Pere Casals

CO2 efflux from a Mediterranean semi-arid forest soil. I. Seasonality and effects of stoniness.

We studied the seasonality of total soil CO2efflux and labeled C-CO2 released from 14Clabeled straw incubated in the H horizon of asemi-arid Mediterranean forest soil. Fieldmeasurements were carried out over 520 days in aseries of reconstructed soil profiles with and withouta gravel layer below the H horizon. We monitored soilclimate and related this to soil CO2 efflux.Seasonal variations in soil CO2 efflux in asemiarid Mediterranean forest were mainly related tochanges in soil temperature. In spite of drought, highrespiration rates were observed in mid summer. Highsoil CO2 efflux in hot and dry episodes wasattributed to increases in soil biological activity.The minimum soil CO2 efflux occurred in latesummer also under dry conditions, probably related toa decrease in soil biological activity in deephorizons. Biological activity in organic layers waslimited by water potential (Ψ) in summer and bytemperature in winter. Rewetting a dry soil resultedin large increases in soil CO2 efflux only at hightemperatures. These large increases represented asignificant contribution to the decomposition oforganic matter in the uppermost horizons. Soilbiological activity in the uppermost horizons was moresensitive to changes in soil Ψ and hence tosummer rainstorms than the bulk soil microbialactivity. The presence of a layer of gravel improvedboth moisture and temperature conditions for thedecomposition of organic matter. As a result, soilCO2 efflux increased in soils containing rockfragments. These effects were especially large for theorganic layers.

Decomposition of 13C-labelled standard plant material in a latitudinal transect of European coniferous forests: Differential impact of climate on the decomposition of soil organic matter compartments

Abstract13C labelled plant material was incubated in situ over 2 to 3 years in 8 conifer forest soils located on acid and limestone parent material along a north-south climatic transect from boreal to dry Mediterranean regions in western Europe. The objectives of the experiment were to evaluate the effects of climate and the soil environment on decomposition and soil organic matter dynamics. Changes in climate were simulated using a north-to-south cascade procedure involving the relocation of labelled soil columns to the next warmer site along the transect.Double exponential, decay-rate functions (for labile and recalcitrant SOM compartments) vs time showed that the thermosensitivity of microbial processes depended on the latitude from which the soil was translocated. Cumulative response functions for air temperature, and for combined temperature and moisture were used as independent variables in first order kinetic models fitted to the decomposition data. In the situations where climatic response functions explained most of the variations in decomposition rates when the soils were translocated, the climate optimised decomposition rates for the local and the translocated soil should be similar. Differences between these two rates indicated that there was either no single climatic response function for one or both compartments, and/or other edaphic factors influenced the translocation effect. The most northern boreal soil showed a high thermosensitivity for recalcitrant organic matter compartment, whereas the labile fraction was less sensitive to climate changes for soils from more southern locations. Hence there was no single climatic function which describe the decay rates for all compartments. At the end of the incubation period it was found that the heat sum to achieve the same carbon losses was lower for soils in the north of the transect than in the south. In the long term, therefore, for a given heat input, decomposition rates would show larger increases in boreal northern sites than in warm temperate regions.The changes in climate produced by soil translocation were more clearly reflected by decomposition rates in the acid soils than for calcareous soils. This indicates that the physicochemical environment can have important differential effects on microbial decomposition of the labile and recalcitrant components of SOM.

Aboveground litter quality changes may drive soil organic carbon increase after shrub encroachment into mountain grasslands

Shrub encroachment into grasslands is ubiquitous but its impact on soil organic C (SOC) remains unclear. In previous work we had observed that shrub encroachment into mesic mountain grasslands increased SOC content. Here we sought the mechanisms of this increase. To this end, we assessed aboveground and belowground production for a conifer shrub (Juniperus communis L), a legume shrub (Cytisus balansae ssp. europaeus (G. López & Jarvis) Muñoz Garmendia) and grass (Festuca eskia Ramond ex DC), together with decomposition rates for both aboveground litter and roots. Belowground C net inputs do not clearly explain SOC increase: grass root production was higher than that of either shrub and the decomposition rate of grass roots was the lowest. Aboveground C net inputs were only slightly greater in shrubs than in grass, but the decomposition rate of litter of both shrubs was much lower than that of grass. The decomposition of conifer litter was N-limited, whereas that of legume shrub litter was P-limited. Thus we conclude that the SOC increases after shrub encroachment into mesic grasslands probably as a result of higher recalcitrance of shrub aboveground litter relative to grass litter.

Nitrogen supply rate in Scots pine (Pinus sylvestris L.) forests of contrasting slope aspect

We studied Nitrogen (N) transformations in Pinus sylvestris forest stands in the foothills of the SE Pre-Pyrenees (NE Spain). Plots were selected in two contrasting aspects (two plots per aspect) and N supply rate was measured by the resin-core incubation technique once every three months. N leaching through litter layers (L and F horizons) was evaluated by 5 zero-tension lysimeters in each plot. NH4+-N, NO3--N and soluble organic-N were determined in all solutions. N supply rate showed a clear seasonal pattern. Ammonification and nitrification were segregated in space and in time. While ammonification showed a peak in spring, nitrification was higher in summer. There was evidence suggesting that nitrification occurs mostly in A1 horizon. Nitrification rates differed significantly among plots. N supply rate was 12.7–23.5 kg N·ha-1·yr-1 but it did not differ between aspects or plots. Inorganic-N leached through litter layers was 14–17 kg N·ha-1·yr-1, and represented a high proportion of N supply rate. Organic-N leached through litter layers (27.8–37.0 kg N·ha-1·yr-1) was higher than leached inorganic-N. However, in most cases organic-N did not represent a high proportion of changes in soluble organic-N pools in H and A1 horizons (about 240 kg N·ha-1·yr-1). This large decrease in soluble organic-N was much greater than the increase in inorganic-N. The possible fate of these large amounts of organic-N is discussed.

The stable isotope ecology of terrestrial plant succession

We review the relevance and use of stable isotopes for the study of plant community succession. Stable isotope measurements provide information on the origin of resources acquired by plants, the processes governing resource uptake and transformation, and the physiological and environmental conditions of plant growth. When combined with measurements of the stable isotope ratio values of soil microbial biomass, soil organic matter and plant litter, isotope measurements of plants can indicate effects of successional changes on ecosystem processes. However, their application to questions of plant succession and ecosystem change is limited by the degree to which the underlying assumptions are met in each study, and complementary measures may be required, depending upon the question of interest. First, we discuss the changes that occur in the stable isotope composition of plants and ecosystems with ontogeny and species replacements, as well as their potential evolutionary significance. Second, we discuss the imprints of plant competition and facilitation on leaf and wood tissue, as well as how stable isotopes can provide novel insights on the mechanisms underlying plant interactions. Finally, we discuss the capacity for stable isotope measurements to serve as a proxy record for past disturbances such as fire, logging and cyclones.

Recovery of fresh debris of different sizes in density fractions of two contrasting soils

Fresh plant residues are often identified with the light organic matter obtained by fractionation in a dense liquid, but the extent to which residues can be extracted efficiently by densimetric methods has not been widely studied. This paper presents the results of two experiments in which 14C-labelled straw was mixed with two soils of differing texture, and the mixture was subjected to densimetric fractionation. In the first experiment, soil was mixed with bulk milled straw, and in the second, with straw of different sizes (> 200 μm, 200-50 μm, 50-20 μm and < 20 μm). The recovery of straw in the light fraction was low, and decreased with decreasing straw size, reaching a minimum (less than 20 %) for straw < 20 μm. Except for straw < 20 μm, the recovery in the light fraction was lower in soil richest in silt and clay. In the absence of soil (blanks), the recovery of fine straw in the light fraction was lower than in the presence of soil, suggesting that the recovery of fine straw in the light fraction is partly due to its association with light coarse debris. Extractable and polytungstate-soluble fractions accounted for a small proportion of the 14C-activity. These results suggest that densimetric methods are not efficient for recovering fresh plant residues, except in the case of large residues in coarse-textured soils.

Silvopastoral Systems in the Northeastern Iberian Peninsula: A Multifunctional Perspective

This paper aims to analyse the present situation of silvopastoral systems in the Northeastern Iberian Peninsula and to foresee the role that silvopastoralism might play in the future. In the region, silvopasture form part of an extensive livestock farming system which integrates agriculture, livestock production and forestry, mostly in family-based farms. Forest grazing is the most important silvopastoral system in the study area where the mountainous topography and the dominant Mediterranean climate influence the grazing strategies. Despite the low contribution of silvopastoralism to the total Gross Domestic Product of the Catalan region, extensive livestock systems play an important social and economic role in the structure of rural areas. Moreover, this role is expected to increase in the future due to the implementation of new agrienvironment measures in the framework of the latest European financial program (2007–2013). The present paper discusses the role of silvopastoralism as an economically viable tool to prevent wildfires and conserve biodiversity in these systems.

Spatial patterns of shrub cover after different fire disturbances in the Pyrenees.

Abstract• Woody encroachment into grasslands is a worldwide phenomenon. In the Pyrenees, fire has been used as a management tool to transform part of the encroached land to grassland.• This study aims to compare the spatial patterns of shrub cover 4 y after 4 different fire disturbances (prescribed burning, repeated prescribed burning, wildfire in 20 year-old shrubs and wildfire in 5 year-old shrubs); and also to compare shrub cover after different fire disturbances, accounting for spatial autocorrelation. The study focuses on the shrub Cytisus balansae. Two-dimensional transects (20 × 0.5 m) were established to monitor shrub cover for 4 y after each disturbance type. Autoregressive models and Markov models were used with a Monte Carlo procedure to account for the presence of spatial autocorrelation.• Shrub cover was greater after prescribed burning than after repeated prescribed burning, and it increased with shrub age before disturbance. Differences in spatial patterns were detected in shrub patch size, with repeated prescribed fires and wildfires reducing shrub patch size by half in comparison with prescribed burning.• From the management point of view, the effects of repeated prescribed burning were similar to those of a wildfire on reducing shrub cover and shrub patch size.Résumé• Les empiètements boisés sur les prairies sont un phénomène mondial. Dans les Pyrénées, le feu a été utilisé comme un outil de gestion pour transformer une partie des empiètements boisés en prairies.• Cette étude vise à comparer les caractéristiques spatiales des couverts de buissons 4 ans après 4 différentes perturbations (feu prescrit, feu prescrit répété, feu spontané dans des buissons âgés de 20 ans et feu spontané dans buissons âgés de 5 ans), et aussi de comparer la couverture des buissons après les différents perturbations occasionnées par les incendies, prenant en compte l’autocorrélation spatiale. L’étude se concentre sur les buissons de Cytisus balansae. Deux transects dimensionnels (20 × 0,5 m) ont été mis en place pour suivre la couverture des buissons 4 ans après chaque type de perturbation. Des modèles autorégressifs et des modèles de Markov ont été utilisés avec une procédure de Monte Carlo pour tenir compte de la présence d’une autocorrélation spatiale.• Le couvert des buissons est plus important après le feu prescrit qu’après le feu prescrit répété et il augmente avec l’âge des buissons avant la perturbation. Les différences dans les modèles spatiaux ont été détectées dans la taille des bouquets de buissons, avec les feux prescrits répétés et les feux spontanés réduisant la taille des bouquets de buissons de moitié en comparaison avec le brûlage prescrit.• Du point de vue de la gestion, les effets des feux prescrits répétés ont été similaires à ceux des feux spontanés sur la réduction de la couverture des buissons et de la taille des bouquets de buissons.

CO2 efflux from a Mediterranean semi-arid forest soil. II. Effects of soil fauna and surface stoniness

Many forest soils in the Mediterranean basin areshallow and contain high amounts of gravel in theorganic layers. Recent studies on soil organic matteraccumulation have shown high amounts of organic matteroccurring mainly in soils with high levels ofstoniness at the soil surface. The gravel layer mayaffect the microclimatic conditions of the soilsurface and probably the distribution and activity ofsoil fauna.In order to quantify the combined effects soil fauna(epigeic macrofauna and earthworms) and stoniness onthe release of soil CO2, we performed a threefactor field experiment by using a series ofreconstructed soil profiles. Factors 1 and 2 consistedof the exclusion/presence of soil epigeic macrofaunaand earthworms, and factor 3 of the presence/absenceof a gravel layer intermingled with the H horizon. Weincubated 14C straw in the H horizon and carriedout three 40 mm rainfall simulations.Soil respiration primarily depended on the season. Theeffects of soil fauna were generally small and did notcoincide with periods of high faunal activity. Thelargest effects of both earthworms and soil epigeicfauna were found after wetting the soil in summer. Theeffects of the earthworms were concentrated in themineral soil while the effects of the epigeic faunawere concentrated in the H horizon and mainly arosetowards the end of the experiment. This suggests thatthe effects of epigeic fauna may have beenunderestimated due to the length of the experiment.The gravel layer increased the effect of faunaprobably by creating more favorable microclimaticconditions. The accumulation of organic matter insoils with high levels of stoniness cannot beexplained by the effect of gravel on soil microclimatenor by its effect on the activity of soil fauna.

How Spatial Heterogeneity of Cover Affects Patterns of Shrub Encroachment into Mesic Grasslands

We used a multi-method approach to analyze the spatial patterns of shrubs and cover types (plant species, litter or bare soil) in grassland-shrubland ecotones. This approach allows us to assess how fine-scale spatial heterogeneity of cover types affects the patterns of Cytisus balansae shrub encroachment into mesic mountain grasslands (Catalan Pyrenees, Spain). Spatial patterns and the spatial associations between juvenile shrubs and different cover types were assessed in mesic grasslands dominated by species with different palatabilities (palatable grass Festuca nigrescens and unpalatable grass Festuca eskia). A new index, called RISES (“Relative Index of Shrub Encroachment Susceptibility”), was proposed to calculate the chances of shrub encroachment into a given grassland, combining the magnitude of the spatial associations and the surface area for each cover type. Overall, juveniles showed positive associations with palatable F. nigrescens and negative associations with unpalatable F. eskia, although these associations shifted with shrub development stage. In F. eskia grasslands, bare soil showed a low scale of pattern and positive associations with juveniles. Although the highest RISES values were found in F. nigrescens plots, the number of juvenile Cytisus was similar in both types of grasslands. However, F. nigrescens grasslands showed the greatest number of juveniles in early development stage (i.e. height<10 cm) whereas F. eskia grasslands showed the greatest number of juveniles in late development stages (i.e. height>30 cm). We concluded that in F. eskia grasslands, where establishment may be constrained by the dominant cover type, the low scale of pattern on bare soil may result in higher chances of shrub establishment and survival. In contrast, although grasslands dominated by the palatable F. nigrescens may be more susceptible to shrub establishment; current grazing rates may reduce juvenile survival.