Paul R. Sheppard

Temporal Variability of Tungsten and Cobalt in Fallon, Nevada

Background Since 1997, Fallon, Nevada, has experienced a cluster of childhood leukemia that has been declared “one of the most unique clusters of childhood cancer ever reported.” Multiple environmental studies have shown airborne tungsten and cobalt to be elevated within Fallon, but the question remains: Have these metals changed through time in correspondence with the onset of the leukemia cluster? Methods We used dendrochemistry, the study of element concentrations through time in tree rings, in Fallon to assess temporal variability of airborne tungsten and cobalt since the late 1980s. The techniques used in Fallon were also tested in a different town (Sweet Home, OR) that has airborne tungsten from a known source. Results The Sweet Home test case confirms the accuracy of dendrochemistry for showing temporal variability of environmental tungsten. Given that dendrochemistry works for tungsten, tree-ring chemistry shows that tungsten increased in Fallon relative to nearby comparison towns beginning by the mid-1990s, slightly before the onset of the cluster, and cobalt has been high throughout the last ~ 15 years. Other metals do not show trends through time in Fallon. Discussion Results in Fallon suggest a temporal correspondence between the onset of excessive childhood leukemia and elevated levels of tungsten and cobalt. Although environmental data alone cannot directly link childhood leukemia with exposure to metals, research by others has shown that combined exposure to tungsten and cobalt can be carcinogenic to humans. Conclusion Continued biomedical research is warranted to directly test for linkage between childhood leukemia and tungsten and cobalt.

A 700-year history of groundwater recharge in the drylands of NW China

A 700-year semi-quantitative history of diffuse groundwater recharge in the Badain Jaran Desert (Inner Mongolia) is proposed on the basis of solute data from multiple unsaturated zone groundwater profiles using mass balance of chloride to establish recharge rates and profile chronologies. Four relatively humid (1330—1430, 1500—1620, 1700—1780 and 1950—1990) and three relatively arid phases (1430—1500, 1620—1700 and 1900—1950) are discernable across the profiles. The recharge history broadly reflects multidecadal to centurial timescale precipitation changes in the northern Tibetan Plateau and suggests that variations in East Asian Summer Monsoon intensity affect desert recharge rates. Uncertainties in the records owing to assumptions about the Cl inputs are examined by comparing deterministic and stochastic Cl input scenarios. Such records are valuable for assessing spatial aspects of climate changes in the region, as well as for informing sustainable water resource management strategies for northwestern Chinas drylands.

Reflected-light image analysis of conifer tree rings for reconstructing climate

We use reflected-light image analysis to measure brightness of conifer rings, and we use brightness as an alternative to density for reconstructing climate. Using densitometry and image analysis, we measured cores from red spruce (Picea rubens Sarg.) growing at Elephant Mountain, Maine, and then compared statistical characteristics of density and brightness and climate-tree growth models using density or brightness. Auto- correlational and cross-correlational statistics of density and brightness do not differ substantially, and late wood density and brightness both correlate with April-May average temperature, which both tree-ring variables reconstruct equally well. April-May temperature was highly variable during most of the nineteenth century and was below average during the AD 1830s, 1870s and late 1880s. When done carefully, reflected-light image analysis can substitute for X-ray densitometry for measuring tree rings to reconstruct climate of the latest Holo cene.

Application of tree-ring analysis to paleoseismology: Two case studies

Knowledge of earthquake probabilities is essential for planning earthquake hazard mitigation, and valid estimation of future probabilities requires precise information on past earthquake occurrences. Previous studies documented effects of earthquakes on trees and how these effects are recorded by tree rings. Tree-ring analysis can be combined with other disciplines to date and delineate earthquake induced disturbance. Two case studies show the impact of the 1964 Alaska earthquake on shoreline trees and how a previously unknown southern San Andreas fault earthquake was recorded in tree rings.

Multiple Dendrochronological Signals Indicate The Eruption Of Parícutin Volcano, Michoacán, Mexico

Abstract The eruption of Parícutin (1943–1952), a cinder cone volcano in Michoacán, Mexico, caused dendrochronological and dendrochemical responses that might be useful as general dating tools for eruptions. For the eruption period, pines near Parícutin have slightly suppressed ring widths plus high inter-annual variability of width. Wood anatomy changes include traumatic resin ducts and thin bands of false latewood. Dendrochemistry of tree rings shows little temporal variation in most elements, but beginning in 1943 sulfur content increased in rings of four trees and phosphorus content increased in rings of two trees. Hypotheses for increased S and P include new availability of pre-existing soil S and P and/or new input of S and P from the tephra itself. Pines at Parícutin also show suppressed ring widths for five years beginning in 1970, and had the eruption date not been known, the most likely conclusion from ring-width data alone would have been an eruption from 1970 to 1974. However, the 1970s suppression was in response to defoliation by a pine sawfly outbreak, not an eruption. For dendrochronological dating of cinder-cone eruptions, a combination of multiple characteristics (width, chemistry, and anatomy) would be more reliable than depending on any one characteristic alone.

Strategies for evaluating the environment–public health interaction of long-term latency disease: The quandary of the inconclusive case–control study

Environmental links to disease are difficult to uncover because environmental exposures are variable in time and space, contaminants occur in complex mixtures, and many diseases have a long time delay between exposure and onset. Furthermore, individuals in a population have different activity patterns (e.g., hobbies, jobs, and interests), and different genetic susceptibilities to disease. As such, there are many potential confounding factors to obscure the reasons that one individual gets sick and another remains healthy. An important method for deducing environmental associations with disease outbreak is the retrospective case-control study wherein the affected and control subject cohorts are studied to see what is different about their previous exposure history. Despite success with infectious diseases (e.g., food poisoning, and flu), case-control studies of cancer clusters rarely have an unambiguous outcome. This is attributed to the complexity of disease progression and the long-term latency between exposure and disease onset. In this article, we consider strategies for investigating cancer clusters and make some observations for improving statistical power through broader non-parametric approaches wherein sub-populations (i.e., whole towns), rather than individuals, are treated as the cases and controls, and the associated cancer rates are treated as the dependent variable. We subsequently present some ecological data for tungsten and cobalt from studies by University of Arizona researchers who document elevated levels of tungsten and cobalt in Fallon, NV. These results serve as candidates for future hybrid ecologic case-control investigations of childhood leukemia clusters.

Dose-dependent transcriptome changes by metal ores on a human acute lymphoblastic leukemia cell line

The increased morbidity of childhood leukemia in Fallon, Nevada and Sierra Vista, Arizona has prompted great health concern. The main characteristic that these two towns share is the environmental pollution attributed to metal ore from abandoned mining operations. Consequently, we have investigated the transcriptome effects of metal ores from these endemic areas using a human T-cell acute lymphoblastic leukemia cell line (T-ALL). Metal ore from Fallon significantly increased cell growth after 24, 48 and 72 h of incubation at 1.5 mg/mL concentration, as measured by trypan-blue. Sierra Vista ore significantly increased cell growth with 0.15 and 1.5 mg/mL following 72 h of incubation. From human cDNA microarray, results indicate that in total, eight genes, mostly metallothionein (MT) genes, were up-regulated and 10 genes were down-regulated following treatment of the T-ALL cells with 0.15 and 1.5 mg/mL of metal ores at 72 h, in comparison with untreated cells. Twenty-eight metals of both ores were quantified and their presence may be associated with the cell growth rate and dose-dependent activation of transcriptomes in immature T-cells.

Streamflow variability in the Chilean Temperate-Mediterranean climate transition (35°S–42°S) during the last 400 years inferred from tree-ring records

Abstract As rainfall in South-Central Chile has decreased in recent decades, local communities and industries have developed an understandable concern about their threatened water supply. Reconstructing streamflows from tree-ring data has been recognized as a useful paleoclimatic tool in providing long-term perspectives on the temporal characteristics of hydroclimate systems. Multi-century long streamflow reconstructions can be compared to relatively short instrumental observations in order to analyze the frequency of low and high water availability through time. In this work, we have developed a Biobío River streamflow reconstruction to explore the long-term hydroclimate variability at the confluence of the Mediterranean-subtropical and the Temperate-humid climate zones, two regions represented by previous reconstructions of the Maule and Puelo Rivers, respectively. In a suite of analyses, the Biobío River reconstruction proves to be more similar to the Puelo River than the Maule River, despite its closer geographic proximity to the latter. This finding corroborates other studies with instrumental data that identify 37.5°S as a latitudinal confluence of two climate zones. The analyzed rivers are affected by climate forcings on interannual and interdecadal time-scales, Tropical (El Niño Southern Oscillation) and Antarctic (Southern Annular Mode; SAM). Longer cycles found, around 80-years, are well correlated only with SAM variation, which explains most of the variance in the Biobío and Puelo rivers. This cycle also has been attributed to orbital forcing by other authors. All three rivers showed an increase in the frequency of extreme high and low flow events in the twentieth century. The most extreme dry and wet years in the instrumental record (1943–2000) were not the most extreme of the past 400-years reconstructed for the three rivers (1600–2000), yet both instrumental record years did rank in the five most extreme of the streamflow reconstructions as a whole. These findings suggest a high level of natural variability in the hydro-climatic conditions of the region, where extremes characterized the twentieth century. This information is particularly useful when evaluating and improving a wide variety of water management models that apply to water resources that are sensitive to agricultural and hydropower industries.

Exposure to sodium tungstate and Respiratory Syncytial Virus results in hematological/immunological disease in C57BL/6J mice

The etiology of childhood leukemia is not known. Strong evidence indicates that precursor B-cell Acute Lymphoblastic Leukemia (Pre-B ALL) is a genetic disease originating in utero. Environmental exposures in two concurrent, childhood leukemia clusters have been profiled and compared with geographically similar control communities. The unique exposures, shared in common by the leukemia clusters, have been modeled in C57BL/6 mice utilizing prenatal exposures. This previous investigation has suggested in utero exposure to sodium tungstate (Na2WO4) may result in hematological/immunological disease through genes associated with viral defense. The working hypothesis is (1) in addition to spontaneously and/or chemically generated genetic lesions forming pre-leukemic clones, in utero exposure to Na2WO4 increases genetic susceptibility to viral influence(s); (2) postnatal exposure to a virus possessing the 1FXXKXFXXA/V9 peptide motif will cause an unnatural immune response encouraging proliferation in the B-cell precursor compartment. This study reports the results of exposing C57BL/6J mice to Na2WO4 in utero via water (15 ppm, ad libetum) and inhalation (mean concentration PM5 3.33 mg/m3) and to Respiratory Syncytial Virus (RSV) within 2 weeks of weaning. Inoculation of C57BL/6J mice with RSV was associated with a neutrophil shift in 56% of 5-month old mice. When the RSV inoculation was combined with Na2WO4-exposure, significant splenomegaly resulted (p=0.0406, 0.0184, 0.0108 for control, Na2WO4-only and RSV-only, respectively) in addition to other hematological pathologies which were not significant. Exposure to Na2WO4 and RSV resulted in hematological/immunological disease, the nature of which is currently inconclusive. Further research is needed to characterize this potential leukemia mouse model.

Tree-ring responses to the 1978 earthquake at Stephens Pass, northeastern California

The 1978 earthquake at Stephens Pass, northeastern California, dropped a series of grabens that average 4.5 m in width, extend up to 1 m in depth, and are found intermittently along a 2-km-long rupture zone. The formation of this graben series killed or otherwise affected many trees growing in or immediately adjacent to the rupture zone. Nine trees responded to the 1978 earthquake with anomalously narrow ring widths, beginning in 1979 and continuing for several years. One tree responded with anomalously wide latewood relative to total ring width. This example of tree-ring responses to a normal-fault earthquake complements other cases of tree-growth responses to earthquakes of thrust and strike-slip tectonic settings. The 1978 earthquake at Stephens Pass was unique in that it caused tree-ring responses even though it was only moderate (magnitude 4.6). This study serves as a specific calibration example for dendrochronologically studying prehistoric earthquakes, as well as eruptions, at the nearby Medicine Lake Highlands. Medicine Lake has been seismically and volcanically active during the past 1000 yr, and it supports a forest of several coniferous tree species that can be used for dendrochronologically studying geomorphological processes.