Paul Z. Goldstein

Integrating DNA barcode data and taxonomic practice: Determination, discovery, and description

DNA barcodes, like traditional sources of taxonomic information, are potentially powerful heuristics in the identification of described species but require mindful analytical interpretation. The role of DNA barcoding in generating hypotheses of new taxa in need of formal taxonomic treatment is discussed, and it is emphasized that the recursive process of character evaluation is both necessary and best served by understanding the empirical mechanics of the discovery process. These undertakings carry enormous ramifications not only for the translation of DNA sequence data into taxonomic information but also for our comprehension of the magnitude of species diversity and its disappearance. This paper examines the potential strengths and pitfalls of integrating DNA sequence data, specifically in the form of DNA barcodes as they are currently generated and analyzed, with taxonomic practice.

Pitfalls in phylogenetic analysis of large molecular data sets

Fundamental considerations of phylogenetic analysis are reviewed in the context of treating large molecular matrices. While molecular data sets with dozens or hundreds of taxa are increasingly common in phylogenetic inference studies, several computational issues, some unique to such large matrices, others general in phylogenetic inference, nonetheless confront molecular systematists. The most controversial of these, choice among phylogenetic inference methods, bears directly on the analysis of molecular data sets. Maximum likelihood methods have been implemented exclusively for molecular data, but their burdensome computational load becomes acute as the number of taxa being analyzed grows. While there are several reasons to prefer parsimony to maximum likelihood generally, the unfeasibility of using likelihood to treat matrices with many terminals and the desirability of combining morphological and molecular under simultaneous analysis lead to a preference for parsimony more or less by default. Terminal selection and the coding of subset polymorphisms and inapplicable character data are of no less critical concern to molecular systematists than to morphologists. Shortcuts such as collapsing taxa to form “composite” terminals should be viewed with caution. Measures of nodal support, all of which are problematic in one or more ways, may be computationally prohibitive for large matrices. The relatively novel technique of parsimony jacknifing may provide a desirable means of evaluating the robustness of phylogenetic inference, especially as the generation of sequence data becomes increasingly routine.

Transcriptome profiling with focus on potential key genes for wing development and evolution in Megaloprepus caerulatus, the damselfly species with the world's largest wings

The evolution, development and coloration of insect wings remains a puzzling subject in evolutionary research. In basal flying insects such as Odonata, genomic research regarding bauplan evolution is still rare. Here we focus on the world’s largest odonate species—the “forest giant” Megaloprepus caerulatus, to explore its potential for looking deeper into the development and evolution of wings. A recently discovered cryptic species complex in this genus previously considered monotypic is characterized by morphological differences in wing shape and color patterns. As a first step toward understanding wing pattern divergence and pathways involved in adaptation and speciation at the genomic level, we present a transcriptome profiling of M. caerulatus using RNA-Seq and compare these data with two other odonate species. The de novo transcriptome assembly consists of 61,560 high quality transcripts and is approximately 93% complete. For almost 75% of the identified transcripts a possible function could be assigned: 48,104 transcripts had a hit to an InterPro protein family or domain, and 28,653 were mapped to a Gene Ontology term. In particular, we focused on genes related to wing development and coloration. The comparison with two other species revealed larva-specific genes and a conserved ‘core’ set of over 8,000 genes forming orthologous clusters with Ischnura elegans and Ladona fulva. This transcriptome may provide a first point of reference for future research in odonates addressing questions surrounding the evolution of wing development, wing coloration and their role in speciation.

De Novo characterization of transcriptomes from two North American Papaipema stem-borers (Lepidoptera: Noctuidae)

Stem-borers in the genus Papaipema (Lepidoptera: Noctuidae) range from highly polyphagous agricultural pests to specialists on more than 20 families of flowering plants, many of them highly toxic. Papaipema is the largest genus of noctuids endemic to North America and provides an excellent study system for the evolution of noctuid host plant use. To improve the availability of genomic resources for such investigations, we performed de novo transcriptome sequencing and assembly for two specialist Papaipema with unusual larval hosts: P. speciosissima, which is associated with ferns, and the undescribed P. “sp. 4,” which is associated with bamboo. The resulting transcriptomes were similar in terms of completeness, gene count, and gene identity, but we identified some 8,000 genes (~17% of each transcriptome) not shared between the two species. While some of these have identifiable orthologs in other Lepidoptera, ~5% of each transcriptome consists of species-specific genes. We examine the function of these genes and find that almost half have retrotransposon-related functional domains. The potential role of species-specific genes is discussed, and the expansion of certain retrotransposon families in Papaipema is examined.