Pragati Patel

Effect of Ascorbic Acid on mRNA Expression of HSP70 Gene in WLH Egg Type Growers During Heat Stress

Meteorological factors such as high ambient temperature and high relative humidity exert adverse effects on poultry production. Heat stress results in poor growth performance, feed efficiency, egg production and higher mortality. The aim of the experiment was to explore and quantify the relative mRNA expression of HSP70 gene in relation to ascorbic acid supplementation in White Leghorn (WLH) egg type growers exposed to heat stress. A total of 96 WLH egg type growers of 10 weeks age, were randomly divided and maintained in controlled comfort (26±1.0°C) condition and heat stressed (40±5.0°C) conditions. Each group was divided into four subgroups with twelve birds each in two replicates for homogeneity of experimental design. G1 was designated to control group whereas, G2, G3 and G4 group was supplemented with 100, 200 and 300 mg ascorbic acid (AA) respectively. Relative expression analysis of HSP70 in liver tissue was done on day 42nd of the experiment using RT-PCR technique. The mRNA expression in egg type grower birds was significantly (p<0.01) down-regulated in all the treatment groups as compared to control group in both comfort and heat stressed condition. In comfort condition, maximum down-regulation (0.32 fold) was found in G3 group followed by G4 group (0.43 fold) as compared to control group. Similarly, in heat stressed condition, maximum down-regulation (0.31 fold) was found in G3 group, followed by G4 group (0.56 fold) in comparison to control group. The present investigation reveals that supplementation of ascorbic acid on the expression patterns of HSP70 gene provide an indication that AA may be useful in combating rigors of heat stress in chickens.

Molecular Mechanism of Apoptosis in Ovary

Most ovarian follicles that begin to grow and develop fail to reach ovulatory status but instead degenerated by apoptosis (programmed cell death) of follicular cells.Ovarian follicle atresia is a common phenomenon in vertebrate ovaries and this process is characterized by follicular wall degeneration .The molecular mechanism underlying follicle atresia is apoptotic granulosa cell death Apoptosis is a genetically determined and active event, i.e., dependent on the balance of pro-and anti-apoptotic genes and requires energy. Apoptosis is mediated by active intrinsic mechanisms and extrinsic factors ,this process is conserved in terms of evolution, by which cells inactivate, disassemble and degrade their own structural and functional components systematically for completion of their own death. Ability of cells to enter in apoptosis in response to a specific death signal, depends on both their proliferative status and position in cell cycle, and on controlled expression of genes that promote, inhibit or affect cell death program.Thus, a better understanding of the mechanisms involved in atresia is necessary to avoid the great follicular loss that occurs in vivo and to maximize female reproductive potential

Effect of Alpha-Tocopherol on Biochemical Parameters in Commercial Broilers during Heat Stress

Among various environmental conditions, high ambient temperature beyond the range of the thermoneutral zone in poultry has been known as one of the most fatal stressors, which adversely affects feed intake, growth rate, immunity and mortality (Niu et al., 2009). Therefore, preventing and alleviating the heat stress (HS) against summer high ambient temperature is becoming increasingly important in the poultry industry. When chickens are exposed to high temperature, they try to reduce their body temperature within a narrow range through self-thermal regulation to maintain body homeostasis. In particular, the exposure of poultry to summer HS is stressful enough to induce their metabolic rate and physiological responses to cope with the thermal environment (Puthpongsiriporn et al., 2001). Therefore, vitamin E supplementation appears International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 10 (2017) pp. 531-539 Journal homepage: http://www.ijcmas.com

Combined effect of Emblica officinalis and Tinospora cordifolia on hemato-biochemical profile of murrah buffalo calves

The present study was aimed to assess the performance of neonatal buffalo calves based on haematological and biochemical profile upon supplementation of Emblica officinalis and Tinospora cordifolia, well known anti-anaemic, adaptogenic and anti-stressor herbs. Twelve calves aged 14 d were randomly divided into 2 groups of 6 each. One group was maintained as control and other group supplemented with a combination of dry powder of E. officinalis and T. cordifolia (2:1) @ 450 mg kg−1 body weight once a day orally for 28 days. Blood samples were collected aseptically from control and experimental animals on 28th and 42nd day age of calves from jugular vein. The treatment group showed significantly higher (p<0.05) total erythrocyte count, packed cell volume and haemoglobin concentration in blood. Total leukocyte count and L:N was significantly higher in treatment group than control group. No significant changes were observed in blood glucose, total protein, serum cholesterol and serum triglycerides during the experimental period.