased by 7.76% in the treated sample compared to the control sample. The TGA and DSC analysis revealed that the thermal stability of the treated sample was enhanced compared with the control sample. The current study revealed that The Trivedi Effect®-Energy of Consciousness Healing Treatment might produce a new polymorphic form of magnesium gluconate, which could be more soluble and bioavailable along with improved thermal stability compared with the untreated compound. The Biofield Treated sample could be more stable during manufacturing, delivery or storage conditions than the untreated sample. Hence, The Trivedi Effect® Treated magnesium gluconate would be very useful to design better nutraceutical/pharmaceutical formulations that might offer better therapeutic responses against inflammatory diseases, immunological disorders, stress, aging, and other chronic infections.

Metabolite Profiling of Withania somnifera Roots Hydroalcoholic Extract Using LC-MS, GC-MS and NMR Spectroscopy

Ashwagandha (Withania somnifera) is a very well‐known herbal medicine and it was well studied for its active metabolites throughout the World. Although, nearly 40 withanolides were isolated from W. somnifera root extract, still there is remaining unidentified metabolites due to very low abundance and geographical variation. Advanced separation technology with online identification by mass and nuclear magnetic resonance (NMR) are nowadays used to find out the new compounds in the crude herbal extract. This article described the metabolite profiling of ashwagandha root hydroalcoholic extract using ultra‐performance liquid chromatography coupled with a positive ion electrospray ionization tandem mass spectrometry through gas chromatography mass spectrometry (GC/MS) and NMR spectroscopy. A total of 43 possible withanolides was identified and proposed their structures based on the mass of molecular and fragment ions. GC/MS and NMR analysis indicated the presence of several known withanolides including withaferin A, withanolide D, withanoside IV or VI, withanolide sulfoxide, etc. To the best of our knowledge, dihydrowithanolide D at m/z 473 (tR 7.86 min) and ixocarpalactone A at m/z 505 (tR 8.43 min) were first time identified in the ashwagandha root hydroalcoholic extract. The current study that described the identification of withanolides with summarized literature review might be helpful for designing the experiment to identify of the new chemical constituents in Withania species.

Evaluation of the Physicochemical, Spectral, and Thermal Properties of Sodium Selenate Treated with the Energy of Consciousness (The Trivedi Effect®)

Sodium selenate is used for the prevention and treatment of various disorders like cancer, diabetes, inflammation diseases, etc. The objective of the current study was to evaluate the effect of The Trivedi Effect®-Energy of Consciousness Healing Treatment (Biofield Energy Treatment) on physicochemical, spectral, and thermal properties of The Trivedi Effect®-Biofield Energy Treated sodium selenate using various analytical methods such as PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Sodium selenate was divided into two parts – one part was control without any treatment and another part was treated with The Trivedi Effect®-Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as The Trivedi Effect®-Energy of Consciousness Healing Treated sample. The PXRD analysis showed the significant alteration of the crystallite size of the treated sample in the range of -36.36% to 133.24% as compared to the control sample. However, the average crystallite size of the treated sample was significantly decreased by 8.31% as compared to the control sample. Overall, the crystal morphology of the treated sample was different from the control sample. The particle sizes d10, d50, and d90 values of the treated sample were significantly decreased by 3.40%, 9.75%, and 8.52%, respectively as compared to the control sample. Consequently, the surface area of the treated sample was significantly increased by 5.26% from the control sample. Both the control and treated FT-IR spectra indicated the presence of sharp and strong absorption bands at 887 cm-1 due to the Se=O stretching. The UV-vis spectroscopic analysis displayed that the wavelength for the maximum absorbance of both the control and treated samples were at 204.1 and 204.0 nm, respectively. TGA analysis revealed that the total weight loss of the treated sample was reduced by 1.24% as compared with the control sample. The DSC analysis showed that the treated sample (588.77°C) had higher melting point than the control sample (588.60°C). The latent heat of fusion was reduced in the treated sample by 8.01% compared to the control sample. Thus, The Trivedi Effect®-Biofield Energy Healing might lead to generate a new polymorphic (enantiotropic) form of sodium selenate which would be more soluble, bioavailable, and thermally stable compared with the untreated sample. The Trivedi Effect treated sodium selenate would be very useful to design better nutraceutical and/or pharmaceutical formulations that might offer better therapeutic response against inflammatory diseases, immunological disorders, stress, aging, infectious diseases, cancer, diabetes, heart diseases, Alzheimer’s disease, etc.

Effect of the Energy of Consciousness (The Trivedi Effect®) on the Structural Properties and Isotopic Abundance Ratio of Magnesium Gluconate Using LC-MS and NMR Spectroscopy

Magnesium gluconate has the wide application for the prevention and treatment of hypomagnesemia. The objective of the current study was to investigate the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on magnesium gluconate for the change in the structural properties and isotopic abundance ratio (PM+1/PM) using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® - Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The LC-MS analysis of both the control and treated samples revealed the presence of the mass of the protonated magnesium gluconate at m/z 415 at the retention time of 1.53 minutes with almost similar fragmentation pattern. The relative peak intensities of the fragment ions of the treated sample were significantly altered compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were observed almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the percentage of the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) was significantly increased in treated sample by 80.38%, compared with the control sample. Briefly, 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416; 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated sample were significantly increased compared with the control sample. Thus, the treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. The Trivedi Effect® treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors using its kinetic isotope effects.

Characterization of Physical, Structural, Thermal, and Behavioral Properties of the Consciousness Healing Treated Zinc Chloride

Zinc chloride is an important pharmaceutical/nutraceutical compound used as a source of zinc. The objective of the current study was to investigate the impact of The Trivedi Effect®-Energy of Consciousness Healing Treatment (Biofield Energy Treatment) on physical, structural, thermal, and behavioral properties of zinc chloride using PXRD, PSD, FT-IR, UV-vis, and DSC analysis. Zinc chloride was divided into two parts – one part was control, while another part was treated with The Trivedi Effect® remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. A significant alteration of the crystallite size and relative intensities of the PXRD peaks was observed in The Trivedi Effect® treated sample compared with the control sample. A sharp peak at 2θ equal to 16.58° was observed in the control sample, but it was disappeared in the treated sample. The average crystallite size of the treated sample was significantly reduced by 21.31% compared with the control sample. The particle size values at d10, d50, and d90 were significantly decreased by 8.15%, 6.28%, and 5.26%, respectively in the treated sample compared with the control sample. The surface area of the treated sample was significantly increased by 7.14% compared with the control sample. The FT-IR spectroscopic analysis revealed that Zn-Cl stretching in the control and treated sample were found at 510 cm-1 and 511 cm-1, respectively. The UV-vis analysis exhibited that wavelength of the maximum absorbance (λmax) of both the control and treated samples was at 196 nm. The DSC analysis exhibited that the melting and decomposition temperature were decreased by 0.29% and 0.28%, respectively in the treated zinc chloride compared to the control sample. The latent heat of fusion of the treated sample (370.48 J/g) was increased significantly by 97.71% compared with the control sample (187.39 J/g). This results indicated that zinc chloride need more heat energy to undergo the process of melting after Biofield Energy Treatment. The enthalpy of decomposition of the treated sample was significantly decreased by 15.40% compared with the control sample. The current study anticipated that The Trivedi Effect®-Energy of Consciousness Healing Treatment might lead to produce a new polymorphic form of zinc chloride, which would be more soluble, bioavailable and latent heat of fusion compared with the untreated compound. Hence, the treated zinc chloride would be very useful to design better nutraceutical/pharmaceutical formulations that might offer better therapeutic response against inflammatory diseases, immunological disorders, aging, stress, cancer, etc.

Evaluation of Physicochemical, Spectral, Thermal and Behavioral Properties of the Biofield Energy Healing Treated Sodium Selenate

Sodium selenate is used for the prevention and treatment of cancer, diabetes, inflammatory diseases, etc. The objective of the current study was to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing treatment) on physical, structural, thermal, and behavioral properties of sodium selenate using PXRD, PSD, FT-IR, UV-vis, TGA, and DSC analysis. Sodium selenate was divided into two parts – one part was control, while another part was treated with The Trivedi Effect® remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® Treated sample. The PXRD analysis showed the significant alteration of the crystallite size of the treated sample in the range of -49.99% to 50.01% compared to the control sample. However, the average crystallite size of the treated sample was decreased by 0.72% compared with the control sample. Overall, the crystal morphology of the treated sample was different from the control sample. The particle size of the treated sample at d10, d50, and d90 values were significantly decreased by 3.71%, 6.72%, and 26.17%, respectively compared with the control sample. Consequently, the surface area of the treated sample was significantly increased by 6% from the control sample. The control and treated FT-IR spectra indicated the presence of sharp and strong absorption bands at 886 cm-1 and 887 cm-1, respectively due to the Se=O stretching. The UV-vis spectroscopic analysis displayed that the wavelength for the maximum absorbance of the control and treated samples were at 204.8 and 204.9 nm, respectively. TGA analysis revealed that the total weight loss of the treated sample was reduced by 4.93% compared with the control sample. The DSC analysis showed that the treated sample (95.10°C) had higher vaporization temperature than the control sample (94.80°C). The latent heat of vaporization was increased significantly in the treated sample by 14.68% compared to the control sample. Thus, The Trivedi Effect® - Energy of Consciousness Healing Treatment might lead to generate a new polymorphic form of sodium selenate which would be more soluble, bioavailable, and thermally stable compared with the untreated sample. The Trivedi Effect® treated sodium selenate would be very useful to design better nutraceutical and/or pharmaceutical formulations that might offer better therapeutic response against inflammatory diseases, immunological disorders, stress, aging, infectious diseases, cancer, diabetes, heart diseases, Alzheimer’s disease, etc.

Solid State Characterization of Withania somnifera (Ashwagandha) Root Extract After Treatment with Consciousness Energy Healing

Ashwagandha root extract is useful as an herbal medicine and nutraceuticals for the prevention and treatment of various diseases. The aim of the current study was to evaluate the influence of Consciousness Energy Healing Treatment (The Trivedi Effect®) on the physico-chemical, thermal and behavioral properties of ashwagandha root extract using powder X-ray diffraction (PXRD), particle size distribution analysis (PSD), Fourier transform infrared (FT-IR) spectrometry, UV-Vis spectroscopy, thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). Ashwagandha root extract was divided into two parts – one part was control without any Biofield Energy Treatment, and another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as Biofield Energy Treated sample. The PXRD analysis concluded that both the control and treated samples were amorphous in nature. The particle size values at d10, d50, and d90 of the treated sample were significantly decreased by 36.78%, 15.18%, and 5.06%, respectively compared with the control sample. Likewise, the surface area of the treated sample was significantly increased by 85.14% compared to the control sample. FT-IR results showed a small impact of Consciousness Energy Healing Treatment on the phytoconstituents of ashwagandha root extract to reduce the force constant of O-H (str.) bond. UV-vis analysis revealed that the wavelength for the maximum absorbance (λmax) of both the samples was at 206.4 in methanol. TGA revealed the three steps of thermal degradation and the total weight loss was decreased by 0.73% in the treated sample compared to the control sample. Consequently, the maximum thermal degradation temperature was found at 272.53°C and 393.35°C for two broad peaks in the treated sample was increased by 0.05% and 0.08%, respectively compared to the control the sample (272.67°C and 393.66°C). The DSC analysis indicated that the evaporation temperature and latent heat of vaporization were lowered significantly by 4.98% and 35.67%, respectively in the treated sample compared with the control sample. The current outcomes suggested that the Energy of Consciousness Healing Treatment might have the amazing capacity to enhance the solubility, dissolution, absorption, bioavailability and thermal stability of ashwagandha root extract in the various form of pharmaceutical and nutraceutical formulation by modifying its particle size and surface area. Thus, the Biofield Energy Treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, sexual disorders, arthritis, stress, cancer, ageing, diabetes, and other chronic infections.

Study of the Energy of Consciousness Healing Treatment on Physical, Structural, Thermal, and Behavioral Properties of Zinc Chloride

Zinc chloride is an important pharmaceutical/nutraceutical mineral for the prevention and treatment of several diseases. The objective of the current study was to investigate the impact of The Trivedi Effect®-Energy of Consciousness Healing Treatment (Biofield Energy Treatment) on physical, structural, thermal, and behavioral properties of zinc chloride using PXRD, PSD, FT-IR, UV-vis, and DSC analysis. Zinc chloride was divided into two parts – one part was control, while another part was treated with The Trivedi Effect® remotely by seven renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. A significant alteration of the crystallite size and relative intensities of the PXRD peaks were observed in The Trivedi Effect® treated sample with respect to the control sample. A sharp peak at 2θ equal to 51.45°, which was observed in the control sample was disappeared in the treated sample. The average crystallite size of the treated sample was decreased by 2.13% compared with the control sample. The size of the particles at d10, d50, and d90 was decreased by 3.36%, 7.97%, and 11.51%, respectively in the treated sample compared with the control sample. The surface area of the treated sample was significantly increased by 5.69% compared with the control sample. The FT-IR spectroscopic analysis revealed that Zn-Cl stretching in the control was 646 cm-1, whereas it was significantly shifted downward to 505 cm-1 in the treated sample. The UV-vis analysis exhibited that wavelength of the maximum absorbance (λmax) of both the control and treated samples was at 196.4 nm. The DSC analysis exhibited that the melting and decomposition temperature were increased by 0.93% and 0.56%, respectively in the treated zinc chloride compared to the control sample. The enthalpy of fusion of the treated sample (338.27 J/g) was increased significantly by 33.84% compared with the control sample (252.75 J/g). The enthalpy of decomposition of the treated sample was increased by 3.78% compared with the control sample. The current study anticipated that The Trivedi Effect®-Energy of Consciousness Healing Treatment might lead to produce a new polymorphic form of zinc chloride, which would be more soluble, bioavailable, and thermally stable compared with the untreated compound. The treated sample could be more stable during manufacturing, delivery or storage conditions than the untreated sample. Hence, the treated zinc chloride would be very useful to design better nutraceutical/pharmaceutical formulations that might offer better therapeutic response against inflammatory diseases, immunological disorders, aging, stress, cancer, etc.

Liquid Chromatography Tandem Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy of Magnesium (II) Gluconate Solution

Magnesium gluconate is a classical pharmaceutical compound used as a source of magnesium for the prevention and treatment of hypomagnesemia. To the best of our knowledge, a robust and reliable liquid chromatography tandem mass spectrometry technique has not yet been reported for the qualitative and quantitative analysis of magnesium gluconate. This study describes the method development for the LC–ESI–MS/MS analysis of magnesium gluconate using three different reversed-phase HPLC conditions (Method I–III) with comprehensive fragmentation pattern and the structural characterization by NMR spectroscopy. The LC–MS and NMR data were found in accordance with the structure of magnesium gluconate. When magnesium gluconate was dissolved in the acetonitrile and water–methanol solutions, it exists in situ in three different forms: magnesium gluconate itself, gluconic acid, and magnesium gluconate chelate with gluconic acid by a coordinate covalent bond. Method I exhibited pseudo-molecular ion peaks with more magnesium gluconate chelates with gluconic acid, while method II showed an adduct of magnesium gluconate with the solvent along with the molecular ion peak. There was no pseudo-molecular ion peaks found in method III. Thus, method III was found to be the more accurate, robust and reliable LC–MS method for the qualitative and quantitative analysis, structural characterization, and could also be suitable for the pharmacokinetic study of magnesium gluconate. The detailed fragmentation analysis might be useful for the structural characterization of unknown divalent organometallics.