Pierre Labadie
University of Sussex
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Featured researches published by Pierre Labadie.
Steroids | 2007
Pierre Labadie; Mika Peck; Christophe Minier; Elizabeth M. Hill
Vertebrate-type sex steroids have been detected in a number of mollusk species and may play a role in the reproductive physiology of the animal. Mollusks are also exposed to exogenous estrogenic steroids that are present in sewage effluents, and these may add to the estrogenic burden of exposed animals. We investigated the uptake of estrogens in the blue mussel, Mytlius edulis and report for the first time the identity of estrogen fatty acid ester metabolites formed in vivo in an invertebrate. We exposed mussels to waterborne radiolabeled [(14)C]-17beta-estradiol (E2) or estrone (E1) and determined the nature of their metabolites using radio-HPLC and mass spectrometry (MS). After 13 days of exposure to 10ng/L E2, concentrations of radiolabeled residues were 2428-fold higher in M. edulis soft tissues compared with the ambient water concentration of E2. All the E2 residues in the mussel were present as a lipophilic ester which, in depuration studies, had a half-life of 8.3 days. Exposure of mussels to [(14)C]-E1 (70ng/L) resulted in formation of a similar lipophilic metabolite that after hydrolysis released [(14)C]-E2. Tandem MSMS analyses of the purified steroid ester fraction isolated from mussels exposed to either E2 or E1 revealed that they had the same composition and comprised C16:0, C16:1 and C16:2 esters of E2. This work reveals that in vivo E1 is rapidly metabolized to E2 in mussels prior to conjugation to C16 fatty acid esters, proving that C17-ketoreductase and C16 fatty acid acyl-CoA:E2 acyltransferase are important enzymes for the metabolism of estrogens in M. edulis.
Marine Environmental Research | 2012
Elena Cubero-Leon; A. Mirel Puinean; Pierre Labadie; Corina M. Ciocan; Naoki Itoh; Mitsuyo Kishida; Makoto Osada; Christophe Minier; Elizabeth M. Hill; Jeanette M. Rotchell
Members of the vertebrate CYP3A subfamily are involved in the metabolism of steroids and a wide range of xenobiotics. In this study two CYP3A-like mRNAs have been isolated from the mussel (Mytilus edulis), and their seasonal expression profile and modulation by estrogens examined. Sexual dimorphism of CYP3A-like mRNA expression was not observed in mussel gonads of individuals collected throughout a year. Nevertheless, natural variation in gonadal CYP3A-like mRNA expression was observed, with highest levels of CYP3A isoform1 and lowest levels of CYP3A isoform2 mRNA during the maturation and spawning season. Exposure to a 10% sewage treatment works extract did not result in any significant changes in mRNA expression of CYP3A-like. In contrast, exposure to E2 (200xa0ng/L) and TBT (100xa0ng/L) significantly down-regulated the expression of CYP3A-like isoform1 but not CYP3A-like isoform2 suggesting differential regulation.
Journal of Chromatography A | 2007
Pierre Labadie; Elizabeth M. Hill
Aquatic Toxicology | 2006
A.M. Puinean; Pierre Labadie; Elizabeth M. Hill; Makoto Osada; Mitsuyo Kishida; R. Nakao; Apolonia Novillo; I.P. Callard; Jeanette M. Rotchell
Chemosphere | 2007
Mika Peck; Pierre Labadie; Christophe Minier; Elizabeth M. Hill
14th IWA/IAHR International Conference on Urban Drainage (ICUD) | 2017
Steven Deshayes; Johnny Gasperi; Emilie Caupos; Pierre Labadie; Hélène Budzinski; Sophie Ayrault; Pascale Neveu; Paupardin Julien; Flanagan Kelsey; Lucie Varnede; Marie-Christine Gromaire
Archive | 2008
Marie-Jeanne Teil; Fabrice Alliot; H. Blanchard; Catherine Bourges; C. Dargnat; Annie Desportes; Johnny Gasperi; Pierre Labadie; Catherine Lorgeoux; Régis Moilleron; Elodie Moreau-Guigon; K. Tlili; Marc Chevreuil
River Basins - International Conference on Monitoring, Modelling and Management of River Basins | 2017
David Gateuille; Johnny Gasperi; Elodie Moreau-Guigon; Fabrice Alliot; Blanchard Martine; Chevreuil Marc; Marie-Jeanne Teil; Jean-Marc Brignon; Pierre Labadie; Hélène Budzinski; Vincent Rocher; Sam Azimi; Daniel Thevenot; Régis Moilleron; Michel Meybeck; Jean-Marie Mouchel
Colloque PIREN-Seine, 2017 | 2017
Jean-Marie Mouchel; Sophie Ayrault; Hélène Budzinski; Cédric Fisson; Nicolas Flipo; Josette Garnier; Johnny Gasperi; Sophie Guillon; Angélique Goffin; Pierre Labadie; Legall Marion; Alexia Marescaux; Michel Meybeck; Gilles Varrault; Shuaitao Wang; Aline Cattan
Novatech 2016, 28 juin - 1er juillet 2016, Lyon, France (FRA) | 2016
Marie-Christine Gromaire; A Bak; Ph Branchu; Adèle Bressy; S. Bruzzone; Hélène Budzinski; E. Caupos; De Gouvello; Jf Deroubaix; Steven Deshayes; Ph Dubois; Hélène Herlichman; Kelsey Flanagan; Johnny Gasperi; Pierre Georgel; Tala Kanso; Pierre Labadie; Laurent Meffray; Régis Moilleron; Ch Neaud; Pascale Neveu; Julien Paupardin; David Ramier; Tina Ratovelomanana; M. Saad; Martin Seidl; Eric Thomas; Jean-Yves Viau