Pierre Waffo Teguo

Trans-resveratrol-3-O-β-glucoside (piceid) in cell suspension cultures of vitis vinifera

Suspension cultures of Vitis vinifera were found to produce catechins and stilbenes. When the cells were grown in a polyphenol-inducing medium, a stilbene glucoside (piceid) was isolated. By using spectroscopic methods, the structure was determined as trans-resveratrol-3-O-β-glucoside.

Production of 13C-labelled anthocyanins by Vitis vinifera cell suspension cultures

The use of plant cell cultures for producing isotopically (13C) labelled phenolic substances is reported. Vitis vinifera cells synthesize high levels of anthocyanins when they are cultured in a polyphenol synthesis-inducing medium. Three major anthocyanin monoglucosides found in red wine were identified in grape cells: cyanidin-3-O-beta-glucoside, peonidin-3-O-beta-glucoside, and malvidin-3-O-beta-glucoside. Kinetic study of the intracellular level of phenylalanine and its metabolites showed that it is preferable to add this precursor to grape cell suspensions after the 5th day of culture, i.e. at the beginning of the exponential growth phase. After adding phenylalanine to the culture medium, its uptake was complete and the accumulation of anthocyanins in grape cells was stimulated. Incorporation of [1-13C]-phenylalanine into anthocyanins was measured by means of 13C satellites in the proton NMR spectrum. The maximal rate of 13C enrichment anthocyanins obtained with this technique reached 65%. The production of 13C labelled phenolic compounds was undertaken in order to investigate their absorption and metabolism in humans.

First evidence of trans-resveratrol production in cell suspension cultures of cotton (Gossypium hirsutum L.)

For the first time, trans-resveratrol, a stilbene, has been identified in cotton cell suspensions. Cell suspensions of Coker 312, a cultivar which produces embryogenic structures, acccumulate trans-resveratrol contrary to those of cultivar R405-2000, which do not. This stilbene may be a good phenolic marker for induction of somatic embryogenesis in cotton.

Polyphenol levels in two cotton (Gossypium hirsutum L.) callus cultures

Abstract We study the production of polyphenols by callus cultures from two cultivars of cotton (Gossypium hirsutum L.). Coker 312 produces the best calli and has embryogenic potential contrary to that of R405-2000, which is non- embryogenic. There is a relationship between the level of phenolic compounds and the callus cultures. Callogenesis in Coker 312 is highly correlated to a higher content of catechin, caffeic acid, ferulic acid, rutin, o—coumaric acid and salicylic acid. On the other hand, the cinnamic acid derivates (trans-cinnamic, cis and trans—methoxycinnamic acids) found in high amounts in R405-2000 calli could have a negative influence on the induction of cotton callogenesis.

Inhibition de la lipoperoxydation des LDL humaines par des stilbènes nouvellement isolés de cellules de Vigne (Vitis vinifera)

Inhibition de la lipoperoxydation des LDL humaines par des stilbenes nouvellement isoles de cellules de Vigne (Vitis vinifera) Pierre Waffo Teguo a , Bernard Fauconneau a , Alain Decendit a , Francois Huguet a , Gerard Deffieux a & Jean-Michel Merillon a a UFR Pharmacie, Universite Victor Segalen Bordeaux II, Laboratoire de Mycologie et Biotechnologie Vegetale , 3 place de la Victoire, F-33000 , Bordeaux Published online: 27 Apr 2013.