Peter D. Wilson
Simon Fraser University
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Peter D. Wilson.
ACS Chemical Biology | 2014
Elena V. Dolgosheina; Sunny Jeng; Shanker Shyam S. Panchapakesan; Razvan Cojocaru; Patrick S. K. Chen; Peter D. Wilson; Nancy Hawkins; Paul A. Wiggins; Peter J. Unrau
Because RNA lacks strong intrinsic fluorescence, it has proven challenging to track RNA molecules in real time. To address this problem and to allow the purification of fluorescently tagged RNA complexes, we have selected a high affinity RNA aptamer called RNA Mango. This aptamer binds a series of thiazole orange (fluorophore) derivatives with nanomolar affinity, while increasing fluorophore fluorescence by up to 1,100-fold. Visualization of RNA Mango by single-molecule fluorescence microscopy, together with injection and imaging of RNA Mango/fluorophore complex in C. elegans gonads demonstrates the potential for live-cell RNA imaging with this system. By inserting RNA Mango into a stem loop of the bacterial 6S RNA and biotinylating the fluorophore, we demonstrate that the aptamer can be used to simultaneously fluorescently label and purify biologically important RNAs. The high affinity and fluorescent properties of RNA Mango are therefore expected to simplify the study of RNA complexes.
Organic and Biomolecular Chemistry | 2006
Michael P. A. Lyle; Peter D. Wilson
The evaluation of a chiral, nonracemic and C2-symmetric 2,2-bipyridyl ligand in copper(I)-catalyzed asymmetric allylic oxidation reactions of a series of cyclic alkenes with tert-butyl peroxybenzoate is reported (up to 91% ee, the highest reported enantioselectivity for a bipyridyl ligand copper(I) complex to date).
Organic and Biomolecular Chemistry | 2006
Michael P. A. Lyle; Neil D. Draper; Peter D. Wilson
The synthesis of a series of chiral nonracemic and C2-symmetric 2,2-bipyridyl ligands (R = Me, i-Pr and Ph) as well as the syntheses of the corresponding unsymmetric 2,2-bipyridyl ligands (R = Me and Ph) is described. These bipyridyl ligands were prepared, in a notably direct and modular fashion, from the readily available and corresponding 2-chloropyridine acetals (R = Me, i-Pr and Ph). The bipyridyl ligands were evaluated in copper(I)-catalyzed cyclopropanation reactions of styrene with the ethyl and t-butyl esters of diazoacetic acid. The stereoselectivities, as well as the yields of the cyclopropanation reactions, were dependant on the ratio of the bipyridyl ligands and copper triflate that was employed. The best result was obtained in the asymmetric cyclopropanation reaction of styrene and tert-butyl diazoacetate with the C2-symmetric bipyridyl ligand (R = i-Pr). This afforded the corresponding trans-cyclopropane in good diastereoselectivity (4 : 1) and in moderate enantioselectivity (44% ee). The X-ray structure determination of a complex formed between the C2-symmetric 2,2-bipyridyl ligand (R = Ph) and copper(I) chloride showed that two bipyridyl ligands had coordinated to the copper(I) ion. This information, along with the results of a series of cyclopropanation reactions and NMR data, led to the conclusion that the 2,2-bipyridyl ligands had the propensity to form catalytically inactive bis-ligated copper(I) species in solution that were in equilibrium with catalytically active copper(i) triflate and the desired mono-ligated copper(I) species. Moreover, it was observed that the complex of the bipyridyl ligand (R = Ph) and copper(I) chloride had a particularly large optical rotation (sodium D-line). The maximum positive optical rotation was subsequently found to be +1.1 x 10(4) at 304 nm and the maximum negative optical rotation was -1.3 x 10(4) at 329 nm.
Organic Letters | 2005
Michael P. A. Lyle; Neil D. Draper; Peter D. Wilson
Organic Letters | 2005
Jeremy D. Pettigrew; Jay A. Cadieux; Simon S. S. So; Peter D. Wilson
Organic Letters | 2004
Michael P. A. Lyle; Peter D. Wilson
Journal of Organic Chemistry | 2004
Hongjuan Hu; Tyler J. Harrison; Peter D. Wilson
Organic Letters | 2006
Jeremy D. Pettigrew; Peter D. Wilson
Canadian Journal of Chemistry | 2004
Jeremy D. Pettigrew; Rebecca P. Freeman; Peter D. Wilson
Journal of Organic Chemistry | 2006
Jeremy D. Pettigrew; Peter D. Wilson