Peter de Jong

A fast scanner for fluorescence microscopy using a 2-D CCD and time delayed integration

We have developed an imaging system for high speed image acquisition in fluorescence microscopy. The use of a two- dimensional CCD array in a special operation mode called TDI (Time Delayed Integration) permits a significant increase in photon integration time compared to 1D scanners (higher signal-to-noise ratio) without compromising the total data throughput rate. Instead of a start–stop system we use continuous stage motion in the CCDs parallel shift direction. Synchronizing the parallel clock and the stage velocity guarantees a one-to-one relationship between a moving cell and its image onto the CCD. Compared to start-stop system, TDI scanning offers a speed improvement, negligible blurring in the scanning direction and a complete suppression of pixel variability boosting the SNR more than 10 dB.

Adaptable office buildings

Purpose – Across use adaptation is a possible way of dealing with long term vacant office buildings, albeit previous research shows that there are many obstacles to be thrived. In The Netherlands several successful transformations of offices into housing were completed. Nevertheless, transformations do not take place on a large scale. High building costs are the main reason. Hence, new office developments should anticipate future programmatic transformation. This paper seeks answers to the questions: Is it possible to anticipate future programmatic change? To what extent will anticipation on future possibilities influence building costs?Design/methodology/approach – The authors reviewed existing studies to gather information about transformation building costs and the critical success factors of transformations. The building design and costs for new office buildings were then studied, designed with enhanced transformation potential, focussing on two standard office building types, the central core tower a...