Paula Guedes

Phosphorus recovery from sewage sludge ash through an electrodialytic process

The electrodialytic separation process (ED) was applied to sewage sludge ash (SSA) aiming at phosphorus (P) recovery. As the SSA may have high heavy metals contents, their removal was also assessed. Two SSA were sampled, one immediately after incineration (SA) and the other from an open deposit (SB). Both samples were ED treated as stirred suspensions in sulphuric acid for 3, 7 and 14 days. After 14 days, phosphorus was mainly mobilized towards the anode end (approx. 60% in the SA and 70% in the SB), whereas heavy metals mainly electromigrated towards the cathode end. The anolyte presented a composition of 98% of P, mainly as orthophosphate, and 2% of heavy metals. The highest heavy metal removal was achieved for Cu (ca. 80%) and the lowest for Pb and Fe (between 4% and 6%). The ED showed to be a viable method for phosphorus recovery from SSA, as it promotes the separation of P from the heavy metals.

Electrokinetic remediation of six emerging organic contaminants from soil

Some organic contaminants can accumulate in organisms and cause irreversible damages in biological systems through direct or indirect toxic effects. In this study the feasibility of the electrokinetic (EK) process for the remediation of 17β-oestradiol (E2), 17α-ethinyloestradiol (EE2), bisphenol A (BPA), nonylphenol (NP), octylphenol (OP) and triclosan (TCS) in soils was studied in a stationary laboratory cell. The experiments were conducted using a silty loam soil (S2) at 0, 10 and 20mA and a sandy soil (S3) at 0 and 10 mA. A pH control in the anolyte reservoir (pH>13) at 10 mA was carried out using S2, too. Photo and electrodegradation experiments were also fulfilled. Results showed that EK is a viable method for the remediation of these contaminants, both through mobilization by electroosmotic flow (EOF) and electrodegradation. As EOF is very sensible to soil pH, the control in the anolyte increased EOF rate, consequently enhancing contaminants mobilization towards the cathode end. The extent of the mobilization towards the electrode end was mainly dependent on compounds solubility and octanol-water partition coefficient. In the last 24h of experiments, BPA presented the highest mobilization rate (ca. 4 μg min(-1)) with NP not being detected in the catholyte. At the end of all experiments the percentage of contaminants that remained in the soil ranged between 17 and 50 for S2, and between 27 and 48 for S3, with no statistical differences between treatments. The mass balance performed showed that the amount of contaminant not detected in the cell is similar to the quantity that potentially may suffer photo and electrodegradation.

Valorisation of ferric sewage sludge ashes: potential as a phosphorus source

Sewage sludge ashes (SSA), although a waste, contain elements with socio-economic and environmental potential that can be recovered. This is the case of phosphorus (P). SSA from two Danish incinerators were collected during two years and characterized. The sampling was done immediately after incineration (fresh SSA) or from an outdoor deposit (deposited SSA). Although morphology and mineral composition were similar, physico-chemical and metal concentration differences were found between incinerator plants and sampling periods. No differences were observed between deposited and fresh SSA, except for the parameters directly influenced by disposal conditions (e.g. moisture content). All the SSAs had high concentrations of P (up to 16wt%), but they all exceeded Danish EPA Cd and Ni thresholds for direct application at agricultural soil. Fresh and deposited SSA were acid washed aiming P extraction, achieving 50gP/kg (approx. 37% of total P), but metals were also co-extracted to the liquid phase. To avoid and/or minimize the metals pollution of the extracted P, selective P recovery from the SSA was tested, using the electrodialytic (ED) process. ED laboratory cells, with 3 compartments (3c) and 2 compartments (2c), and two acid concentrations (H2SO4, 0.08M and 0.19M) were used for 7days. The most concentrated acid solution increased P solubilization. The 2c-cell combined with the higher acid concentration resulted in higher P recoveries, 125g of P/kg of SSA in the anolyte. The obtained results showed that the ED process is a valuable tool for the SSA valorisation as it promotes simultaneous P recovery and metals extraction from the SSA.

Electrodialytic treatment of sewage sludge: influence on microbiological community

This study aimed to evaluate the changes in microbiological communities present in sewage sludge (SS) when subjected to an electric field. The establishment of these relations is important to design and optimize an electrotechnology that not only promotes a direct degradation of the emerging organic contaminants, through electrodegradation, but also stimulates their biodegradation. Different current intensities were used continuously or by steps, in a two compartment electrodialytic (ED) cell. Samples collected between May and July of 2015 were analysed, prior to ED treatment, and the 35 Taxa identified were divided in 12 groups. Initial samples, collected on May, were mainly constituted by stalked ciliates (86%), followed by shelled amoebae (8%), whereas the samples collected between June and July presented higher number of shelled amoebae, between 52 and 92%. Epistylis, Vorticella and Arcella gibbosa were the most frequent protozoa identified. Samples richness (R) was between 7 and 14, Simpson’s biodiversity index (D) between 0.45 and 0.69, and evenness between 0.42 and 0.65. At the end of the ED experiments, a decrease in the number of identified Taxa and individuals was observed and, consequently, SS biodiversity also decreased. This decline was more pronounced in the ED experiments conducted with currents above 50xa0mA (0.10xa0mAxa0cm−2). In terms of abundance, the final SS was mainly constituted by shelled amoebae that showed to be the most resistant to the conditions within the ED cell. The obtained results showed that the studied conditions within the ED cell were not appropriate to the microbiological communities, which influenced the final SS quality.

Comparative assessment of LECA and Spartina maritima to remove emerging organic contaminants from wastewater

The present work aimed to evaluate the capacity of constructed wetlands (CWs) to remove three emerging organic contaminants with different physicochemical properties: caffeine (CAF), oxybenzone (MBPh), and triclosan (TCS). The simulated CWs were set up with a matrix of light expanded clay aggregates (LECA) and planted with Spartina maritima, a salt marsh plant. Controlled experiments were carried out in microcosms using deionized water and wastewater collected at a wastewater treatment plant (WWTP), with different contaminant mass ranges, for 3, 7, and 14xa0days. The effects of variables were tested isolatedly and together (LECA and/or S. maritima). The presence of LECA and/or S. maritima has shown higher removal (around 61–97%) of lipophilic compounds (MBPh and TCS) than the hydrophilic compound (CAF; around 19–85%). This was attributed to the fact that hydrophilic compounds are dissolved in the water column, whereas the lipophilic ones suffer sorption processes promoting their removal by plant roots and/or LECA. In the control (only wastewater), a decrease in the three contaminant levels was observed. Adsorption and bio/rhizoremediation are the strongest hypothesis to explain the decrease in contaminants in the tested conditions.

Removal of Pharmaceutical and Personal Care Products in Aquatic Plant-Based Systems

The use of pharmaceuticals and personal care products (PPCPs) is growing as they are used not only for treatment but also for prevention of illnesses. In wastewater treatment plants (WWTPs) many PPCPs are not completely degraded/removed, which contributes for their frequent detection in, e.g. salt marsh areas. These areas may be considered a sink, source and cycling centre of contaminants on the receiving water body flow rate. In this chapter, a study about the potential of salt marsh plants Halimione portulacoides and/or Spartina maritima for the removal of three PPCPs, caffeine (CAFF), oxybenzone (HMB) and triclosan (TCS), is summarized. Experiments, at microcosms level, had two main aims: (1) the simulation of natural estuarine environment and study of dispersion mechanisms and “natural” remediation potential of target PPCPs and (2) the optimization of biological treatment technologies in WWTPs by simulating constructed wetland composed by plants and light expanded clay aggregates (LECA). Results indicate that either in estuaries or in WWTP the presence of a physical support can help to decrease contaminant levels mainly through sorption processes. The tested salt marsh species also showed potential to directly or indirectly promote the remediation of contaminants. In any case the remediation potential and dispersion mechanism are highly dependent from the characteristics of contaminants, like octanol–water partition coefficient (Log Kow) and solubility.

Incorporation of Different Fly Ashes from MSWI as Substitute for Cement in Mortar: An Overview of the Suitability of Electrodialytic Pre-treatment

Municipal solid waste incineration (MSWI) is a widely used technique for waste management, as it significantly reduces the volume of waste and energy is gained. However, MSWI may cause environmental problems, mostly related to the disposal of the produced hazardous fly ash (FA). There are different types of FA that result from cleaning emissions or burned municipal solid waste, and their hazardousness is due to their high contaminant content, namely leachable heavy metals, salts, and/or organic contaminants. Nevertheless, FA from MSWI can be used, e.g., in the production of construction materials, if the contaminants are successfully removed or stabilized. The goal of this chapter is to make an overview and understand if the electrodialytic (ED) process could be used as a pre-treatment method for FA, before the incorporation in Ordinary Portland Cement. The review showed that ED process may be a viable method for FA waste reuse, presenting great advantages in the waste management system.

Choroid plexus is an additional source of melatonin in the brain

The cerebrospinal fluid melatonin is released from the pineal gland, directly into the third ventricle, or produced de novo in the brain from extrapineal melatonin sources leading to a melatonin concentration gradient in the cerebrospinal fluid. Despite the interest on this topic, the brain areas capable of producing melatonin are not yet clear. Bearing this in mind, we hypothesized that the choroid plexus (CP) could be one of these melatonin sources. We analyzed and confirmed the presence of the four enzymes required for melatonin synthesis in rat CP and demonstrated that arylalkylamine N‐acetyltransferase shows a circadian expression in female and male rat CP. Specifically, this enzyme colocalizes with mitochondria in rat CP epithelial cells, an organelle known to be involved in melatonin function and synthesis. Then, we demonstrated that melatonin is synthesized by porcine CP explants, although without a circadian pattern. In conclusion, our data show that the CP is a local source of melatonin to the central nervous system, probably contributing to its high levels in the cerebrospinal fluid. We believe that in the CP, melatonin might be regulated by its endogenous clock machinery and by the hormonal background.

Electrodialytic 2-compartment cells for emerging organic contaminants removal from effluent

The present work discusses the efficiency of the electrodialytic (ED) process to remove emerging organic contaminants (EOCs) from effluent. The ED process was carried out in a cell of two-compartments (2u202fC-cell) with effluent in either the anode or cathode compartment, separated from the electrolyte compartment through an anion or a cation exchange membrane (AEM and CEM, respectively). As effluent destination might be soil irrigation, and having in mind the nutrient recycling, phosphorus was also monitored in the process. The ED removals showed to be dependent of EOCs characteristics and cell design. Removals were higher when using an AEM (60-72%) than a CEM (8-63%), except for caffeine when the effluent was placed in the cathode, that did not show any removal. When using an AEM with the effluent placed in the anode compartment, all the EOCs (including caffeine) were removed between 57-72%, mainly through electrodegradation phenomena. Regarding phosphorus, a polarity switch may be done to a 2u202fC-cell with a AEM, depending on the effluent final use. This technology is still in its first steps and, in both cases, further optimization of ED parameters is needed. Still, this technological innovation and cross-cutting research envisages the promotion of economic, social and environmental benefits.

Remediation potential of caffeine, oxybenzone, and triclosan by the salt marsh plants Spartina maritima and Halimione portulacoides

Pharmaceuticals and personal care products (PPCPs) have attracted increasing concern during the last decade because of their widespread uses and continuous release to the aquatic environment. This work aimed to study the distribution of caffeine (CAF), oxybenzone (MBPh), and triclosan (TCS) when they arrive in salt marsh areas and to assess their remediation potential by two different species of salt marsh plants: Spartina maritima and Halimione portulacoides. Experiments were carried out in the laboratory either in hydroponics (sediment elutriate) or in sediment soaked in elutriate, for 10xa0days. Controls without plants were also carried out. CAF, MBPh, and TCS were added to the media. In unvegetated sediment soaked in elutriate, CAF was mainly in the liquid phase (83%), whereas MBPh and TCS were in the solid phase (90% and 56%, respectively); the highest remediation was achieved for TCS (40%) and mainly attributed to bioremediation. The presence of plants in sediment soaked in elutriate-enhanced PPCPs remediation, decreasing CAF and TCS levels between approximately 20-30% and MBPh by 40%.. Plant uptake, adsorption to plant roots/sediments, and bio/rhizoremediation are strong hypothesis to explain the decrease of contaminants either in water or sediment fractions, according to PPCPs characteristics.