Píndaro Álvarez-Ruiz

Detection of white spot syndrome virus in filtered shrimp-farm water fractions and experimental evaluation of its infectivity in Penaeus (Litopenaeus) vannamei.

Detection of white spot syndrome virus in filtered shrimp-farm water fractions and experimental evaluation of its infectivity in Penaeus (Litopenaeus) vannamei Héctor M. Esparza-Leal , César M. Escobedo-Bonilla , Ramón Casillas-Hernández , Píndaro Álvarez-Ruíz , Guillermo Portillo-Clark , Roberto C. Valerio-García , Jorge Hernández-López , Jesús Méndez-Lozano , Norberto Vibanco-Pérez , Francisco J. Magallón-Barajas d,⁎

Detection of white spot syndrome virus (WSSV) in the Pacific oyster Crassostrea gigas.

Oysters Crassostrea gigas were placed at water supply canals of three shrimp farms in Guasave, Mexico where WSSV outbreaks occur. Animals were sampled through April-August and September-December to detect WSSV DNA. By using three different PCR protocols, only oysters from a farm undergoing a WSSV outbreak were found WSSV-positive in gills and digestive gland. Two WSSV amplicons were sequenced and they corresponded over 99% to WSSV genome segments. Results showed that oysters can capture WSSV particles suspended in water. Susceptibility of oysters to WSSV infection and their role as a carrier remain to be determined.

Use of organic and inorganic fertilization in zero-discharge tanks and ponds and its effects on plankton and shrimp Litopenaeus vannamei performance

The aim of this study was to investigate the effects of organic (molasses) and inorganic (Nutrilake®) fertilization on plankton, water quality, and shrimp Litopenaeus vannamei performance reared in zero-discharge tanks and ponds. The findings showed that in tanks, the organic fertilization induced highest survival and production, and low total ammonia-N concentration; however, this effect was not observed in the ponds. In both tanks and ponds, the inorganic fertilization increased nitrogen levels. The phytoplankton abundance increased in both tanks/ponds with the fertilization treatments. Shrimp production in the tanks and ponds fertilized almost doubled compared with unfertilized treatments.