Patricia Farnes

HUMAN MAMMARY SLICES IN ORGAN CULTURE. I. METHOD OF CULTURE AND PRELIMINARY OBSERVATIONS ON THE EFFECT OF INSULIN.

Abstract A technique for culture of human mammary slices has been devised, which permits survival and limited proliferation of all elements for periods up to 12 days in vitro. Slices survive in a variety of media with or without serum, providing that glucose concentrations are maintained above critical levels. In these organ cultures, addition of insulin to the medium results in marked morphological changes of ductal epithelium including proliferation, hypertrophy, and foci resembling squamous metaplasia.

Cytochemical studies of human bone marrow fibroblast-like cells. I. Alkaline phosphatase.

Abstract Alkaline phosphatase activity of marrow fleck components and fibroblast-like cells (FLC) developing in marrow cultures has been compared. The enzyme is demonstrable in the vascular network of marrow stroma, maturing myeloid cells, and FLC developing in culture. The vascular network of marrow flecks visualized by this method is described. It is concluded that the FLC originate from marrow stroma and not from any hemic cells, in this culture system. The most likely parent cell of the FLC is the endothelial cell.

Tissue culture studies of human bone marrow: II. Protein synthesis in haemic cells

Abstract Human bone marrow cells cultivated in a chemically defined system incorporate tritiated histidine, phenylalanine, or leucine in vitro, as demonstrated by autoradiographic studies. Megakaryocytes show intense labelling up to 3 days, and platelet formation also occurs. Immature members of the granulocytic series, both neutrophilic and eosinophilic, show heavy labelling. Promyelocytes show greatest amino acid incorporation, and mature neutrophiles and eosinophiles show the least. Erythroblasts label in early cultures, and occasional erythrocytes, probably reticulocytes, also show significant incorporation. The presence of prednisolone in the medium is associated with increased incorporation of precursor as compared to that of cells cultured in hormone-free medium.

A technique for chromosome study of human bone marrow fibroblast-like cells☆

Abstract 1. 1. A technique for chromosome analysis of fibroblast-like cells from human bone marrow tissue cultures is presented. 2. 2. This technique may also be applied to the study of skin fibroblasts from skin explants.