Prashanta Dutta

A Micromachined High Throughput Coulter Counter for Bioparticle Detection and Counting

We describe a micromachined Coulter counter with multiple sensing microchannels for quantitative measurement of polymethacrylate particles and pollen. A unique design with sensing microelectrodes in the center of the microchannels is demonstrated. This design creates isolation resistances among channels, and thus circumvents the crosstalk caused by automatic electrical connection among microchannels. When implemented using microfluidic channels, this design is appropriate for the sensing of microscale particles in deionized water or in dilute electrolyte solution. Our design has multiple channels operating in parallel, but integrated with just one sample reservoir and one power source. The results with a four-channel device show that this device is capable of differentiating and counting micro polymethacrylate particles and Juniper pollen rapidly. Moreover, the device throughput is improved significantly in comparison to a single-channel device. The concept can be extended to a large number of sensing channels in a single chip for significant improvement in throughput.

Dielectrophoretic separation of bioparticles in microdevices: A review

In recent years, dielectrophoretic force has been used to manipulate colloids, inert particles, and biological microparticles, such as red blood cells, white blood cells, platelets, cancer cells, bacteria, yeast, microorganisms, proteins, DNA, etc. This specific electrokinetic technique has been used for trapping, sorting, focusing, filtration, patterning, assembly, and separating biological entities/particles suspended in a buffer medium. Dielectrophoretic forces acting on particles depend on various parameters, for example, charge of the particle, geometry of the device, dielectric constant of the medium and particle, and physiology of the particle. Therefore, to design an effective micro‐/nanofluidic separation platform, it is necessary to understand the role of the aforementioned parameters on particle motion. In this paper, we review studies particularly related to dielectrophoretic separation in microfluidic devices. Both experimental and theoretical works by several researchers are highlighted in this article covering AC and DC DEP. In addition, AC/DC DEP, which uses a combination of low frequency AC and DC voltage to manipulate bioparticles, has been discussed briefly. Contactless DEP, a variation of DC DEP in which electrodes do not come in contact with particles, has also been reviewed. Moreover, dielectrophoretic force‐based field flow fractionations are featured to demonstrate the bioparticle separation in microfluidic device. In numerical front, a comprehensive review is provided starting from the most simplified effective moment Stokes‐drag (EMSD) method to the most advanced interface resolved method. Unlike EMSD method, recently developed advanced numerical methods consider the size and shape of the particle in the electric and flow field calculations, and these methods provide much more accurate results than the EMSD method for microparticles.

NUMERICAL SIMULATION OF MIXED ELECTROOSMOTIC/PRESSURE DRIVEN MICROFLOWS

A spectral element algorithm is developed to analyze mixed electroosmotic/pressure driven flows in complex two-dimensional geometries. The new algorithm exhibits spectral accuracy in resolving thin electric double layers. Mixed electroosmotic/pressure driven flows are simulated in straight channels. Electrokinetic pumping and the means of producing large pressure gradients in microchannels are explored. Finally, electroosmotic flow in a T-junction geometry is analyzed under various external electric field strengths. Flowrate in the T-junction is shown to vary linearly with the electroosmotic body forces in the Stokes flow regime.

Electroosmotic flow control in complex microgeometries

Numerical simulation results for pure electroosmotic and combined electroosmotic/pressure driven Stokes flows are presented in the cross-flow and Y-split junctions. The numerical algorithm is based on a mixed structured/unstructured spectral element formulation, which results in high-order accurate resolution of thin electric double layers with discretization flexibility for complex engineering geometries. The results for pure electroosmotic flows in cross-flow junctions under multiple electric fields show similarities between the electric and velocity fields. The combined electroosmotic/pressure driven flows are also simulated by regulating the flowrate in different branches of the cross-flow junctions. Flow control in the Stokes flow regime is shown to have linear dependence on the magnitude of the externally applied electric field, both for pure electroosmotic and combined flows. This linear behavior enables utilization of electroosmotic forces as nonmechanical means of flow control for microfluidic applications.

Modeling and simulation of dielectrophoretic particle–particle interactions and assembly

Electric field induced particle-particle interactions and assembly are of great interest due to their useful applications in micro devices. The behavior of particles becomes more complex if multiple particles interact with each other at the same time. In this paper, we present a numerical study of two dimensional DC dielectrophoresis based particle-particle interactions and assembly for multiple particles using a hybrid immersed interface-immersed boundary method. The immersed interface method is employed to capture the physics of electrostatics in a fluid media with suspended particles. Particle interaction based dielectrophoretic forces are obtained using Maxwells stress tensor without any boundary or volume integration. This electrostatic force distribution mimics the actual physics of the immersed particles in a fluid media. The corresponding particle response and hydrodynamic interactions are captured through the immersed boundary method by solving the transient Navier-Stokes equations. The interaction and assembly of multiple electrically similar and dissimilar particles are studied for various initial positions and orientations. Numerical results show that in a fluid media, similar particles form a chain parallel to the applied electric field, whereas dissimilar particles form a chain perpendicular to the applied electric field. Irrespective of initial position and orientation, particles first align themselves parallel or perpendicular to the electric field depending on the similarity or dissimilarity of particles. The acceleration and deceleration of particles are also observed and analyzed at different phases of the assembly process. This comprehensive study can be used to explain the multiple particle interaction and assembly phenomena observed in experiments.

Chemically modified solid state nanopores for high throughput nanoparticle separation

The separation of biomolecules and other nanoparticles is a vital step in several analytical and diagnostic techniques. Towards this end we present a solid state nanopore-based set-up as an efficient separation platform. The translocation of charged particles through a nanopore was first modeled mathematically using the multi-ion model and the surface charge density of the nanopore membrane was identified as a critical parameter that determines the selectivity of the membrane and the throughput of the separation process. Drawing from these simulations a single 150 nm pore was fabricated in a 50 nm thick free-standing silicon nitride membrane by focused-ion-beam milling and was chemically modified with (3-aminopropyl)triethoxysilane to change its surface charge density. This chemically modified membrane was then used to separate 22 and 58 nm polystyrene nanoparticles in solution. Once optimized, this approach can readily be scaled up to nanopore arrays which would function as a key component of next-generation nanosieving systems.

Effect of baffle size, perforation, and orientation on internal heat transfer enhancement

Abstract An experimental investigation of frictional loss and heat transfer behavior of turbulent flow in a rectangular channel with isoflux heating from the upper surface is presented for different sizes, positions, and orientations of inclined baffles attached to the heated surface. Both solid and perforated baffles are used. Inclined perforated baffle combines three major heat transfer techniques, e.g. boundary layer separation, internal flow swirls, and jet impingement. Results indicate that there exists an optimum perforation density to maximize heat transfer coefficients and this optimum perforation exhibits a strong jet impingement technique from the lower confined channel along with other enhancement techniques of heat transfer.

Thermal Characteristics of Mixed Electroosmotic and Pressure-Driven Microflows

Analytical solutions for the temperature distribution, heat transfer coefficient, and Nusselt number of steady electroosmotic flows with an arbitrary pressure gradient are obtained for two-dimensional straight microchannels. The thermal analysis considers interaction among advective, diffusive, and Joule heating terms in order to obtain the thermally developing behavior of mixed electroosmotic and pressure-driven flows with isothermal boundary conditions. Heat transfer characteristics are obtained for low Reynolds number microflows where the viscous and electric field terms are very dominant. The electroosmotic component of the flow velocity is modelled with Helmholtz-Smoluchowski slip velocity, and the mixed flow velocity is presented as linear superposition of pure electroosmotic velocity and plane Poiseuille velocity. In mixed flow cases, the governing equation for energy is not separable in general. Therefore, we introduced a method that considers the extended Graetz problem. Analytical results show that the heat transfer coefficient of mixed electroosmotic and pressure-driven flow is smaller than that of pure electroosmotic flow. For the parameter range studied here (Re<0.7), the fully developed Nusselt number is independent of the thermal Peclet number for both pure electroosmotic and mixed electroosmotic-pressure driven microflows. In mixed electroosmotic and pressure-driven flows, the thermal entrance length increases with the imposed pressure gradient.

10 000-fold concentration increase of the biomarker cardiac troponin I in a reducing union microfluidic chip using cationic isotachophoresis

This paper describes the preconcentration of the biomarker cardiac troponin I (cTnI) and a fluorescent protein (R-phycoerythrin) using cationic isotachophoresis (ITP) in a 3.9 cm long poly(methyl methacrylate) (PMMA) microfluidic chip. The microfluidic chip includes a channel with a 5× reduction in depth and a 10× reduction in width. Thus, the overall cross-sectional area decreases by 50× from inlet (anode) to outlet (cathode). The concentration is inversely proportional to the cross-sectional area so that as proteins migrate through the reductions, the concentrations increase proportionally. In addition, the proteins gain additional concentration by ITP. We observe that by performing ITP in a cross-sectional area reducing microfluidic chip we can attain concentration factors greater than 10,000. The starting concentration of cTnI was 2.3 μg mL⁻¹ and the final concentration after ITP concentration in the microfluidic chip was 25.52 ± 1.25 mg mL⁻¹. To the authors knowledge this is the first attempt at concentrating the cardiac biomarker cTnI by ITP. This experimental approach could be coupled to an immunoassay based technique and has the potential to lower limits of detection, increase sensitivity, and quantify different isolated cTnI phosphorylation states.

Flight behavior of charged droplets in electrohydrodynamic inkjet printing

Flight behaviors of charged droplets are presented for electrohydrodynamic (EHD) inkjet printing. Three different kinds of EHD spraying techniques, pulsed dc, ac, and single potential (SP) ac, have been investigated and both conductive and dielectric target surfaces were considered. Experimental results show that the flight paths of charged droplets may deviate from their regular straight route, i.e., directly from the nozzle to the substrate. Depending on the droplet charge and applied electric field, droplets may deflect, reflect, or retreat to the meniscus. We can solve these drawbacks by SP EHD printing.