Piotr Oleskowicz-Popiel

The future of anaerobic digestion and biogas utilization

One of the common tendencies of animal production activities in Europe and in developed countries in general is to intensify the animal production and to increase the size of the animal production units. High livestock density is always accompanied by production of a surplus of animal manure, representing a considerable pollution threat for the environment in these areas. Avoiding over-fertilization is not only important for environmental protection reasons but also for economical reasons. Intensive animal production areas need therefore suitable manure management, aiming to export and to redistribute the excess of nutrients from manure and to optimize their recycling. Anaerobic digestion of animal manure and slurries offers several benefits by improving their fertilizer qualities, reducing odors and pathogens and producing a renewable fuel - the biogas. The EU policies concerning renewable energy systems (RES) have set forward a fixed goal of supplying 20% of the European energy demands from RES by year 2020. A major part of the renewable energy will originate from European farming and forestry. At least 25% of all bioenergy in the future can originate from biogas, produced from wet organic materials such as: animal manure, whole crop silages, wet food and feed wastes, etc.

The challenge of enzyme cost in the production of lignocellulosic biofuels.

With the aim of understanding the contribution of enzymes to the cost of lignocellulosic biofuels, we constructed a techno-economic model for the production of fungal cellulases. We found that the cost of producing enzymes was much higher than that commonly assumed in the literature. For example, the cost contribution of enzymes to ethanol produced by the conversion of corn stover was found to be

Near infrared and acoustic chemometrics monitoring of volatile fatty acids and dry matter during co-digestion of manure and maize silage.

0.68/gal if the sugars in the biomass could be converted at maximum theoretical yields, and

Microwave-assisted conversion of microcrystalline cellulose to 5-hydroxymethylfurfural catalyzed by ionic liquids

1.47/gal if the yields were based on saccharification and fermentation yields that have been previously reported in the scientific literature. We performed a sensitivity analysis to study the effect of feedstock prices and fermentation times on the cost contribution of enzymes to ethanol price. We conclude that a significant effort is still required to lower the contribution of enzymes to biofuel production costs.

Effect of pH and retention time on volatile fatty acids production during mixed culture fermentation

In this study, two process analytical technologies, near infrared spectroscopy and acoustic chemometrics, were investigated as means of monitoring a maize silage spiked biogas process. A reactor recirculation loop which enables sampling concomitant with on-line near infrared characterisation was applied. Near infrared models resulted in multivariate models for total and volatile solids with ratio of standard error of performance to standard deviation (RPD) values of 5 and 5.1, indicating good on-line monitoring prospects. The volatile fatty acid models had slopes between 0.83 and 0.92 (good accuracy) and RPD between 2.8 and 3.6 (acceptable precision). A second experiment employed at-line monitoring with both near infrared spectroscopy and acoustic chemometrics. A larger calibration span was obtained for total solids by spiking. Both process analytical modalities were validated with respect to the total solids prediction. The near infrared model had an RPD equal to 5.7, while the acoustic chemometrics model resulted in a RPD of 2.6.

Liquid hot water pretreatment on different parts of cotton stalk to facilitate ethanol production

Ionic liquid (IL) has been widely investigated in 5-HMF production from biomass. However, most of studies employed IL as reaction solvent which requires a large amount of IL. In the present study, IL was utilized as catalyst in the conversion of microcrystalline cellulose (MCC) to 5-HMF under microwave irradiation (MI) in N,N-dimethylacetamide (DMAc) containing LiCl. 1,1,3,3-tetramethylguanidine (TMG)-based ILs, including 1,1,3,3-tetramethylguanidine tetrafluoroborate ([TMG][BF4]) and 1,1,3,3-tetramethylguanidine lactate ([TMG]L) which were commonly used in the absorption of SO2 and CO2 from flue gas, were synthesized and applied in the conversion of MCC to 5-HMF for the first time. Of the catalysts employed, [TMG]BF4 showed high catalytic activity in 5-HMF production from MCC. The condition including the ratio of IL to MCC, temperature and time for MCC conversion was optimized using Central Composite Design (CCD) and Response Surface Methodology (RSM). The highest 5-HMF yield of 28.63% was achieved with the optimal condition.

Lignocellulosic ethanol production without enzymes – Technoeconomic analysis of ionic liquid pretreatment followed by acidolysis

Mixed culture fermentation consists of stable microbial population hence waste could be potentially used as a substrates. The aim of the work was to investigate the impact of pH and retention time on the anaerobic mixed culture fermentation. Trials at different pH (4-12) in unbuffered systems were conducted for 5, 10 and 15days. The highest VFAs concentration was achieved after 15days at pH 10 (0.62g/gVSadded), promising results were also achieved for pH 11 (0.54g/gVSadded). For pH 4 and short retention time propionic acid was the major product instead of acetic acid. For batches run at 15days (besides pH 6) caproic acid presence was noticed whereas at pH 11 occurrence of succinic was quantified. Significant correlation between operational factors and fermentations effluents was proved. Throughout changing simple operating parameters one could design process to produce desirable concentration and composition of VFAs.

One-pot conversion of disaccharide into 5-hydroxymethylfurfural catalyzed by imidazole ionic liquid

To investigate pretreatment demand for different parts of biomass, cotton stalk was separated into stem, branch and boll shell, which were treated by liquid hot water pretreatment (LHWP) with severity from 2.77 to 4.42. Based on weight loss (WL, w/w) mainly caused by hemicellulose removal, it was found that boll shell (WL, 46.93%) was more sensitive to LHWP than stem (WL, 38.85%). Although ethanol yield of 18.3, 16.27 and 21.08g/100g was achieved from stem, branch and boll shell with pretreatment severity at 4.42, ratio of ethanol yield to pretreatment energy input for particular parts was different. For boll shell and branch, the maximum ratio of ethanol yield to energy input were 1.37 and 1.33g ethanolkJ(-1) with severity at 4.34, while it was 1.20 for stem at 3.66. This indicates that different pretreatment demands for different parts of plants should be considered in order to save pretreatment energy input.

A Simulation Model of Combined Biogas, Bioethanol and Protein Fodder Co-Production in Organic Farming

Deconstruction of polysaccharides into fermentable sugars remains the key challenge in the production of inexpensive lignocellulosic biofuels. Typically, costly enzymatic saccharification of the pretreated biomass is used to depolymerize its cellulosic content into fermentable monomers. In this work, we examined the production of lignocellulosic recovery, a process that does not require the use of enzymes to produce fermentable sugars. In the base case, the minimum ethanol selling price (MESP) was

Treatment of different parts of corn stover for high yield and lower polydispersity lignin extraction with high-boiling alkaline solvent

8.05/gal, but with improved performance of the hydrolysis, extraction, and sugar recovery, the MESP can be lowered to