Peter C. Melnyk
Illumina
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Publication
Featured researches published by Peter C. Melnyk.
ChemBioChem | 2007
Melissa D. Shults; Igor A. Kozlov; Nicholas A. Nelson; Bahram Ghaffarzadeh Kermani; Peter C. Melnyk; Veronika Shevchenko; Anu Srinivasan; Joseph Musmacker; John P. Hachmann; David L. Barker; Michal Lebl; Chanfeng Zhao
We report a novel protein kinase assay designed for high‐throughput detection of one or many kinases in a complex mixture. A solution‐phase phosphorylation reaction is performed on 900 different peptide substrates, each covalently linked to an oligonucleotide tag. After incubation, phosphoserine, phosphothreonine, and phosphotyrosine are chemically labeled, and the substrates are hybridized to a microarray with oligonucleotides complementary to the tags to read out the phosphorylation state of each peptide. Because protein kinases act on more than one peptide sequence, each kinase can be characterized by a unique signature of phosphorylation activity on multiple substrates. Using this method, we determined signatures for 26 purified kinases and demonstrated that enzyme mixtures can be screened for activity and selectivity of inhibition.
Nucleic Acids Research | 2012
Kerri York; Ryan C. Smith; Rob Yang; Peter C. Melnyk; Melissa Wiley; Casey Turk; Mostafa Ronaghi; Kevin L. Gunderson
We have developed a cost-effective, highly parallel method for purification and functionalization of 5′-labeled oligonucleotides. The approach is based on 5′-hexa-His phase tag purification, followed by exchange of the hexa-His tag for a functional group using reversible reaction chemistry. These methods are suitable for large-scale (micromole to millimole) production of oligonucleotides and are amenable to highly parallel processing of many oligonucleotides individually or in high complexity pools. Examples of the preparation of 5′-biotin, 95-mer, oligonucleotide pools of >40K complexity at micromole scale are shown. These pools are prepared in up to ~16% yield and 90–99% purity. Approaches for using this method in other applications are also discussed.
Combinatorial Chemistry & High Throughput Screening | 2008
Michal Lebl; Igor A. Kozlov; Peter C. Melnyk; John P. Hachmann; Anu Srinivasan; Melissa D. Shults; Chanfeng Zhao; Joseph Musmacker; Nicholas A. Nelson; David L. Barker
We have developed a miniaturized and multiplexed solution assay for the measurement of protease activity in complex samples. This technology can accelerate research in functional proteomics and enable biologists to carry out multiplexed protease inhibitor screens on a large scale. The assay readout is based on Illuminas universal Sentrix BeadArrays. The peptide sequences that serve as protease substrates are conjugated to oligonucleotide sequences complementary to the oligo tags on randomly assembled and decoded bead arrays. The peptide portion is C-terminally labeled with a biotin residue and contains a sequence of five histidine residues on the amino terminus. The unique oligonucleotide part of each oligonucleotide-peptide conjugate is attached to amino terminus of the peptide sequence. Upon protease cleavage, the biotin residue is cleaved from the oligonucleotide-peptide conjugate. Following the reaction, all biotin-containing species are captured and removed by incubation with streptavidin beads. The cleaved conjugates that remain in solution are captured by hybridization of their oligo sequence to Sentrix BeadArrays and detected using a labeled antibody against pentahistidine tag of the conjugate or by an antibody sandwich assay. We have generated multiple sets of oligonucleotide tagged peptide substrates of varying complexity (100 to 1000 substrates in a mixture) and show that the response of individual substrate is independent of the complexity of the mixture. Our initial results demonstrate the feasibility of assaying proteases in a multiplexed environment with high sensitivity.
Combinatorial Chemistry & High Throughput Screening | 2006
Igor A. Kozlov; Peter C. Melnyk; Chanfeng Zhao; John P. Hachmann; Veronika Shevchenko; Anu Srinivasan; David L. Barker; Michal Lebl
We have developed a high throughput assay for the measurement of protease activity in solution. This technology will accelerate research in functional proteomics and enable biologists to streamline protease substrate evaluation and optimization. The peptide sequences that serve as protease substrates in this assay are labeled on the carboxy terminus with a biotin moiety and a fluorescent tag is attached to the amino terminus. Protease cleavage causes the biotin containing fragment to be detached from the labeled peptide fragment. Following the protease treatment, all biotin containing species (uncleaved substrates and the cleaved carboxy terminal fragment of the substrate) are removed by incubation with streptavidin beads. The cleaved fluorescently labeled amino terminal part of the substrate remains in solution. The measured fluorescence intensity of the solution is directly proportional to the activity of the protease. This assay was validated using trypsin, chymotrypsin, caspase-3, subtilisin-A, enterokinase and tobacco etch virus protease.
Nucleosides, Nucleotides & Nucleic Acids | 2007
Igor A. Kozlov; Peter C. Melnyk; John P. Hachmann; David L. Barker; Michal Lebl; Chanfeng Zhao
We developed novel assays for high-throughput detection of one or many kinases or proteases. The assays use hundreds of different peptide substrates, each covalently linked to an oligonucleotide tag. After incubation with sample, the pool of substrates is hybridized to a microarray containing oligonucleotides complementary to the tag sequences. We screened several specific chemistries for the conjugation based on the following criteria: easy derivatization of oligonucleotides and peptides; high efficiency of the conjugation reaction; good stability of the conjugates; and satisfactory conjugate performance in our assays. We have validated selected method during the successful generation of thousands oligonucleotide-peptide conjugates.
Biopolymers | 2004
Igor A. Kozlov; Peter C. Melnyk; Katie Stromsborg; Mark S. Chee; David L. Barker; Chanfeng Zhao
Sensors and Actuators B-chemical | 2007
Bahram Ghaffarzadeh Kermani; Igor A. Kozlov; Peter C. Melnyk; Chanfeng Zhao; John P. Hachmann; David L. Barker; Michal Lebl
Archive | 2005
Igor A. Kozlov; Peter C. Melnyk
Archive | 2010
Igor A. Kozlov; Peter C. Melnyk; Chanfeng Zhao
Journal of Chromatographic Science | 2007
Igor A. Kozlov; Bahram Ghaffarzadeh Kermani; Peter C. Melnyk; David L. Barker; Chanfeng Zhao; John P. Hachmann; Michal Lebl