Pooja Moteriya

Synthesis and characterization of silver nanoparticles using Caesalpinia pulcherrima flower extract and assessment of their in vitro antimicrobial, antioxidant, cytotoxic, and genotoxic activities

Abstract Caesalpinia pulcherrima flower extract mediated synthesis of silver nanoparticles was attempted in the present work including optimization of some procedure parameters. Characterization of synthesized silver nanoparticles was done by various spectral analyses. The size of synthesized silver nanoparticles was 12 nm and they were spherical in shape. The green synthesized silver nanoparticles were further evaluated for antimicrobial, antioxidant, cytotoxic, and genotoxic activities; they showed good antimicrobial, antioxidant, and cytotoxic effects. Genotoxic study revealed non-toxic nature at lower concentration. Overall results suggest that the synthesized silver nanoparticles have pronounced applicability in pharmaceutical and biomedical field.

Characterization, synergistic antibacterial and free radical scavenging efficacy of silver nanoparticles synthesized using Cassia roxburghii leaf extract

In this work, we report for the first time, synthesis of silver nanoparticles using Cassia roxburghii leaf extract and evaluate its synergistic antibacterial efficacy and antioxidant potential. The synergistic antimicrobial activity of silver nanoparticles with commercial antibiotics against Gram-positive, Gram-negative bacteria and fungi was evaluated. The antioxidant potential of synthesized silver nanoparticles was evaluated by FRAP and ABTS radical scavenging antioxidant assays. UV–Vis spectra peak at 473 and color change from colorless to brown color confirmed the formation of AgNPs. X-ray diffraction (XRD) and selected area electron diffraction (SAED) pattern confirmed the crystalline nature of synthesized nanoparticles. Transmission electron microscopy (TEM) analysis revealed the average size of particles to be 15–20 nm. Fourier transform infrared spectroscopy (FTIR) revealed the functional groups C. roxburghii leaf that was responsible for the reduction and capping process of nanoparticles. C. roxburghii leaf extract synthesized AgNPs showed good synergistic antimicrobial against Gram-negative bacteria even better than some of the antibiotics used as positive control; they also showed antioxidant potential. The results suggest that the synthesized nanoparticles can be used to treat multidrug resistant microorganisms and stress related diseases and disorders.

Biosynthesis of silver nanoparticles formation from Caesalpinia pulcherrima stem metabolites and their broad spectrum biological activities

The present work illustrates eco-friendly, rapid and cost effective method of AgNPs synthesis using C. pulcherrima stem extract. Initially, various physico chemical factors were optimized. Characterization was done by different spectroscopic and microscopic analysis. AgNPs were spherical in shape with an average size of 8 nm. AgNPs showed good synergistic antimicrobial, antibiofilm and antioxidant activity. The cytotoxicity effect against HeLa cancer cell line was dose dependent while genotoxic study revealed the non toxic nature of AgNPs at lower concentration. The results suggest that AgNPs from C. pulcherrima stem extract have great potential in biomedical applications.

Quinoline Derivatives: In Vitro Antimicrobial Study

Some quinoline derivatives were synthesized and their structures were confirmed by IR, 1H NMR and mass spectroscopy. Screening of all these synthesized compounds were done in vitro against four Gram positive bacteria, four Gram negative bacteria and four fungal strains in dimethyl sulphoxide and N, N-dimethyl formamide. It is observed that N, N-dimethyl formamide is good solvent for the these compounds in selected strains and compounds containing cyano and nitro groups are more effective in inhibiting these strains. *Corresponding author: Shipra Baluja, Department of Chemistry, Saurashtra University, Rajkot, 360005, Gujarat, India, E-mail: shipra_baluja@rediffmail.com Citation: Baluja, S., et al. Quinoline Derivatives: In Vitro Antimicrobial Study. (2017) J Pharm Pharmaceutics 4(1): 105110. Quinoline Derivatives: In Vitro Antimicrobial Study Shipra Baluja1*, Sumitra Chanda2, Pooja Moteriya2, Paras Ramavat1 Received date: April 04, 2016 Accepted date: January 10, 2017 Published date: January 18, 2017 DOI: 10.15436/2377-1313.17.017 Baluja, S., et al. Out of various classes of hetercycles, Quinolines have attracted much attention, because a large number of natural and synthetic products possess this heterocyclic unit[11-13]. Literature survey shows that various quinoline derivatives are known to possess a wide range of pharmacological properties, such as antiviral[14], antitubercular[15], antidiabetic[16], antibacterial[17], anticancer[18], antiarthritic, analgesic[19] antiinflammatory[20], antioxidant[21] etc. Owing to their interesting biological properties, in the present work some novel quinoline derivatives were synthesized from arylidine, dimidone and ammonium acetate. The characterization of these synthesized compounds was done by IR, 1H NMR and mass spectral analysis. The antimicrobial activity of the synthesized compounds was done against some pathogenic Gram positive and Gram negative bacteria and fungi in dimethyl sulphoxide and N, N-dimethyl formamide.