Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Petra Horáková is active.

Publication


Featured researches published by Petra Horáková.


Chemistry: A European Journal | 2011

Alkylsulfanylphenyl Derivatives of Cytosine and 7‐Deazaadenine Nucleosides, Nucleotides and Nucleoside Triphosphates: Synthesis, Polymerase Incorporation to DNA and Electrochemical Study

Hana Macíčková-Cahová; Radek Pohl; Petra Horáková; Luděk Havran; Jan Špaček; Miroslav Fojta; Michal Hocek

Aqueous Suzuki-Miyaura cross-coupling reactions of halogenated nucleosides, nucleotides and nucleoside triphosphates derived from 5-iodocytosine and 7-iodo-7-deazaadenine with methyl-, benzyl- and tritylsufanylphenylboronic acids gave the corresponding alkylsulfanylphenyl derivatives of nucleosides and nucleotides. The modified nucleoside triphosphates were incorporated into DNA by primer extension by using Vent(exo-) polymerase. The electrochemical behaviour of the alkylsulfanylphenyl nucleosides indicated formation of compact layers on the electrode. Modified nucleotides and DNA with incorporated benzyl- or tritylsulfanylphenyl moieties produced signals in [Co(NH(3))(6)](3+) ammonium buffer, attributed to the Brdička catalytic response, depending on the negative potential applied. Repeated constant current chronopotentiometric scans in this medium showed increased Brdička catalytic response, which suggests the deprotection of the alkylsulfanyl derivatives to free thiols under the conditions.


Analytical Chemistry | 2010

Direct voltammetric analysis of DNA modified with enzymatically incorporated 7-deazapurines.

Hana Pivoňková; Petra Horáková; Miloslava Fojtová; Miroslav Fojta

Nucleic acids studies use 7-deazaguanine (G*) and 7-deazaadenine (A*) as analogues of natural purine bases incapable of forming Hoogsteen base pairs, which prevents them from being involved in DNA triplexes and tetraplexes. Reduced propensity of the G*- and/or A*-modified DNA to form alternative DNA structures is utilized, for example, in PCR amplification of guanine-rich sequences. Both G* and A* exhibit significantly lower potentials of their oxidation, compared to the respective natural nucleobases. At carbon electrodes, A* yields an oxidation peak which is by about 200-250 mV less positive than the peak due to adenine, but coincides with oxidation peak produced by natural guanine residues. On the other hand, oxidation signal of G* occurs at a potential by about 300 mV less positive than the peak due to guanine, being well separated from electrochemical signals of any natural DNA component. We show that enzymatic incorporation of G* and A* can easily be monitored by simple ex situ voltammetric analysis of the modified DNA at carbon electrodes. Particularly G* is shown as an attractive electroactive marker for DNA, efficiently incorporable by PCR. While densely G*-modified DNA fragments exhibit strong quenching of fluorescence of SYBR dyes, commonly used as fluorescent indicators in both gel staining and real time PCR applications, the electrochemical detection provides G*-specific signal suitable for the quantitation of the amplified DNA as well as for the determination of the DNA modification extent. Determination of DNA amplicons based on the measurement of peak G*(ox) is not affected by signals produced by residual oligonucleotide primers or primary templates containing natural purines.


Analytical Chemistry | 2010

Determination of the level of DNA modification with cisplatin by catalytic hydrogen evolution at mercury-based electrodes.

Petra Horáková; Lucie Těsnohlídková; Ludek Havran; Pavlína Vidláková; Hana Pivonkova; Miroslav Fojta

Electrochemical methods proved useful as simple and inexpensive tools for the analysis of natural as well as chemically modified nucleic acids. In particular, covalently attached metal-containing groups usually render the DNA well-pronounced electrochemical activity related to redox processes of the metal moieties, which can in some cases be coupled to catalytic hydrogen evolution at mercury or some types of amalgam electrodes. In this paper we used voltammetry at the mercury-based electrodes for the monitoring of DNA modification with cis-diamminedichloroplatinum (cisplatin), a representative of metallodrugs used in the treatment of various types of cancer or being developed for such purpose. In cyclic voltammetry at the mercury electrode, the cisplatin-modified DNA yielded catalytic currents the intensity of which reflected DNA modification extent. In square-wave voltammetry, during anodic polarization after prereduction of the cisplatinated DNA, a well-developed, symmetrical signal (peak P) was obtained. Intensity of the peak P linearly responded to the extent of DNA modification at levels relevant for biochemical studies (rb = 0.01-0.10, where rb is the number of platinum atoms bound per DNA nucleotide). We demonstrate a correlation between the peak P intensity and a loss of sequence-specific DNA binding by tumor suppressor protein p53, as well as blockage of DNA digestion by a restriction endonuclease Msp I (both caused by the DNA cisplatination). Application of the electrochemical technique in studies of DNA reactivity with various anticancer platinum compounds, as well as for an easy determination of the extent of DNA platination in studies of its biochemical effects, is discussed.


Monatshefte Fur Chemie | 2015

Enzyme-linked electrochemical detection of DNA fragments amplified by PCR in the presence of a biotinylated deoxynucleoside triphosphate using disposable pencil graphite electrodes

Lucia Hároníková; Jan Špaček; Medard Plucnara; Petra Horáková; Hana Pivoňková; Luděk Havran; Arzum Erdem; Miroslav Fojta

In this report, we present a simple electrochemical detection protocol for the detection of specific PCR-amplified DNA fragments, based on incorporation of biotin tags into DNA amplicons during PCR run in the presence of a biotinylated nucleoside triphosphate. For detection, an enzyme-linked electrochemical system involving streptavidin–alkaline phosphatase conjugate attached to the biotinylated DNA, adsorbed at the surface of a disposable pencil graphite electrode, is used. The enzyme converts an inactive indicator, 1-naphthyl phosphate, into electrochemically oxidizable indicator 1-naphthol that is subsequently detected. Excellent selectivity of this fast, facile, and inexpensive analysis not requiring any sophisticated electrode modification and its applicability for off-line monitoring of DNA amplification is demonstrated. Applications of the technique include detection of the presence of specific nucleotide sequences in biological samples, such as sequences related to pathogenic microorganism or transgenes.Graphical abstract


Nucleic acids symposium series (2004) | 2008

Novel base-functionalized DNA. Efficient methodology for construction and bioanalytical applications

Michal Hocek; Milan Vrabel; Hana Cahová; Jan Riedl; Lubica Kalachova; Hana Pivoňková; Petra Horáková; Luděk Havran; Miroslav Fojta

A novel efficient two-step methodology for the construction of base-functionalized DNA is based on direct aqueous cross-coupling reactions of unprotected nucleoside triphosphates followed by polymerase incorporation. Preliminary applications of the modified DNA in electrochemical detection and bioanalysis are outlined.


Chemistry: A European Journal | 2009

Base-modified DNA labeled by [Ru(bpy)(3)](2+) and [Os(bpy)(3)](2+) complexes: construction by polymerase incorporation of modified nucleoside triphosphates, electrochemical and luminescent properties, and applications.

Milan Vrabel; Petra Horáková; Hana Pivoňková; Lubica Kalachova; Hana Černocká; Hana Cahová; Radek Pohl; Peter Šebest; Luděk Havran; Michal Hocek; Miroslav Fojta


Chemistry: A European Journal | 2011

Anthraquinone as a redox label for DNA: synthesis, enzymatic incorporation, and electrochemistry of anthraquinone-modified nucleosides, nucleotides, and DNA.

Jana Balintová; Radek Pohl; Petra Horáková; Pavlína Vidláková; Luděk Havran; Miroslav Fojta; Michal Hocek


Organic and Biomolecular Chemistry | 2011

Tail-labelling of DNA probes using modified deoxynucleotide triphosphates and terminal deoxynucleotidyl tranferase. Application in electrochemical DNA hybridization and protein-DNA binding assays

Petra Horáková; Hana Macíčková-Cahová; Hana Pivoňková; Jan Špaček; Luděk Havran; Michal Hocek; Miroslav Fojta


Analytical Chemistry | 2008

Osmium tetroxide, 2,2'-bipyridine: electroactive marker for probing accessibility of tryptophan residues in proteins.

Miroslav Fojta; Sabina Billová; Ludek Havran; Hana Pivonkova; Hana Černocká; Petra Horáková; Emil Paleček


Current Analytical Chemistry | 2011

Osmium Tetroxide Complexes as Versatile Tools for Structure Probing and Electrochemical Analysis of Biopolymers

Miroslav Fojta; Pavel Kostečka; Hana Pivonkova; Petra Horáková; Ludek Havran

Collaboration


Dive into the Petra Horáková's collaboration.

Top Co-Authors

Avatar

Miroslav Fojta

Central European Institute of Technology

View shared research outputs
Top Co-Authors

Avatar

Hana Pivoňková

Academy of Sciences of the Czech Republic

View shared research outputs
Top Co-Authors

Avatar

Luděk Havran

Academy of Sciences of the Czech Republic

View shared research outputs
Top Co-Authors

Avatar

Michal Hocek

Charles University in Prague

View shared research outputs
Top Co-Authors

Avatar

Radek Pohl

Academy of Sciences of the Czech Republic

View shared research outputs
Top Co-Authors

Avatar

Hana Macíčková-Cahová

Academy of Sciences of the Czech Republic

View shared research outputs
Top Co-Authors

Avatar

Hana Pivonkova

Academy of Sciences of the Czech Republic

View shared research outputs
Top Co-Authors

Avatar

Ludek Havran

Academy of Sciences of the Czech Republic

View shared research outputs
Top Co-Authors

Avatar

Jan Špaček

Academy of Sciences of the Czech Republic

View shared research outputs
Top Co-Authors

Avatar

Medard Plucnara

Academy of Sciences of the Czech Republic

View shared research outputs
Researchain Logo
Decentralizing Knowledge