Petek Piner

Oxidative and apoptotic effects of lambda-cyhalothrin modulated by piperonyl butoxide in the liver of Oreochromis niloticus.

The aim of this study was to investigate the toxic effects of pyrethroid pesticide lambda-cyhalothrin in the presence of piperonyl butoxide as a modulator in the liver of juvenile Oreochromis niloticus. LC(50) (96h) value of lambda-cyhalothrin was determined as 2.901μg/L for O. niloticus. The fish were exposed to 0.48μg/L (1/6 of the 96-h LC(50)) lambda-cyhalothrin and 10μg/L piperonyl butoxide for 96-h and 15-d. tGSH, GSH, GSSG, Hsp70 and TBARS contents, GPx, GR, GST and caspase-3 enzymes activities were determined. Lambda-cyhalothrin caused increases in tGSH, GSH, TBARS contents, and GST activity. Piperonyl butoxide treatment with lambda-cyhalothrin caused significant increases in tGSH GSH, Hsp70, TBARS contents, and GPx and GST activities while caspase-3 activity was decreased. The results of the present study revealed that lambda-cyhalothrin caused oxidative stress which upregulated GSH and GSH-related enzymes. Piperonyl butoxide increased the oxidative stress potential and apoptotic effects of lambda-cyhalothrin.

Oxidative stress and apoptosis was induced by bio-insecticide spinosad in the liver of Oreochromis niloticus

This study was conducted to investigate acute toxic effects of spinosad on Glutathione-related oxidative stress markers, lipid peroxidation, heat shock proteins, apoptosis in the liver of Oreochromis niloticus selected as a model organism. The fish were exposed to sublethal spinosad concentrations (25, 50, 75 mg/L) for 24-48-72 h. tGSH, GSH, GSSG, and TBARS contents, GSH/GSSG ratio, and GPx, GR, GST and caspase enzyme activities were measured using spectrophotometrical methods, and Hsp70 content was measured by ELISA technique. The results demonstrated that spinosad exposure caused significant alterations in the GSH-related oxidative stress markers, and also caused increases in lipid peroxidation and stress proteins with inducing ROS generation in the liver. Apoptosis initiated with the induction of caspase-3 and Hsp70 could not protect the liver cells. Our results indicated that GSH-related antioxidant system tried to protect the liver cells from spinosad-induced hepatotoxicity however, the oxidative stress resulting from induction of ROS generation induced apoptosis in the liver of O. niloticus.

Organic insecticide spinosad causes in vivo oxidative effects in the brain of Oreochromis niloticus

Spinosad is an organic insecticide derived from a naturally occurring soil bacterium and is used in organic farming worldwide. The aim of this study was to evaluate in vivo toxic effects of spinosad in the brain of Oreochromis niloticus as a model organism. The fish were exposed to sublethal spinosad concentrations (25, 50, 75 mg L−1) for 24–48–72 h to determine tGSH, GSH, GSSG, and TBARS contents, GSH/GSSG ratio, and GPx, GR, GST enzymes activities using spectrophotometrical methods, and Hsp70 content by an ELISA technique. Spinosad caused elevations in the contents of tGSH, GSH, GSSG, Hsp70, and reductions in the ratio of GSH/GSSG and GPx activity and an induction in the GR activity. The results indicated that spinosad had oxidative effects in the brain tissue by altering the parameters in GSH‐related antioxidant system and Hsp70. It was also suggested that spinosad‐induced free‐radicals were eliminated by GSH‐related antioxidant system in the brain of Oreochromis niloticus.

Modulation of Fenthion-Induced Oxidative Effects by BSO in the Liver of Cyprinus carpio L

The objective of the present study was to evaluate the oxidative stress potential of low-level organophosphate fenthion exposure with the modulatory effect of buthionine sulfoximine in the liver of Cyprinus carpio L. The fish were exposed to 20% of 96-hour LC50 of fenthion for 24 and 96 hours. Total and oxidized glutathione, thiobarbituric acid reactive substances, protein levels, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, superoxide dismutase, and catalase-specific enzyme activities were measured spectrophotometrically. There was a 15-day depuration period to evaluate the changes in the studied parameters. Fenthion caused a time-dependent depletion of the total and reduced glutathione levels. The oxidized/reduced glutathione ratio and catalase specific enzyme activity were reduced while the glutathione-S-transferase activity was elevated. Intraperitonal buthionine sulfoximine application disclosed the inhibitory effect of fenthion on superoxide dismutase and glutathione peroxidase activities, whereas glutathione-S-transferase activity was increased. There was no change in lipid peroxidation levels during the experiments. No amelioration was observed in the affected parameters except the glutathione-S-transferase activity in the 15-day depuration period. In conclusion, glutathione-S-transferase and catalase enzyme activities and total and reduced glutathione levels were better estimators to monitor the effects of fenthion in low concentration in the liver of C. carpio. The depuration period was not adequate to recover the antioxidant capacity.

In vivo Alterations in Glutathione-Related Processes, Lipid Peroxidation, and Cholinesterase Enzyme Activities in the Liver of Diazinon-Exposed Oreochromis niloticus

ABSTRACT Although its usage is partially banned in developed countries, organophosphate (OP) pesticide diazinon finds extensive agricultural application in our country (Turkey). This study was conducted to evaluate the effects of diazinon on total glutathione (tGSH), GSH-related enzymes, cholinesterase (ChE) enzyme activities, and lipid peroxidation in the liver of Oreochromis niloticus, a freshwater fish, as a model organism. Fish were exposed to 0.1, 1, and 2 mg/L sublethal concentrations of diazinon for 1, 7, 15, and 30 days. Total GSH levels, GSH-related enzyme and ChE-specific activities, and malondialdehyde (MDA) levels were analyzed using spectrophotometric methods. tGSH levels are decreased at 1 day, while they were increased in the long-term period. GSH-related enzyme activities are affected by diazinon exposure, except glutathione reductase (GR; EC 1.6.2.4). Diazinon displayed an oxidative stress-inducing potential and it increased lipid peroxidation. Similar inhibition levels were observed in acetylcholinesterase (AChE; EC 3.1.1.7) and butyrylcholinesterase (BChE; EC 3.1.1.8.) enzyme activities, and these inhibitions were not dose dependent. ChE inhibition-related oxidative stress was observed using its correlation with elevated tGSH levels and increased glutathione S-transferase (GST; EC 2.5.1.18) enzyme activities; that reflects the diazinon-induced oxidative stress in the liver of O. niloticus. According to the results of the present study, tGSH level and GST-specific activity are suitable for reflecting the toxic effects of diazinon in fish.

In vivo acetylcholinesterase inhibition in the tissues of spinosad exposed Oreochromis niloticus

This study was conducted to evaluate the effects of an organic insecticide, spinosad on acetylcholinesterase (AChE, EC 3.1.1.7) specific activities in the brain and the liver tissues of juvenile Oreochromis niloticus, and also identify the indicator tissues in the fish. The fish were exposed to three sublethal spinosad concentrations (25, 50, 75mg/L) for 24-48-72h. Acetylcholinesterase activities were determined by spectrophotometrical methods. Acetylcholinesterase was significantly inhibited in both tissues tested. The inhibition percentages of AChE ranged for liver and brain tissues between 32-63% and 21-35%, respectively. The present study demonstrated that in vivo spinosad exposure caused AChE inhibition in the brain and the liver. The liver tissue might be suggested as an indicator tissue for spinosad exposure in the fish. Additional studies are needed to understand inhibition mechanisms of AChE by spinosad.

Neurotoxic effects of lambda‐cyhalothrin modulated by piperonyl butoxide in the brain of Oreochromis niloticus

The objective of this research was to investigate the neurotoxic effects of pyrethroid pesticide lambda‐cyhalothrin by the modulation of cytochrome P450 with piperonyl butoxide in the brain of juvenile Oreochromis niloticus. The fish were exposed to 0.48 μg L−1 (1/6 of the 96‐h LC50) lambda‐cyhalothrin and 10 μg L−1 piperonyl butoxide for 96 h and 15 days. tGSH, GSSG, TBARS contents, GPx, GR, GST, and AChE enzymes activities were determined by spectrophotometrical methods and Hsp70 content was analyzed by ELISA technique. Lambda‐cyhalothrin had no significant effect on the components of GSH redox system, lipid peroxidation and Hsp70 levels but inhibited AChE activity. In the presence of piperonyl butoxide, lambda‐cyhalothrin caused increases in tGSH, GSSG, TBARS and Hsp70 contents, GST activity, and decrease in AChE activity. Present results showed that in the presence of piperonyl butoxide, lambda‐cyhalothrin caused neurotoxic effects by increasing oxidative stress. Adaptation to its oxidative stress effects may be supplied by GSH‐related antioxidant system. Piperonyl butoxide revealed neurotoxic effect of lambda‐cyhalothrin.

Tissue-Specific In Vivo Inhibition of Cholinesterases by the Organophosphate Fenthion in Oreochromis niloticus

This study was designed to elucidate the effect of the organophosphate fenthion exposure on cholinesterase‐specific activities in brain, liver, and kidney tissues of juvenile Oreochromis niloticus, and to define the best indicator tissue to fenthion exposure. The 96‐h LC50 value was determined as 2.27 mg/L and fish were exposed to 20% of this concentration for 24‐, 48‐, and 96‐h. Recovery periods in similar durations were provided to evaluate the ChE activities. AChE and BChE activities were determined spectrophotometrically. The activities of these enzymes were significantly inhibited in all the tissues tested, liver was the most and kidney was the least affected tissues. The inhibition percentages of AChE and BChE were at similar levels in the liver while BChE was more affected in kidney. BChE was not detected in the brain. A significant positive correlation in ChE inhibitions was found among tissues, and the effect of fenthion on ChE activities was tissue specific. In general enzymes activities were not significantly recovered in 96‐h recovery period; however, an elevation in AChE inhibition was observed in brain. Based on the data of this study, the liver may be suggested as the best indicator tissue especially for phosphorothioate exposure.

Piperonyl butoxide increases oxidative toxicity of fenthion in the brain of Oreochromis niloticus.

The present study was designed to understand the effects of piperonyl butoxide (PBO), modulator of cytochrome P450 (CYP 450), on the neurotoxicity of organophosphate pesticide fenthion in the brain of Oreochromis niloticus used as a model organism. Fish were exposed to one‐fourth of the LC50 value of fenthion (0.567 mg/L) and 0.5 mg/L PBO concentration for 24 h, 96 h, and 15 days. Glutathione (GSH)‐related antioxidant system, lipid peroxidation, stress proteins, and acetylcholinesterase (AchE) activity were investigated. Our results showed that PBO induced the neurotoxic effect of fenthion with increasing oxidative stress in long‐term exposure. GSH‐related antioxidant system might take a role in protecting the brain from these oxidative effects. PBO possibly inhibited the biotransformation of fenthion by inhibiting CYP 450; thereby preventing the brain from AChE inhibition in short‐term exposure. Changes in parameters indicated that PBO caused biphasic response by affecting CYP 450 in the brain of O. niloticus.