Pavel A. Fomitchov

Multispectral line confocal imaging microscope for fluorescence applications

We present a novel line confocal microscope for investigating biological samples that simultaneously acquires images from three fluorescent channels. This instrument provides the majority of the benefits of confocal microscopes, such as depth sectioning and improved contrast, while allowing more of the excitation illumination to reach the sample area, resulting in reduced exposure times. One configuration of the line confocal fluorescent microscope is designed to work with a four laser illuminator, and three discrete cameras, while an alternate configuration allows the instrument to act as a multispectral microscope that captures the fluorescent emission over the visible and NIR wavelength range.

Dual-modality imager based on ultrasonic modulation of incoherent light in turbid medium

A hybrid imaging system capable of combining the spatial resolution of ultrasound with functional sensitivity of fluorescence optical imaging can render the conventionally ill-posed optical image reconstruction more tractable. In this paper, ultrasonic modulation of diffuse photons from an incoherent source in a turbid medium or FluoroSound has been proposed as a mechanism for dual-modality fusion. Theoretical calculations based on a diffusion approximation for tissue depths up to 2cm and reduced scattering coefficients ranging from 5-20/cm have shown show that diffuse photon modulation increases with scattering, decreases with absorption, reaches a minimum when the acoustic focus is located mid-way between the source and the detector; and can be optimized by a suitably shaped and sized acoustic focus. The diffuse photon modulation signature could potentially be used for improving spatial resolution of deep tissue fluorescence imaging and enable fusion of ultrasound and optical imaging in a single measurement