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Publication
Featured researches published by Pierre Becker.
BMC Microbiology | 2017
Anne-Cécile Normand; Carole Cassagne; Magali Gautier; Pierre Becker; Stéphane Ranque; Marijke Hendrickx; Renaud Piarroux
BackgroundSeveral Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry protocols, which differ in identification criteria, have been developed for mold and dermatophyte identification. Currently, the most widely used approach is Bruker technology, although no consensus concerning the log(score) threshold has been established. Furthermore, it remains unknown how far increasing the number of spots to compare results might improve identification performance.In this study, we used in-house and Bruker reference databases as well as a panel of 422 isolates belonging to 126 species to test various thresholds. Ten distinct identification algorithms requiring one to four spots were tested.ResultsOur findings indicate that optimal results were obtained by applying a decisional algorithm in which only the highest score of four spots was taken into account with a 1.7 log(score) threshold. Testing the entire panel enabled identification of 87.41% (in-house database) and 35.15% (Bruker database) of isolates, with a positive predictive value (PPV) of 1 at the genus level for both databases as well as 0.89 PPV (in-house database) and 0.72 PPV (Bruker database) at the species level. Applying the same rules to the isolates for which the species were represented by at least three strains in the database enabled identification of 92.1% (in-house database) and 46.6% (Bruker database) of isolates, with 1 PPV at the genus level for both databases as well as 0.95 PPV (in-house database) and 0.93 PPV (Bruker database) at the species level.ConclusionsDepositing four spots per extract and lowering the threshold to 1.7, a threshold which is notably lower than that recommended for bacterial identification, decreased the number of unidentified specimens without altering the reliability of the accepted results. Nevertheless, regardless of the criteria used for mold and dermatophyte identification, commercial databases require optimization.
Mycoses | 2018
Berdieke Goemaere; Pierre Becker; Eric Van Wijngaerden; Johan Maertens; Isabel Spriet; Marijke Hendrickx; Katrien Lagrou
Candidaemia is an important health problem in immunocompromised patients with an epidemiology varying with region, period and patient population involved. The occurrence of candidaemia and the associated species distribution over a 12‐year period at a large tertiary care centre in Belgium were analysed. The trend in incidence in the intensive care units (ICUs) and non‐ICUs was investigated as well as the influence of antifungal exposure on the species distribution. From 2004 until 2015, 865 candidaemia episodes occurred in 826 patients at the University Hospitals Leuven. Candida albicans (59%) remained the most important cause of candidaemia, followed by C. glabrata (22.4%) and C. parapsilosis (8%). The mean incidence in the whole hospital was 1.48 per 10 000 patient days (PD). The incidence in ICUs increased reaching up to 10.7 per 10 000 PD whereas in the non‐ICUs, the incidence decreased. Prior exposure to fluconazole and echinocandins was associated with candidaemia caused by less susceptible species. Candidaemia incidence increased in the whole hospital, driven by ICUs. Surveillance of candidaemia epidemiology on a local scale is of high value to guide empirical treatment strategies.
Medical Mycology | 2014
Pierre Becker; Annelies De Bel; Delphine Martiny; Stéphane Ranque; Renaud Piarroux; Carole Cassagne; Monique Detandt; Marijke Hendrickx
Mycoscience | 2015
Pierre Becker; Dirk Stubbe; Jessie Claessens; Sam Roesems; Yves Bastin; Chantal Planard; Carole Cassagne; Renaud Piarroux; Marijke Hendrickx
Antimicrobial Agents and Chemotherapy | 2018
Berdieke Goemaere; Katrien Lagrou; Isabel Spriet; Marijke Hendrickx; Pierre Becker
Journal De Mycologie Medicale | 2017
Elizabet D’hooge; Pierre Becker; Dirk Stubbe; Renaud Piarroux; Marijke Hendrickx
Journal De Mycologie Medicale | 2017
Anne-Cécile Normand; Carole Cassagne; Lilia Hasseine; M. Gari-Toussaint; Frédéric Gabriel; Isabelle Accoceberry; Damien Costa; Nathalie Bourgeois; Sophie Cassaing; Cécile Nabet; Christine Bonnal; Hélène Raberin; Markus Stein; Ignace Surmont; Denis Piérard; Farid Djenad; Jean-Luc Donnadieu; Martine Piarroux; Stéphane Ranque; Pierre Becker; Marijke Hendrickx; Renaud Piarroux
Archive | 2016
Pierre Becker; Philippe Szternfeld; M Andjelkovic; Roel Anthonissen; Luc Verschaeve; Marijke Hendrickx; Marine Renard; Yannick Lara; Benoit Durieu; Véronique Simons; H Dail Laughinghouse; Luc Cornet; Denis Baurain; Annick Wilmotte
Journal De Mycologie Medicale | 2016
Anne-Cécile Normand; Farid Djenad; Pierre Becker; Frédéric Gabriel; M. Gary-Toussaint; Carole Cassagne; M. Gautier; Stéphane Ranque; Isabelle Accoceberry; P. Marty; Marijke Hendrickx; Renaud Piarroux
Archive | 2015
Berdieke Goemaere; Katrien Lagrou; M Goossens; K Latour; B Catry; Marijke Hendrickx; Pierre Becker