Pcs Tsang
University of Hong Kong
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Publication
Featured researches published by Pcs Tsang.
Apmis | 2006
Nb Parahitiyawa; Yh Samaranayake; Lp Samaranayake; J. Ye; Pcs Tsang; Bpk Cheung; J. Y. Y. Yau; Skw Yeung
An in vitro assay to study multiple Candida biofilms, in parallel, has been carried out using the Calgary biofilm device (CBD). We here report: i) standardization of the CBD for Candida albicans biofilm formation, ii) kinetics of C. albicans biofilm formation, iii) biofilm formation by five Candida species, and iv) effect of dietary carbohydrates on biofilm formation. The biofilm metabolic activity on all CBD pegs was similar (p=0.6693) and C. albicans biofilm formation revealed slow growth up to 36 h and significantly higher growth up to 48 h (p<0.001). Significant differences in total biofilm metabolic activity were seen for glucose, fructose and lactose grown C. albicans compared with sucrose and maltose grown yeasts. Candida krusei developed the largest biofilm mass (p<0.05) relative to C. albicans, C. glabrata, C. dubliniensis and C. tropicalis. Scanning electron microscopy revealed that C. krusei produced a thick multilayered biofilm of pseudohyphal forms embedded within the polymer matrix, whereas C. albicans, C. dubliniensis and C. tropicalis biofilms consisted of clusters or chains of cells with sparse extracellular matrix material. We conclude that CBD is a useful, simple, low cost miniature device for parallel study of Candida biofilms and factors modulating this phenomenon.
Medical Mycology | 1997
By Cao; Pcs Tsang; T Wu; Lp Samaranayake; J Wang
Candida krusei CK 18 carries heterozygosity for a recessive adenine biosynthesis allele, as it can be induced by UV irradiation to form adenine mutants at high frequency. The resultant mutants were characterized with the phenotype of red colony and residual growth on minimal media. The majority of the descendant prototrophic segregates were capable of continuous segregation to adenine mutants, suggesting gene conversion as one possible mechanism for the occurrence of the adenine auxotroph in the isolate.
Archive | 2015
Sm Bridges; Jl Green; Mg Botelho; Pcs Tsang
Microbios | 1996
By Cao; Pcs Tsang; Dnc Tsang; Lp Samaranayake; J Wang
Archive | 1995
Pcs Tsang; Lp Samaranayake; Ss Lee; P Li; Raj G. Nair
Archive | 2015
H Yang; Pcs Tsang; Olt Lam; Ehn Pow; Wk Tsang
Archive | 2014
H Yang; Pcs Tsang; Ehn Pow; Olt Lam; Wk Tsang
Archive | 2014
H Yang; Pcs Tsang; Ehn Pow; Pwk Tsang
Archive | 2013
Sm Bridges; Cky Yiu; Mg Botelho; Ecm Lo; Pcs Tsang; Rm Watt; Y Yang; Jw Wong
Archive | 2013
Ehn Pow; Ecm Tse; Pcs Tsang