Pearl R. Anderson

Effects of Intravenous Infusions of Feather Keratin Preliminary Characterization and Evaluation as a Plasma Expander.

Summary A peroxide hydrolysate of feather keratin has received preliminary evaluation as a plasma volume expander. Intravenous infusions in dogs produced acute toxic effects characterized by hemoconcentration, leucopenia, thrombocytopenia, methemoglobinemia, oliguria and lethargy. The results indicate that peroxide hydrolysates obtained from chicken feathers are unsuitable for intravenous use.

Carbohydrate studies in tropical sprue

The lactose tolerance test is useful for detecting malabsorption in tropical sprue. For this purpose it is superior to a number of other oral tolerance tests. The generalized disaccharidase deficiency found in tropical sprue appears to be a secondary phenomenon resulting from injury to the intestinal mucosa.SummaryThe lactose tolerance test is useful for detecting malabsorption in tropical sprue. For this purpose it is superior to a number of other oral tolerance tests. The generalized disaccharidase deficiency found in tropical sprue appears to be a secondary phenomenon resulting from injury to the intestinal mucosa.

Effect of tourniquet injury on the tyrosine oxidizing system of liver of rats

Abstract Tourniquet injury in rats produces an inhibition of the tyrosine oxidizing system in rat liver. There is an accumulation of p -hydroxyphenylpyruvate and an increased requirement for ascorbic acid.

Purification of pancreatic and urinary callicrein.

Summary A method has been devised for preparation of highly purified pancreatic callicrein. The fractionation procedure involves three ethanolic fractionations at pH 5.5, 7.5. and 6.5 followed by one ammonium sulfate precipitation. Solutions of hypotensive enzyme at this level of purity proved highly unstable and further purification was abandoned. Preliminary purification of urinary callicrein revealed that the method could be adapted to this source.

Enzyme-inhibitor complex of the tyrosine-α-ketoglutarate transaminase in rat liver

Abstract Tyrosine α-ketoglutaric transaminase in extracts from liver tissue from albino rats subjected to tourniquet injury was three times more active than that from normal animals. Its properties also differed from those of the enzyme in normal extracts indicating an actual change in the enzyme system rather than an increase of normal enzyme. Electrophoretic studies showed that the normal system contained two components with enzymic activity, one of these being increased to a much greater extent than the other in the traumatic state. E V vs. E plot of data is consistent with the interpretation that an inhibitor was present in the normal state and was decreased on injury to the animal. Kinetic studies are consistent with the interpretation that rate of reaction of tyrosine with pyridoxal phosphate, one of the two steps of the transaminase reaction, was increased in traumatized animals.