Pijush Mallick

Glucan of a somatic hybrid mushroom, pfls1h: structural characterization and study of immunological activities

A water-soluble glucan (PS-I) was isolated from the aqueous extract of the fruit bodies of a hybrid mushroom, pfls1h of Pleurotus florida and Lentinus squarrosulus (Mont.) Singer. Structural characterization of PS-I was carried out using total hydrolysis, methylation analysis, periodate oxidation, and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HSQC, and HMBC). Methylation analysis revealed that PS-I was composed of (1→3, 6), (1→3), (1→6)-linked and terminal β-d-glucopyranosyl residues in a relative proportion of approximately 1:1:1:1. The repeating unit of the glucan consists of a backbone chain of two (1→6)-β-d-glucopyranosyl residues, one of which is branched at O-3 position with (1→3)-β-d-glucopyranosyl and terminated with a β-d-glucopyranosyl residue. Study of immunological activity revealed that PS-I stimulates the splenocytes, thymocytes and macrophages.

Structural characterization and study of immunoenhancing properties of a glucan isolated from a hybrid mushroom of Pleurotus florida and Lentinula edodes

A water soluble glucan isolated from hot aqueous extract of fruit bodies of an edible hybrid mushroom Pfle1r of Pleurotus florida and Lentinula edodes showed macrophages, splenocytes, and thymocytes activation. The glucan consists of terminal, (1→3,6)-linked, and (1→6)-linked β-D-glucopyranosyl moieties in a molar ratio of nearly 1:1:3. On the basis of acid hydrolysis, methylation, periodate oxidation study, and NMR studies ((1)H, (13)C, DEPT-135, TOCSY, DQF-COSY, NOESY, ROESY, HSQC, and HMBC), the structure of the repeating unit of the glucan was established as: [structure: see text].

A partially methylated mannogalactan from hybrid mushroom pfle 1p: purification, structural characterization, and study of immunoactivation

A new water-soluble heteropolysaccharide (PS-II) with apparent molecular weight ∼1.65×10(5) Da, was isolated from the fruiting bodies of hybrid mushroom pfle 1p by hot aqueous extraction. It is composed of d-mannose, d-galactose, and 3-O-Me-d-galactose in a molar ratio of 1.0:0.99:1.1. The structural investigation of PS-II has been carried out using acid hydrolysis, methylation analysis, periodate oxidation study, and 1D/2D NMR experiments. Based on the results of these experiments, it was established that PS-II contained a main chain of (1→6) linked α-d-galactopyranosyl residues, one of which was substituted at C-2 by a terminal mannopyranosyl residue and also methylated at C-3 position. This heteropolysaccharide (PS-II) exhibited macrophage activation by NO production as well as in vitro splenocyte and thymocyte stimulation.

A heteropolysaccharide from an edible hybrid mushroom pfle 1p: structural and immunostimulating studies

A water-soluble heteropolysaccharide (PS-I) having molecular weight ~2.1×10(5) Da was isolated from hot aqueous extract of the fruit bodies of hybrid mushroom pfle 1p. The hybrid mushroom pfle 1p was obtained through intergenic protoplast fusion between Pleurotus florida and Lentinula edodes. The heteropolysaccharide contained D-glucose, D-galactose, and D-mannose in a molar ratio of nearly 4:2:1. The structural investigation of PS-I has been carried out using sugar and methylation analyses as well as 1D/2D NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HSQC, and HMBC). Based on the results of these experiments, the repeating unit of the PS-I was established as: [structure: see text]. PS-I showed in vitro macrophage activation by NO production and also stimulated splenocytes and thymocytes.

Structural characterization of an immunoenhancing heteroglycan of a hybrid mushroom (pfls1h) of Pleurotus florida and Lentinus squarrosulus (Mont.) Singer

An immunostimulating water-soluble heteroglycan (PS-II) was isolated from an aqueous extract of the fruit bodies of a hybrid mushroom, pfls1h produced by intergeneric protoplast fusion between Pleurotus florida and Lentinus squarrosulus (Mont.) Singer. Structural characterization of PS-II was carried out using sugar analysis, methylation experiment, periodate oxidation and 1D/2D NMR studies. Sugar analysis indicated the presence of glucose, mannose and galactose in a molar ratio of 1:1:2. Methylation analysis revealed that PS-II was composed of (1→6)- and (1→2,4,6)-α-D-galactopyranosyl, terminal β-D-mannopyranosyl and terminal β-D-glucopyranosyl residues in a relative proportion of approximately 1:1:1:1. On the basis of chemical analysis and NMR studies, the structure of the repeating unit of the heteroglycan was established to consist of a backbone chain of two (1→6)-α-D-galactopyranosyl residues, one of which is branched at O-2 with terminal β-D-Manp and at O-4 with terminal β-D-Glcp. This heteroglycan (PS-II) showed in vitro splenocyte, thymocyte and macrophage activations.

Genome instability in fruit body derived lines generated from fruiting pfle somatic hybrid lines and development of hybrid strain specific SCAR marker in edible mushroom

Abstract Six fruit body derived lines (pfle FB) generated from six fruiting pfle somatic hybrid mushroom lines showed genetic diversity analysed by fruit body morphology and inter simple sequence repeat (ISSR) markers. Stipe length, pelius diameter and bioefficiency % (BE%) of all the strains showed variations between each other with respect to Pleurotus florida parent. Hybrid pfle 1v and pfle 1q showed the highest value of stipe length and pelius diameter, respectively, compared with parent P. florida. Four ISSR primers amplified a total of 47 reproducible fragments with 82.9% polymorphism in which primer ISSR-03 produced the highest number of amplicons. Unweighted pair group method with arithmetic mean (UPGMA) based dendrogram exhibited two major groups in which hybrids pfle 1r and pfle 1q showed genetical closeness to parents P. florida and Lentinula edodes, respectively. Tissue culture generated line from fruit body of pfle 1r hybrid showed maximum BE% compared with the other hybrids and P. florida parent. For identification of this line, a pair of hybrid strain-specific SCAR marker (RFB2F and RFB2R) was developed based on an unique 813 bp RAPD amplicon.