Pierre A. Fortier

Activity of interneurons within the L4 spinal segment of the cat during brainstem-evoked fictive locomotion

SummaryExtracellular recordings from interneurons located in the L4 spinal segment were made during fictive locomotion produced by electrical stimulation of the mesencephalic locomotor region (MLR) in the paralysed decerebrate cat. Only interneurons within the L4 segment which received group II input from quadriceps, sartorius or the pretibial flexor muscle afferents and which had axonal projections to motor nuclei in L7 were selected for analysis. During the fictive step cycle two thirds of these interneurons fired action potentials during the time of activity in the ipsilateral hindlimb flexor neurograms. These cells were also less responsive to peripheral input during the extension phase of the fictive locomotion cycle. The remaining one third of the interneurons examined were not rhythmically active during locomotion. The possible contributions of the midlumbar interneurons to motoneuron activity during locomotion are discussed.

Locomotor deficits in the mutant mouse, Lurcher.

SummaryThe effect of total Purkinje cell degeneration on treadmill locomotion was studied in the cerebellar mutant mouse Lurcher. Other movements such as swimming and scratchting were also studied in order to evaluate the cerebellar control of rhythmic actions. Cinematographic and electromyographic recordings were taken from normal and Lurcher mice that were subsequently perfused to obtain a Purkinje cell count. Walking deteriorated progressively and was clearly abnormal in 30 day old Lurchers with 90% Purkinje cell degeneration. In adult Lurcher mice in which Purkinje cells were totally absent, walking was characterized by short steps with exaggerated hindlimb flexion in the swing phase. Also, both the interlimb step ratio, defined as the step length of the reference limb divided by the step length of the opposite limb, and the interlimb coupling, defined as the temporal relation of one footfall with respect to the footfall of another limb, varied more than in normal mice. Furthermore, the locomotion of Lurcher mice displayed increased vertical displacement of the hip and an inability to produce continuous step cycles without stumbling. Both the EMG onset relative to foot contact and the EMG burst duration were highly variable, and a greater overlap in the activities of antagonist muscles at the transition from ankle extension to flexion was evident. Although both walking and swimming involve cyclical limb movements, the disorganization of the cycle and the irregular EMG pattern seen in the Lurcher during walking were not observed during swimming. Furthermore, scratching was well executed in the Lurcher mice. However, a consistently higher tonic extensor activity at the ankle appeared during walking, swimming and scratching. These results suggest that, in contrast to swimming and scratching, the requirements of walking depend to a greater degree on a functional cerebellar cortex for successful performance.

Field potential mapping of neurons in the lumbar spinal cord activated following stimulation of the mesencephalic locomotor region

The spinal neurons involved in the control of locomotion in mammals have not been identified, and a major step that is necessary for this purpose is to determine where these cells are likely to be located. The principal objective of this study was to localize lumbar spinal interneurons activated by stimulation of the mesencephalic locomotor region (MLR) of the cat. For this purpose, extracellular recordings of MLR-evoked cord dorsum and intraspinal field potentials were obtained from the lumbosacral enlargement during fictive locomotion in the precollicular-postmammillary decerebrate cat preparation. Potentials recorded from the dorsal surface of the cord between the third lumbar (L3) and first sacral (S1) segments typically showed four short-latency positive waves (P1-P4). These P-waves were largest between the L4-L6 segments. The amplitude of the P2–4 waves increased with the appearance of locomotion and displayed rhythmic modulation during the locomotor step cycle. Microelectrode recordings from the L4-L7 spinal segments during fictive locomotion revealed the presence of both positive and negative short-latency MLR-evoked intraspinal field potentials, and were used to construct isopotential maps of the evoked potentials. Positive field potentials were observed throughout the dorsal horn of the L4-L7 spinal segments with the largest amplitude potentials occurring in laminae III-VI. Negative field potentials were found in laminae VI-X of the lumbar cord. The shortest latency negative field potentials were observed in lamina VII and at the border between laminae VI and VII and were considered to be evoked monosynaptically from the arrival of the descending volley. Short-latency mono- and disynaptic negative field potentials were also observed in lamina VIII. Longer latency, tri- and polysynaptic field potentials were observed in laminae VII and VIII. Many of the longer latency negative waves observed in laminae VII and VIII followed shorter latency negative potentials recorded from the same location. Laminae VII and VIII negative field potentials were largest in the L5–6 and L4–5 spinal segments, respectively. Negative field potentials were also evoked in the motor nuclei of the L4–7 spinal segments. The segmental latencies for these potentials indicate that they were evoked di- and trisynaptically.(ABSTRACT TRUNCATED AT 400 WORDS)

Quantitative analyses of neurons projecting to primary motor cortex zones controlling limb movements in the rat

The objective was to determine if projections of single neurons to primary motor cortex preferentially terminate in several efferent zones that could form synergies for the execution of limb movements. Intracortical microstimulation was used to identify zones evoking hip flexion (HF), elbow flexion (EF), and both plantarflexion (PF) and dorsiflexion (DF) about the ankle. Histological examination showed that the zones from which some movements were evoked extended beyond the agranular cortex into granular cortex. Fluorogold, Fast blue, and propridium iodide or rhodamine-labeled dextran were injected into three of these four efferent zones in each rat. There was a virtual absence of multiple-labeled cells despite having an intermingling of different-colored cells of which 15% in frontal cortex were less than 1.2 mm away from a neighboring neuron that projected to a different efferent zone. This suggests that single neurons projecting to the motor cortex do not hard-wire specific synergies but rather project to single efferent zones in order to offer the greatest degree of freedom for the generation of movements. The distribution of ventral posterolateral and ventrolateral thalamic nucleus labeling depended on whether the injections were in granular or agranular cortex. Conversely, frontal cortex projections to motor efferent zones were made irrespective of their location in either granular or agranular cortex and thereby supporting their presumed role in the control of movements. Hindlimb motor cortex injections yielded retrograde labeling that extended into the more localised distribution of frontal cortex neurons retrogradely labeled from forelimb injections. This may allow hindlimb movements to be synchronized by forelimb movements during walking on challenging terrain.

Early enhancement but no late changes of motor responses induced by intracortical microstimulation in the ketamine-anesthetized rat.

The objectives of this study were to determine whether changes in electromyographic (EMG) responses observed during prolonged intracortical microstimulation (ICMS) were due to local plasticity of the motor system or to global changes in the preparation. Local effects would be expressed as changes only along the activated motor pathway, whereas global effects would be expressed as changes also appearing at distant cortical efferent microzones. The results of ICMS in the ketamine-anesthetized rat showed that the size of consecutive EMG responses increased gradually to a relatively stable magnitude over a period of four to six trains of stimuli. This early enhancement of EMG responses was maintained while continuously providing trains of stimuli at 1 Hz. However, it disappeared after a 5-min period of muscle inactivity. This response enhancement in the presence of ketamine (an NMDA, N-methyl-d-aspartate, receptor blocker) suggests that a neuronal mechanism involving non-NMDA-mediated homosynaptic short-term potentiation (STP) was responsible for the early enhancement of EMG responses. To compare ICMS effects at several time intervals it was necessary to average several evoked EMG responses because there was normal biological variability between single EMG responses. To determine the optimal number of EMG responses that would provide a reliable average EMG response, averages of 5, 10, 15, 20, and 25 EMG responses evoked from a single cortical site were collected at 5-min intervals. The results revealed that averages of 10 responses would provide reliable average EMG responses for all subsequent analyses. There were wide fluctuations in the average EMG responses when periodic injections of ketamine were used to maintain a low reflexive state in the animal. Switching to continuous infusion of ketamine abolished these fluctuations but there remained a small drift in the magnitudes of consecutive EMG responses. To test whether this drift reflected local plastic changes in the motor system induced by stimulation or some global changes, EMG responses evoked from another ICMS site were used as control. The rationale was that global effects would affect all motor output sites equally. The sizes of control EMG responses followed a similar time course to those evoked from the test site. Furthermore, standardizing the test EMG responses with respect to the control responses eliminated the drift in response magnitudes. Thus the drift was due to slow global changes in neuronal excitability possibly produced by the anesthesia. In conclusion, late changes occurring after hours of ICMS were not due to plasticity of the motor system but rather to global changes in the preparation, possibly resulting from the inability to set an ideal anesthetic infusion rate that could maintain a constant level of neuronal excitability over long periods of time. However, there was early enhancement of the EMG responses evoked by ICMS due to neuronal plasticity possibly mediated by a non-NMDA mechanism of homosynaptic STP such as post-tetanic potentiation (PTP). This early enhancement would favor recruitment of the previously activated motor pathway and lead to greater consistency in movement execution.

The Impact of a Pre-Vocational Program on Cognition, Symptoms, and Work Re-Integration in Schizophrenia

This study measured the effects of a pre-vocational program on cognition, symptoms, and integration to work in schizophrenia. Twelve participants, receiving pharmacological treatment only, were compared to 14 participants, who were part of a pre-vocational program and who received pharmacological treatment on pre and post measures. The work skills training group was offered by an occupational therapist. Results showed statistically significant differences in negative and general symptoms, visual attention, learning, and integration to work when comparing the two groups in favor of the program. These results provide evidence of the positive effects of occupational therapy interventions in mental health.

Postsecondary academic achievement and first-episode psychosis: A mixed-methods study.

Background. Postsecondary academic achievement as an area of functional recovery for young adults living with mental illness has received little research attention. Purpose. This study had three purposes: to compare rates of participation, performance, and satisfaction in postsecondary education between young adults with first-episode psychosis and closely matched young adults; to identify characteristics associated with academic participation; and to explore the processes associated with educational experiences. Method. One hundred young adults ages 18 to 30 participated in the study. Quantitative data on academic engagement, performance, and satisfaction, and qualitative data (n = 52) on academic experiences were integrated through pattern analyzes. Findings. Young adults with psychosis were significantly less likely to be engaged in postsecondary education. No difference appeared for the extent of engagement, but performance and satisfaction were lower among participants with psychosis. Participants engaged in reflexive decision making to access postsecondary education and to maintain adequate academic performance. Strategies used by successful students with mental illness were identified. Implications. Assessment and intervention focused on educational needs and skills should become landmark practices for psychiatric rehabilitation practitioners, including occupational therapists.

Detecting and estimating rectification of gap junction conductance based on simulations of dual-cell recordings from a pair and a network of coupled cells

Gap junctions can exhibit rectification of conductance. Some reports use inequality of coupling coefficients as the first sign of the possible existence of rectification (Devor and Yarom, 2002; Fan et al., 2005; Levavi-Sivan et al., 2005; Mann-Metzer and Yarom, 1999; Nolan et al., 1999; Szabadics et al., 2001). However, mathematical modeling and simulations of electrotonic coupling between an isolated pair of neurons showed conditions where the coupling coefficients were unreliable indicators of rectification. On the other hand, the transfer resistances were found to be reliable indicators of junctional rectification. The existing mathematical model of cell coupling (Bennett, 1966; Devor and Yarom, 2002; Verselis and Veenstra, 2000) was extended in order to measure rectification of the junctional conductances directly between dual-recorded neurons whether isolated or surrounded by a simulated 3-dimensional network of heterogeneous cells whose gap junctions offered parallel paths for current flow between the recorded neurons. The results showed that the transfer resistances could still detect rectification of the gap junction linking the dual-recorded neurons when embedded in a coupled cell network and that a mathematical model could estimate the conductances in both directions through this gap junction using only data that would be available from real dual-intracellular penetrations which allow electrophysiological recordings and intracellular staining. Rectification of gap junctions in unrecorded cells of a biologically realistic coupled cell network had negligible effects on the voltage responses of the dual-recorded neurons because of minimal current passing through these surrounding cells.

Perception of Community Functioning in Young Adults with Recent-Onset Psychosis: Implications for Practice

Introduction: Early and intensive rehabilitation for individuals living with first-episode psychosis (FEP) is a key issue for occupational therapists working in mental health settings. Purpose: The purpose of this multiple case study was to explore the perception of outpatients in a youth psychosis clinic in Montreal regarding their community functioning. Method: Nineteen young adults with FEP were interviewed, using a set of questions developed on the basis of a person-environment relationship theoretical model. The steps of data analysis included the transcription and coding of data, data reduction, the aggregation of themes and visual representation. Findings: The participants experienced more handicap-creating situations than competence situations. The themes included a diminished quality of relationship with parents, social isolation and difficulties in work and academic performance, as well as poor access to education. The perception of the participants on each of these themes is elaborated. Practice implications: Specific intervention targets for young adults with FEP should include issues such as the management of energy level, communication and social skills training, residential stability, academic and work rehabilitation and attention to physical features of the home, school and work environments.

Transitions to adulthood in first-episode psychosis: a comparative study

The first aim of this study is to compare attainment of five markers of transition to adulthood between young adults with first‐episode psychosis and young adults without any psychopathology. The second aim is to examine if the association between age group and transition to adulthood is similar for individuals who experience first‐episode psychosis versus those who do not.