Penny Hawken

Do cyclic female goats respond to males with an increase in LH secretion during the breeding season

The male effect is currently only used during seasonal or lactational anoestrus because the response is thought to be blocked in cyclic females by periods of elevated progesterone. In this study, we tested whether cyclic, female goats would respond to male exposure with an increase in pulsatile LH secretion. During May (breeding season; Southern Hemisphere) the cycles of 16 Australian Cashmere goats were synchronised using intravaginal progesterone pessaries. Pessary insertion was staggered to produce groups in their early luteal (EL; n=8) and late luteal phases (LL; n=8). The LL group was retrospectively subdivided into mid-luteal (ML; n=4) and late luteal (LL; n=4) groups due to differences in oestrous cycle length that emerged during the study. Male exposure stimulated an increase in LH pulse frequency in the EL and LL groups (P<0.01) but not in the ML group (P>0.1). This increase was accompanied by an increase in basal and mean concentrations of LH in the LL group (P<0.05) but not in EL (P<0.1) or ML (P>0.1) group. There was no effect of male exposure on LH pulse amplitude (P>0.1). Progesterone concentrations differed among all groups on the day of male exposure (P<0.05) and declined significantly over the 12-h sampling period in the LL group (P<0.05). Prolactin concentrations declined in the EL group but did not change significantly in the ML or LL group. In conclusion, male exposure induced an increase in pulsatile LH in goats in the early and late luteal phases of the oestrous cycle. The high concentrations of progesterone in females in the mid-luteal phase appeared to block the male effect.

Genetic selection for temperament affects behaviour and the secretion of adrenal and reproductive hormones in sheep subjected to stress

We investigated the effect of genetic selection for temperament on the way that stressors affect the behaviour and the adrenal and reproductive axes of sheep. We tested three hypotheses: (i) isolation would increase cortisol secretion and decrease luteinising hormone (LH) secretion more in nervous sheep than in calm sheep; (ii) isolation combined with simulated human presence would increase cortisol secretion and decrease LH secretion more in nervous sheep than in calm sheep and (iii) isolation combined with stressors that were not specific to the selection process (i.e. non-selection stressors) would increase cortisol secretion and decrease LH secretion equally in calm and nervous sheep. Isolation alone increased cortisol secretion and decreased LH secretion in nervous sheep but not in calm sheep. Compared to calm sheep, nervous sheep were more agitated during the first 2 h of isolation but not during the second 2 h of isolation. Exposure to non-selection stressors increased cortisol secretion, decreased LH pulse amplitude and the mean plasma concentrations of LH in both calm and nervous sheep. We conclude that genetic selection for temperament affects the behavioural expression of the stress response and the secretion of adrenal and reproductive hormones during isolation, but has less impact on their reactivity to non-selection stressors.

Nutrition affects Sertoli cell function but not Sertoli cell numbers in sexually mature male sheep

We tested whether the reversible effects of nutrition on spermatogenesis in sexually mature sheep were mediated by Sertoli cells. Rams were fed with diets designed to achieve a 10% increase (High), no change (Maintenance) or a 10% decrease (Low) in body mass after 65 days. At the end of treatment, testes were lighter in the Low than the High group (PP<0.05) in the expression of seven Sertoli cell-specific genes. Under-nutrition appeared to reverse cellular differentiation leading to disruption of tight-junction morphology. In conclusion, in sexually mature sheep, reversible reductions in testis mass and spermatogenesis caused by under-nutrition were associated with impairment of basic aspects of Sertoli cell function but not with changes in the number of Sertoli cells.

Socio-Sexual Stimuli and Reproductive Function: Emerging Perspectives of the Male Effect in Sheep and Goats

Producers are facing increasing public scrutiny of the methods used in the production of animal products. Concerns over hormone residues in meat and milk may lead to restricted use of exogenous hormones in the reproductive management of farm animals in the future, so it is important to develop non-pharmacological methods of oestrus synchronisation. The ‘male effect’ was first reported in sheep in the 1940s and describes the capacity of the male to increase the secretion of reproductive hormones and induce ovulation in the female. It is widely accepted that olfactory signals (ostensibly ‘pheromones’) are primarily responsible for the profound shift in the activity of the reproductive centres of the female brain, though the chemical nature of this signal is yet to be conclusively identified for either sheep or goats. On the other hand, our understanding of the mechanism through which socio-sexual stimuli stimulate the hypothalamic-pituitary-gonadal axis of females has been greatly improved through a combination of histological, electrophysiological and endocrinological techniques. It is clear that olfactory stimuli from males are primarily transmitted through the main olfactory system, with audio and visual stimuli playing a synergistic but relatively minor role. In spite of over 60 years of research in this field, there are several areas of current and potential research that should improve our understanding of this remarkable phenomenon and its application to farm animal management.