Polyanna Nunes Herculano

Colagenase de pescada branca: extração, purificação parcial, caracterização e teste de especificidade ao colágeno para aplicação industrial

1Laboratory of Technology of Bioactive Product (LABTECBIO). Department of Morphology and Animal Physiology, DMFA, Federal Rural University of Pernambuco UFRPE, Av. Dom Manoel de Medeiros, s/n, 52171-900, Recife, PE; vagne_melo@hotmail.com (corresponding author) 2Laboratory of Enzymology (LABENZ). Department of Biochemistry, Federal University of Pernambuco-UFPE, Campus Universitário, s / n, 50670-901, Recife, PE ABSTRACT

Complexo celulolítico produzido por duas espécies de fungos fitopatogênicos isolados de mandioca

Material collected from commercial crops of cassava (Manihot esculenta Crantz) in 11 municipalities in the state of Paraiba included 27 isolates of the fungi Colletotrichum gloeosporoides f. sp. manihotis Henn (Penn.) and Fusarium oxysporum Schlent., which are pathogenic to the culture. From these isolates, cellulolytic enzyme complex production was obtained under submerged fermentation at 120 rpm and 30 °C for 7 days, with CMC (Carboxymethyl Cellulose) as carbon source. Enzyme activities were quantified by means of the degree of hydrolysis of strips of filter paper (FPAse) and CMC (CMCase). The release of Paranitrofenol was used to quantify the activities of β-glucosidase (βG). The highest activities were: 23.13 and 1.51 U/ml for FPase and CMCase by the isolates of C. gloeosporioides URM 7124 and 7125. For βG, 0.0014 U/ml by isolate 13.