Patrick Stanssens
Ablynx
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Publication
Featured researches published by Patrick Stanssens.
Blood | 2011
Hans Ulrichts; Karen Silence; Anne Schoolmeester; Peter de Jaegere; Stefaan Rossenu; Jan Roodt; Sofie Priem; Marc Lauwereys; Peter Casteels; Femke van Bockstaele; Katrien Verschueren; Patrick Stanssens; Judith Baumeister; Josefin Beate Holz
Neutralizing the interaction of the platelet receptor gpIb with VWF is an attractive strategy to treat and prevent thrombotic complications. ALX-0081 is a bivalent Nanobody which specifically targets the gpIb-binding site of VWF and interacts avidly with VWF. Nanobodies are therapeutic proteins derived from naturally occurring heavy-chain-only Abs and combine a small molecular size with a high inherent stability. ALX-0081 exerts potent activity in vitro and in vivo. Perfusion experiments with blood from patients with acute coronary syndrome on standard antithrombotics demonstrated complete inhibition of platelet adhesion after addition of ALX-0081, while in the absence of ALX-0081 residual adhesion was observed. In a baboon efficacy and safety model measuring acute thrombosis and surgical bleeding, ALX-0081 showed a superior therapeutic window compared with marketed antithrombotics. Pharmacokinetic and biodistribution experiments demonstrated target-mediated clearance of ALX-0081, which leads to a self-regulating disposition behavior. In conclusion, these preclinical data demonstrate that ALX-0081 combines a high efficacy with an improved safety profile compared with currently marketed antithrombotics. ALX-0081 has entered clinical development.
Microbial Cell Factories | 2016
Peter Schotte; Isabelle Dewerte; Manu De Groeve; Saskia De Keyser; Veronique De Brabandere; Patrick Stanssens
BackgroundOver the last few decades the methylotrophic yeast Pichia pastoris has become a popular host for a wide range of products such as vaccines and therapeutic proteins. Several P. pastoris engineered strains and mutants have been developed to improve the performance of the expression system. Yield and quality of a recombinant product are important parameters to monitor during the host selection and development process but little information is published regarding quality differences of a product produced by different P. pastoris strains.ResultsWe compared titer and quality of several Nanobodies® produced in wild type and MutS strains. Titer in fed-batch fermentation was comparable between all strains for each Nanobody but a significant difference in quality was observed. Nanobodies expressed in MutS strains contained a product variant with a Δ−16xa0Da mass difference that was not observed in wild type strains. This variant showed substitution of methionine residues due to misincorporation of O-methyl-l-homoserine, also called methoxine. Methoxine is likely synthesized by the enzymatic action of O-acetyl homoserine sulfhydrylase and we confirmed that Nanobodies produced in the corresponding knock-out strain contained no methoxine variants. We could show the incorporation of methoxine during biosynthesis by its addition to the culture medium.ConclusionWe showed that misincorporation of methoxine occurs particularly in P. pastoris MutS strains. This reduction in product quality could outweigh the advantages of using Mut strains, such as lower oxygen and methanol demand, heat formation and in some cases improved expression. Methoxine incorporation in recombinant proteins is likely to occur when an excess of methanol is present during fermentation but can be avoided when the methanol feed rate protocol is carefully designed.
Archive | 1999
George P. Vlasuk; Patrick Stanssens; Joris Hilda Lieven Messens; Marc Lauwereys; Yves Laroche; Laurent Jespers; Yannick Gansemans; Matthew Moyle; Peter W. Bergum
Archive | 1995
George P. Vlasuk; Patrick Stanssens; Joris Hilda Lieven Messens; Marc Lauwereys; Yves Laroche; Laurent Jespers; Yannick George Jozef Gansemans; Matthew Moyle; Peter W. Bergum
Archive | 2009
Marc Zabeau; Patrick Stanssens; Yannick Gansemans
Archive | 2009
Jean-Luc Jonniaux; Marc Lauwereys; Patrick Stanssens
Archive | 2010
Peter Schotte; Patrick Stanssens; Christine Labeur; Jean-Luc Jonniaux; Marc Lauwereys
Archive | 2009
Peter Casteels; Marc Lauwereys; Patrick Stanssens; Christine Labeur; Carlo Boutton; Anne Brigé; Hendricus Renerus Jacobus Mattheus Hoogenboom; Els Anna Alice Beinaert
Archive | 2004
Marc Zabeau; Patrick Stanssens; Yannick Gansemans
Archive | 2000
Wim Quax; Yves Laroche; Adrianus Wilhelmus Vollebregt; Patrick Stanssens; Marc Lauwereys