Petr Marvan

Morphological differences in natural populations of the genus botryococcus chlorophyceae

Forty seven populations of Botryococcus-like algae collected all over the world were studied with respect to the variability of their morphological features. A considerable heterogeneity of our material, reflected in pronounced differences in cell shape, cell size, morphology of mucilaginous sheaths as well as in intracellular structures (presence of pyrenoids), was found, which suggests the existence of several separate species. Statistical and numerical methods were therefore applied with the aim to support their delimitation by more objective arguments. Thirteen morphological types were recognized, to which the species status can be ascribed according to the criteria used in taxonomy of coccal green algae. The new species Botryococcus comperei, B. australis, B. fernandoi, B. terribilis and B. pila were defined. The possibility and usefulness of establishing more taxa distinguishable at higher than specific level are discussed.

A review of the alien and expansive species of freshwater cyanobacteria and algae in the Czech Republic

The invasion and spread of non-native species of many different kinds of organisms is of increasing interest to researchers. Invasions by microscopic organisms, however, are poorly understood, and their impact on the environment is probably underestimated. We collected available data on non-native and invasive/expansive algae and cyanobacteria in the Czech Republic; we mapped their distribution and assessed their impact on local species and other real or potential risks resulting from their spread. The list of alien species in the Czech Republic contains 10 species of cyanobacteria, 9 species of Bacillariophyceae, 1 species of Dinophyta, 1 species of Ulvophyceae, 2 species of Chlorophyceae, and 1 species complex of Zygnematopyceae. The literature on the worldwide occurrence of these taxa is also reviewed.

Identification versus counting protocols as sources of uncertainty in diatom-based ecological status assessments

In 2009, seventeen analysts participated in a pan-European diatom ring-test (intercalibration), analyzing nine samples from seven countries following the European standard EN 14407. The objective of this exercise was to agree on practical conventions on diatom identification to facilitate future intercalibration work and to assess the extent to which national differences in sample analysis (counting protocol and identification conventions) contribute to variability in EU-level comparisons of diatom-based methods. Differences in the reported taxa lists were large, but not a major source of variation in values of a common metric (the phytobenthos Intercalibration Common Metric, ICM). Therefore, every country can apply its own identification conventions for national assessments, and still be fairly confident that the ICM reflects the national classification of its streams. Part of the index variation was due to differences in counting protocols and care should be taken when handling broken valves, girdle views and small taxa. More work at both national and European level is needed to provide a harmonized way of using diatoms for ecological status assessments in the future.

Diatoms in water quality assessment: to count or not to count them?

Abstract Two methods of quantifying diatoms were compared: (i) by counting valves, and (ii) by simple assignment of individual species to a seven-point scale according to their coverage on a glass slide. The saprobity-trophic index introduced in the Czech Republic for assessing the ecological status of running waters in accordance with the requirements of the Water Framework Directive (WFD) was used for comparison. There were no statistically significant differences between the indices as calculated by the studied methods, and therefore the comparable indicative values of the two approaches were demonstrated. In all cases, however, indices based on coverage classes achieved higher correlation with the fundamental water chemistry parameters than did indices based on diatom valve counts. To explain the cause of higher correlations, different variants of editing input data were used. The results support the assumption that the main cause of the poorer predictive value for the index based on diatom valve counts is that the abundant taxa are attributed too much importance in the final assessment of the environment quality. Using degrees of coverage for the quantification of diatoms in evaluating the ecological status thus appears to be preferable (or at least sufficient) for the given purpose.