Piotr Gomułka

Reproductive parameters of common carp (Cyprinus carpio L) spawners during natural season and out-of-season spawning

Common carp spawning characteristics were compared between season and out-of-season periods. There were no differences in the percentage of ovulation, spermatozoa motility and embryo survival to the eyed-egg-stage between the spawning periods. Fish spawned during the season produced oocytes of higher total weight than those spawned out-of-season. On the day exogenous feeding began, larvae obtained in out-of-season spawning were shorter than those obtained during the spawning season. However, the out-of-season larvae started feeding two days earlier than in-season larvae. As a result, seven days after fertilization, larvae obtained during out-of-season had the same length as larvae obtained during season. It appears to be possible to obtain common carp larvae half a year before the natural spawning period without decreasing the quality of gametes and larvae.

Haematological and Biochemical Blood Profile in Russian Sturgeon Following Propofol and Eugenol Anaesthesia

Sturgeon production involves unavoidable handling procedures inducing stress response. Clove oil is the anaesthetic commonly used in sturgeon culture to minimise stress and pain. Propofol is one of the most frequently used anaesthetic in humans. Propofol is a promising candidate anaesthetic for fish. The aim of the study was to compare the effect of propofol and eugenol on haematological and biochemical blood profile of Russian sturgeon. Determined indices covered: PCV, RBC, Hb, MCV, MCHC, MCH, leucogram, and concentration of calcium cations, inorganic phosphates, total proteins, albumins, globulins, ammonia, triacylglycerols, glucose, and the activity of creatine kinase, alkaline phosphatase and aspartate aminotransferase. Exposure to both anaesthetics caused a stress response in Russian sturgeon expressed as changes in WBC system and increased values of PCV, Hb, glucose, triacylglycerols, inorganic phosphates, calcium and ammonia. The impact of eugenol was more sever and longer being. No damage of internal organs was stated. According to our results, propofol is safe anaesthetic for Russian sturgeon.

Selection of Extraction Method for the Estimation of the Bioaccumulation Factor of 4-N-Nonylphenol and 4-Tert-Octylphenol in an Aquatic System

Abstract A few extraction methods for the reverse-phase high performance liquid chromatography (RP-HPLC) determination of alkylphenolic compounds in fish tissues were examined. The best results of the isolation of 4-nonylphenol (4-n-NP) and 4-tert-octylphenol (4-t-OP) from Rainbow trout were obtained using soxhlet extraction; the recoveries reached 64.64 ± 7.26% and 65.26 ± 5.29%, respectively. The subsequent solid phase extraction (SPE) cleanup process of fish extract was optimized using four different sorbents and the highest recoveries of the analytes were obtained for aminopropyl silica cartridges. Their biological concentration factors (BCF) expressing the ratio between the concentration in Rainbow trout and in water were determined for two temperatures, 15 and 20°C.

Effects of propofol and carbon dioxide on acid-base balance in Siberian sturgeon

The aim of this study was to compare physiological responses in Siberian sturgeon (Acipenser baeri) induced by propofol and CO2 anaesthesia. Two procedures were applied during the experiment. In procedure I, blood samples were collected immediately after exposure (1, 2, 5, 10 min) to the anaesthetic. In procedure II, fish were exposed to the anaesthetic for 10 minutes and then were moved to anaesthetic free water. Blood was sampled after 5, 10, 20 or 30 min of recovery time. Gasometrical and biochemical analyses were performed on collected blood. In CO2 anaesthetized fish strong hypercapnic acidosis was revealed. The drop of the HCO3-/CO2 ratio, from 28:1 in control fish up to 4:1 in CO2 anaesthetized ones, proved that the compensation mechanism is not capable of preventing acidosis during CO2 anaesthesia in Siberian sturgeon. In contrast, only moderate, respiratory acidosis occurred in sturgeons anaesthetised with propofol. Hypercapnic acidosis during CO2 anaesthesia was followed by a fourfold increase of ammonia level in the blood. Glucose level, increasing only during recovery time, indicates that a secondary stress response occurred when awareness of anaesthetized fish had been restored.