Peter Skrabal

27Al, 15N, 13C and 1H NMR spectra of the 2:1 aluminium(III) complexes of some azo dyes

The 27Al, 15N, 13C and 1H NMR spectra in DMSO and 27Al and 15N magic angle spinning NMR spectra of 2:1 aluminium(III) complexes derived from 5‐chloro‐2‐hydroxyaniline azo coupling products with acetoacetanilide, 3‐methyl‐1‐phenylpyrazol‐5‐one and 2‐naphthol were measured and analysed. It was found that the aluminium atom is six‐coordinated, being bound to two oxygens and the nitrogen originating from 2‐hydroxy‐5‐chloroaniline of each ligand, in an analogous fashion to 2:1 cobalt(III) complexes described earlier.

Metabolic flux analysis of hybridoma cells using mass balances, CO2 mass spectrometry and NMR spectrometry.

The analysis of the intracellular metabolic fluxes in mammalian cells can be used for optimizing culture media, for tracing metabolic bottle-necks and for toxicological and metabolic studies. It is desirable to determine metabolic fluxes without using isotopic tracers. This could potentially be achieved by using the material balances of the relevant metabolites. For each metabolite a mass balance is set up using the known biochemical stoichiometry and its measured uptake or production rate. Together, these mass balances form a linear set of equations, containing the unknown fluxes. However, such a set of linear equations is underdeterwined due to the presence of cyclic pathways in mammalian cell metabolism. Fluxes in such pathways have to be quantitated using isotopic tracers.