Pedro Miguel Ocampos Pedroso

Rabies in the Insectivorous Pallas's Mastiff Bat (Molossus molossus) in Northeastern Brazil

Abstract We describe the pathologic and immunohistochemical findings in five cases of rabies in the insectivorous Pallass mastiff bat (Molossus molossus) in the city of Patos, state of Paraíba, northeastern Brazil. All cases were found during the day, in different neighborhoods, prostrate and unable to fly. No significant lesions were observed at necropsy or on histologic examination. The brains of the five bats showed strong immunohistochemical labeling for rabies virus in the form of large corpuscles or multiple clusters of granules within the perikarya of neurons in different areas of the brain, mainly in the cerebral cortex and cerebellar Purkinje cells. Three bats tested by direct immunofluorescence and mouse inoculation were also positive for rabies. These data demonstrate that M. molossus may be infected with the rabies virus and develop rabies, even without histologic lesions in the central nervous system, and can be a source of infection for humans and domestic animals.

Intoxicação por Metternichia princeps (Solanaceae) em caprinos no Estado da Bahia

ABSTRACT.- Pedroso P.M.O., Santos M.V.B., Seus V.G., Silva R.M.M., Riet-Correa F. & Macedo J.T.S.A. 2015. [ Poisoning by Metternichia princeps (Solanaceae) in goats in the State of Bahia, Brazil. ] Intoxicacao por Metternichia princeps (Solanaceae) em caprinos no Esta-do da Bahia. Pesquisa Veterinaria Brasileira 35(5):448-450 . Setor de Patologia Veterinaria, Universidade Federal do Reconcavo da Bahia, Campus Universitario, Rua Rui Barbosa 710, Cruz das Almas, BA 44380-000, Brazil. E-mail: pedrosovet@yahoo.com.brAn outbreak of poisoning by Metternichia princeps is reported in goats from the State of Bahia. Out of eight goats three showed symptoms of poisoning and died; on two of them post-mortem examinations were performed. The main clinical signs were nasal mucous secretion, weight loss, diarrhea, lethargy, mild weakness, staggering gait, flexion of the fore and hind limbs, sternal recumbence, and lateral recumbence followed by death after approximately two days of clinical manifestations. The serum concentration of urea and creatinine and the serum activities of creatine phosphokinase were increased. At necropsy pulmonary edema, hydrothorax, hydropericardium, ascites, pale kidneys, perirenal edema and hemorrhages in the subcutaneous tissue were observed. Microscopically the kidneys showed accentuated coagulation necrosis of the tubular epithelium and tubules and epi-thelial regeneration. In the lungs there was accentuated congestion associated with inter-alveolar and interseptal edema.

Forma nervosa da artrite-encefalite caprina

Two cases are described of female goats, Saanen crossbreds, two months old, with clinical signs of hind limb paresis, incoordination, difficulty breathing and cachexia, which were subjected to euthanasia due to unfavorable prognosis. At necropsy it was possible to observe that there was congestion of the central nervous system (CNS) and pulmonary consolidation in both goats. Microscopic changes were characterized by multifocal lymphoplasmacytic leucoencephalomielitis, with vacuolization of neuropile and axonal spheroids, leptomeningitis, interstitial nonsupurative pneumonia in goat A and bacterial bronchopneumonia in goat B. Immunohistochemistry (IHC) was positive for the caprine arthritis - encephalitis virus in thalamus fragments. The agar gel immunodiffusion (AGID) test was performed in sera sample of twenty animals and sixteen resulted positive. Diagnosis of the nervous form of caprine arthritis-encephalitis virus (CAEV) infection was based on clinical and pathological status, IHC and AGID. This clinical presentation of CAE could be uncommon in Brazil, but must be considered in case of neurologic diseases in small ruminants.