Paul Williams

Human monocyte isolation methods influence cytokine production from in vitro generated dendritic cells

There is growing interest in the in vitro generation of dendritic cells (DC) from peripheral blood monocytes, but the effect of the method chosen to isolate CD14+ monocytes for subsequent DC generation is poorly documented. The method used to isolate monocytes may have an impact on the subsequent function of DC by affecting their ability to express costimulatory molecules (CD80/86), maturation marker (CD83) and/or to produce important immunomodulatory cytokines. In this study, we show that the positive selection of monocytes by anti‐CD14‐coated microbeads inhibits the lipopolysaccharide (LPS)‐induced production of interleukin (IL)‐12, IL‐10 and tumour necrosis factor‐α (TNF‐α) from human DC. However, when DC were grown from monocytes isolated by plastic adherence, LPS induced the production of much higher levels of these cytokines. DC derived from adherence‐isolated monocytes induced the development of potent cytotoxic T lymphocytes of the Tc1 subset specific for influenza matrix protein, as confirmed by interferon‐γ (IFN‐γ) enzyme‐linked immunosorbent spot‐forming cell assay (ELISPOT), cytotoxicity assay, major histocompatibility complex (MHC)–peptide tetrameric complexes and T helper 1/T helper 2 (Th1/Th2) cytokine production assays.

Transcranial magnetic stimulation: Improved coil design for deep brain investigation

This paper reports on a design for a coil for transcranial magnetic stimulation. The design shows potential for improving the penetration depth of the magnetic field, allowing stimulation of subcortical structures within the brain. The magnetic and induced electric fields in the human head have been calculated with finite element electromagnetic modeling software and compared with empirical measurements. Results show that the coil design used gives improved penetration depth, but also indicates the likelihood of stimulation of additional tissue resulting from the spatial distribution of the magnetic field.

Study of the magnetite to maghemite transition using microwave permittivity and permeability measurements

The microwave cavity perturbation (MCP) technique is used to identify the transition from magnetite (Fe3O4) to the meta-stable form of maghemite (γ-Fe2O3). In this study Fe3O4 was annealed at temperatures from 60 to 300 °C to vary the oxidation. Subsequent to annealing, the complex permittivity and magnetic permeability of the iron oxide powders were measured. The transition to γ-Fe2O3 was corroborated with x-ray diffraction (XRD), x-ray photoelectron spectroscopy (XPS) and vibrating sample magnetometry (VSM). XRD, XPS and VSM implied that the starting powder was consistent with Fe3O4 and the powders annealed at more than 200 °C were transitioning to γ-Fe2O3. The MCP measurements gave large differences in both complex permittivity and magnetic permeability of the two phases in the frequency range of 2.5-10.2 GHz. Magnetic permeability decreased with annealing temperature, though magnetic losses showed frequency dependent behaviour. Complex permittivity measurements showed a large decrease in both dielectric constant and losses at all measurement frequencies, as well as a prominent loss peak centred around the phase transition temperatures. We interpret the loss peak as being a consequence of field effects due to an intermediate multi-phase mixture. Additionally, almost no frequency dependence was observed. The reduction in complex permittivity implies that the Feoct(2+) cations in the lattice provide a significant contribution to polarization at microwave frequencies and the effects of Feoct(3+) are nominal in comparison.. The change in loss can be explained as a combination of the differences in the effective conductivity of the two phases (i.e. Fe3O4 exhibits electron-hopping conduction whereas the presence of vacancies in γ-Fe2O3 nullifies this). This shows that the non-invasive MCP measurements serve as a highly sensitive and versatile method for looking at this phase transition in iron and potentially the effects of oxidation states on the polarization in other iron oxides.

Design and evaluation of a 3D printed optical sensor for monitoring finger flexion

The development of techniques for monitoring finger movement is becoming increasingly important in areas, such as robotics, virtual reality, and rehabilitation. To date, various techniques have been proposed for tracking hand movements, but the majority suffer from poor accuracy and repeatability. Inspired by the articulated structure of finger joints, we propose a novel 3-D printed optical sensor with a compact hinged configuration for tracking finger flexion. This sensor exploits Malus’ law using the attenuation of light transmitted through crossed polarizers. The sensor consists of a single LED, two pieces of linear polarizing film, and a photodetector that detects the changes in polarized light intensity proportional to the angle of finger flexion. This paper presents the characterization of the proposed optical sensor and compares it with a commonly used commercial bend sensor. Results show that the bend sensor exhibits hysteresis error, low sensitivity at small angles, and significant temporal drift. In contrast, the optical sensor is more accurate (±0.5°) in the measuring range from 0° to 90°, and exhibits high repeatability and stability, as well as a fast dynamic response. Overall, the optical sensor outperforms the commercial bend sensor, and shows excellent potential for monitoring hand movements in real time.

A Novel Magnetostrictive Curvature Sensor Employing Flexible, Figure-of-Eight Sensing Coils

The demand for accurate angle measurements in a form that is robust and small is high, due to the advances in virtual reality applications, primarily the virtual reality headsets. The peripheral devices are required to completely immerse the user in a virtual reality setting, and for this purpose, a robust sensor has been developed. The angle measurements can also be used in motion sensing applications for medical purposes, allowing monitoring of a patients condition. This paper presents the development of a planar figure-of-eight coil sensor, which has been designed for the purpose of curvature sensing. The copper-plated polyimide material, FR4 FLEX, was used for the fabrication of the planar figure-of-eight coil. A curvature sensor was designed and consists of the figure-of-eight coil along with the magnetostrictive material Metglas 2605SA1. The sensor was incorporated in an oscillator circuit, where curvature-induced stress within the material changes the amplitude and the frequency of the output signal of the circuit.

Using finite element modelling and experimental methods to investigate planar coil sensor topologies for inductive measurement of displacement

The usage of planar sensors is widespread due to their non-contact nature and small size profiles, however only a few basic design types are generally considered. In order to develop planar coil designs we have performed extensive finite element modelling (FEM) and experimentation to understand the performance of different planar sensor topologies when used in inductive sensing. We have applied this approach to develop a novel displacement sensor. Models of different topologies with varying pitch values have been analysed using the ANSYS Maxwell FEM package, furthermore the models incorporated a movable soft magnetic amorphous ribbon element. The different models used in the FEM were then constructed and experimentally tested with topologies that included mesh, meander, square coil, and circular coil configurations. The sensors were used to detect the displacement of the amorphous ribbon. A LabView program controlled both the displacement stage and the impedance analyser, the latter capturing the varying ...

Comparison between Modelled and Measured Magnetic Field Scans of Different Planar Coil Topologies for Stress Sensor Applications

The investigation of planar coils of differing topologies, when combined with a magnetostrictive amorphous ribbon to form a stress-sensitive self-inductor, is an active research area for applications as stress or pressure sensors. Four topologies of planar coil (Circular, Mesh, Meander, and Square) have been constructed using copper track on 30 mm wide PCB substrate. The coils are energized to draw 0.4 A and the resulting magnetic field distribution is observed with a newly developed three-dimensional magnetic field scanner. The system is based on a variably angled Micromagnetics® STJ-020 tunneling magneto-resistance sensor with a spatial resolution of 5–10 µm and sensitivity to fields of less than 10 A/m. These experimental results are compared with the fields computed by ANSYS Maxwell® finite element modelling of the same topologies. Measured field shape and strength correspond well with the results of modelling, including direct observation of corner and edge effects. Three-dimensional analysis of the field shape produced by the square coil, isolating the components H(x) and H(z), is compared with the three-dimensional field solutions from modelling. The finite element modelling is validated and the accuracy and utility of the new system for three-dimensional scanning of general stray fields is confirmed.