Patricia Vieyra-Reyes

Limbic system pathologies associated with deficiencies and excesses of the trace elements iron, zinc, copper, and selenium

Deficiencies of nutrients such as amino acids, vitamins, lipids, and trace elements during gestation and early infanthood have strong deleterious effects on the development of the limbic system; these effects may be irreversible, even when adequate supplementation is provided at later developmental stages. Recent advances in the neurochemistry of biometals are increasingly establishing the roles of the trace elements iron, copper, zinc, and selenium in a variety of cell functions and are providing insight into the repercussions of deficiencies and excesses of these elements on the development of the central nervous system, especially the limbic system. The limbic system comprises diverse areas with high metabolic demands and differential storage of iron, copper, zinc, and selenium. This review summarizes available evidence suggesting the involvement of these trace elements in pathological disorders of the limbic system.

Lipopolysaccharide Binding Protein and Oxidative Stress in a Multiple Sclerosis Model

Recent findings in experimental autoimmune encephalomyelitis (EAE) suggest that altering certain bacterial populations present in the gut may lead to a proinflammatory condition, that could result in the development of multiple sclerosis (MS). Also, Reactive Oxygen Species seem to be involved in the course of MS. In this study, it has been aimed to relate all these variables starting from an analysis of the lipopolysaccharide (LPS) and LPS-binding protein (LBP) with the determination of parameters related to oxidative stress in the blood, brain and spinal cord. For this purpose, samples obtained from EAE rats and relapsing-remitting (RRMS) MS patients were used. In addition, EAE rats were treated with Natalizumab, N-acetyl-cysteine and dimethyl fumarate. Natalizumab was also employed in RRMS. The results of this study revealed an improvement in the clinical symptoms of the EAE and MS with the treatments, as well as a reduction in the oxidative stress parameters and in LBP. Correlations between the clinical variables of the disease, i.e. oxidative damage and LBP, were established. Although the conclusions of this research are indeed relevant, further investigation would be necessary to establish the intrinsic mechanisms of the MS-oxidative stress-microbiota relationship.

An iron-deficient diet during development induces oxidative stress in relation to age and gender in Wistar rats

Iron is a trace element and a structural part of antioxidant enzymes, and its requirements vary according to age and gender. We hypothesized that iron deficiency (ID) leads to an increase in free radicals which mainly affect the brain, and the severity of damage would therefore be dependent on age and gender. Two groups of Wistar rats were evaluated evolutionarily: 100 rats (50 males; 50 females) with ID diet and 100 rats (50 males; 50 females) with standard diet. Both groups were offspring from mothers who were previously under the same dietary intervention. The ages studied roughly correspond to stages of human development: birth (0 postnatal day “PND” in rats), childhood (21 PND), early adolescence (42 PND), late adolescence (56 PND), and adulthood (70 PND). The following biomarkers in the brain, blood, and liver were analyzed: lipid peroxidation products (LPO), protein carbonyl content and activity of the antioxidant enzymes, superoxide dismutase, catalase, and glutathione peroxidase. It was demonstrated that ID subjects are born with high levels of LPO in the brain and low antioxidant activity, the damage being more severe in males. After birth, antioxidant defense focuses on the central level (brain) in ID females and on the peripheral level (blood and liver) in ID males. In two critical stages of development, birth and late adolescence, antioxidant protection is insufficient to counteract oxidative damage in ID subjects. Moreover, we observed that the variability of results in the literature on oxidative stress and ID comes from gender and age of the subjects under study. With this, we can establish patterns and exact moments to carry out studies or treatments.

Disfunción familiar y depresión en niños de 8-12 años de edad

La poblacion estudiada incluyo 326 ninos, de 8 a 12 anos de edad. De ellos el 54.3% correspondio al genero femenino y el 45.7% al masculino, predominando en frecuencia los ninos en edades de 9 a 11 anos.

New track-structure Monte Carlo code for 4D ionizing photon transport

ABSTRACT Radiodiagnosis and radiation treatment, in each of their areas such as imaging, radiotherapy and nuclear medicine, require precise calculations about the energy deposited and scattering of the ionizing radiation used. In healthcare applications, it is required to know the penetration and amount of energy deposited in the biological tissue irradiated by ionizing photons; these parameters are function of the photon interaction processes with matter, which can be analyzed experimentally or by Monte Carlo simulation. Purpose: The aim of this work was to develop a new Monte Carlo code for ionizing photon transport in water with the track structure technique, that allows to discriminate primary and secondary photons, and to determine the energy deposited, interaction coordinates, path length and time of flight (TOF) inside of scatter volume. Methods: C++ programming language was used. In the Compton scattering, the polar angle was sampled by methods: Kahn and EGS. Water spheres centered at the origin with different radius were used, where the isotropic point source was placed at (0, 0, 0) for different energies to compute the energy lost by photons and TOF inside spherical volume. Results: It was determined that the best sampling method for the polar angle generation in each Compton interaction was the EGS method. Energy deposited in target region filled with water was compared with MCNPX 2.6 and others’ results from literature. Mean TOF and pathlength inside region of interest was obtained for 4 radii and 5 energies. Conclusions: Quantities computed with the new code are, according to reported data, and so, the new code is reliable for photon transport in water using the track structure method; this will allow the new code to become a useful tool in the areas of radiology and radiation dosimetry. Also, TOF inside scatter volume was reported.

67Ga as a biosensor of iron needs in different organs: Study performed on male and female rats subjected to iron deficiency and exercise

The aim of the present study was to determine the iron needs in different organs and tissues using 67Ga as a biosensor in males and females rats subjected to iron deficiency (ID) and voluntary exercise (EX). 67Ga citrate was injected i.p. to female and male Wistar rats (n=5/sex/group). Groups: Control (sedentary conditions), Control+EX, ID and ID+EX. To determine the 67Ga uptake, samples from the following regions of interest (ROIs) were extracted 12h post-injection: blood, liver, gonads, bone marrow, heart, adrenal glands, skeletal muscle, stomach, kidney, eyeball, sciatic nerve, small intestine and peritoneum. The total 67Ga uptake was 412% higher in ID subjects than in control subjects, being 1011% higher in ID-males than ID-females. In ID-females, the ROIs with the greater 67Ga uptake were blood, kidney and bone marrow, while in ID-males they were sciatic nerve, eyeball and adrenals, which demonstrates that the biodistribution differed between sexes in sedentary conditions but when subjected to EX, the biodistribution was similar in each sex group although females had a greater 67Ga uptake. In ID+EX subjects, the ROIs that showed the highest uptake were sciatic nerve, eyeball and adrenal glands. Using 67Ga as a biosensor, it is possible to identify the needs of iron that each organ requires to perform their functions in normal physiological conditions. In addition, a higher or lower 67Ga uptake in a specific organ may indicate its malfunction or show damage.