Prawez Alam

New phytoconstituents from the stem bark of Tinospora cordifolia Miers.

The phytochemical investigation of the stem bark of Tinospora cordifolia Miers (Menispermaceae) has afforded four new compounds, named tinosporafuranol, tinosporafurandiol, tinosporaclerodanol, and tinosporaclerodanoid, along with β-sitosterol, and their stereostructures have been elucidated correspondingly as 4-seco-cleroda-19-ol-13-furanoid, 4-seco-cleroda-6-en-18,19-diol-13-furanoid, cleroda-1(10)-en-6β-ol and cleroda-1-one-2-en-11β,15,16,18-tetraol-12,19-olide on the basis of spectral data analyses and chemical reactions.

A new HPTLC densitometric method for analysis of swertiamarin in Enicostemma littorale and commercial formulations

A high-performance thin layer chromatographic densitometric method for the analysis of swertiamarin in 60% methanolic extract of Enicostemma littorale and commercial formulations has been developed and validated in this study. Swertiamarin was separated on aluminium-backed silica gel 60 F254 plates using ethyl acetate : methanol : water (77 : 15 : 8)% v/v as the mobile phase. This system was found to give a compact spot of swertiamarin at Rf value 0.36 ± 0.01. The limit of detection and limit of quantification were found to be 31.25 and 103.12 ng spot−1, respectively. The proposed method was employed with a high degree of precision and accuracy for the estimation of swertiamarin in methanolic extract of Enicostemma littorale and in commercial formulations.

Stability-indicating densitometric HPTLC method for qualitative and quantitative analysis of hydroquinone in commercial whitening creams

High-performance thin-layer chromatographic (HPTLC) densitometric method for analysis of arbutin in commercial whitening creams was developed and validated. Aluminum-backed silica gel 60 F254 plates were used as stationary phase while methanol-chloroform-acetic acid 3.5:6:0.5 (%, v/v/v) mixture was used as mobile phase. Under these chromatographic conditions, arbutin was well separated from other ingredients. This system was found to give a well defined, sharp, and compact spot of arbutin at retention factor (RF) value of 0.40 ± 0.02. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 42.25 and 112.45 ng per spot respectively. The proposed method with high degree of precision and accuracy was employed for the analysis of arbutin both qualitatively and quantitatively in commercial whitening creams. Due to the efficiency of the method in separating arbutin from other ingredients including its degradation products, it can be applied as a stability-indicating method.

Estimation of Swertiamarin in Enicostemma Littorale and Marketed Formulations Using HPLC-UV Method

A simple, economic, reproducible, robust and precis e HPLC method for estimation of swertiamarin in both 60 % methanolic extract of Enicostemma littorale and mar- keted formulations was developed and validated in p resent investigation. The mobile phase composed of methano l and water (90:10 % v/v), gave a sharp and well-defi ned peak of swertiamarin at the retention time of 10.15 ± 1.52 min. The limit of detection (LOD) and limit of quan tifica- tion (LOQ) were 17.25 and 56.92 μ g/ml respectively. The proposed method with high degree of precision and a ccu- racy was employed for the estimation of swertiamari n in methanolic extract and in marketed formulation.

HPTLC densitometric analysis of arbutin in bulk drug and methanolic extracts of Arctostaphylos uva-ursi

A high-performance thin layer chromatographic densitometric method for the analysis of arbutin was developed and validated in the present investigation. Arbutin was separated on aluminium-backed silica gel 60 F254 plates with methanol : chloroform (3 : 7)% (v/v) as the mobile phase. This system was found to give a compact spot of arbutin at a retention factor (R f) value of 0.32 ± 0.02. The limit of detection and limit of quantification were found to be 35.42 and 106.26 ng/spot, respectively. The proposed method with a high degree of precision and accuracy was employed for the analysis of arbutin in the bulk drug and methanolic extract of Arctostaphylos uva-ursi.

New prenylated isoflavanones from the roots of Glycyrrhiza glabra

Two new prenylated isoflavanones have been isolated from the roots of Glycyrrhiza glabra L. along with the known compounds cetoleic acid, β-sitosterol, stigmasterol, lanast-5,24-dien-3β-D-glucuronopyranoside, and glucuronic acid. The structures of the prenylated isoflavanones have been established as 8-isoprenyl-7,4′-dihydroxylicoisoflavanone (glabraisoflavanone A) and 7,3′-dihydroxy-8-isoprenyl-4′-cyclogeranioloxyisoflavanone (glabraisoflavanone B) on the basis of spectral data analyses and chemical reactions.

Wound healing effects of nanoemulsion containing clove essential oil

Abstract The aim of this study was to investigate the wound healing effects of clove oil (CO) via its encapsulation into nanoemulsion. Optimized nanoemulsion (droplet size of 29.10 nm) was selected for wound healing investigation, collagen determination, and histopathological examination in rats. Optimized nanoemulsion presented significant would healing effects in rats as compared to pure CO. Nanoemulsion also presented significant enhancement in leucine content (0.61 mg/g) as compared to pure CO (0.50 mg/g) and negative control (0.31 mg/g). Histopathology of nanoemulsion treated rats showed no signs of inflammatory cells. These results suggested that nanoemulsion of CO was safe and nontoxic.

Densitometric-high-performance thin-layer chromatographic estimation of diosmin, hesperidin, and ascorbic acid in pharmaceutical formulations

An attempt was made to develop and validate a simple, accurate high-performance thin-layer chromatographic (HPTLC) method for the simultaneous determination of diosmin, hesperidin, and ascorbic acid in pharmaceutical preparations. Analysis was performed on 10 cm × 20 cm aluminum-backed plates coated with 0.2 mm layers of silica gel 60 F254 (Merck, Germany). CAMAG TLC Scanner III was used for UV densitometric scanning. The used HPTLC system was found to give sharp, symmetrical, and well-resolved peaks at RF values of 0.34 ± 0.02, 0.40 ± 0.04, and 0.56 ± 0.03, and linearity was found in the ranges 100–800 ng/spot (r2 = 0.9985), 100–800 ng/spot (r2 = 0.9966), and 50–400 ng/spot (r2 = 0.9951) for diosmin, hesperidin, and ascorbic acid, respectively.

Quantitative analysis of benzyl isothiocyanate in Salvadora persica extract and dental care herbal formulations using reversed phase C18 high-performance liquid chromatography method

Context: Benzyl isothiocyanate is the active antimicrobial agent in Salvadora persica (siwak) widely used in Islamic countries for oral hygiene. Aims: Quantification of benzyl isothiocyanate in the ethanol extract of S. persica and some dental care herbal formulations labeled to contain siwak. Settings and Design: Simple and sensitive high-performance liquid chromatography method was designed. Subjects and Methods: Separation was achieved on reverse phase C18 (250 mm × 4.6 mm, 5 μ ) column with a mobile phase comprising acetonitrile and water (1:1). The detection was carried out at 190 nm using ultra violet-visible detector. The flow rate was kept at 1 mL/min. Results: A sharp and well-defined peak was obtained at the retention time of 9.322 ± 0.3 min. Linear regression analysis data for the calibration plot showed a good linear relationship between response and concentration in the range of 0.5–500 μg/mL with a regression coefficient (r2) of 0.9977. The method was validated for accuracy, precision, robustness, and sensitivity. All the parameters examined met the current recommendations for the International Conference on Harmonization guidelines for method validation. Conclusions: The method was applied for the quantification of benzyl isothiocyanate in siwak extract, dental care powder, mouth wash, and toothpaste claimed to contain siwak. The developed method was found specific, simple, selective, and reliable for routine use in quality control analysis of different commercially available herbal care products. Abbreviations used: RP18: Reversed phase C18, HPLC: High performance liquid chromatography, UV: Ultra violet, r2: regression coefficient, ICH: international conference on harmonization, TLC: Thin layer chromatography, CHCl3: Chloroform, v/v: volume/volume, RSD: Relative standard deviation, LOD: Limit of detection, LOQ: Limit of quantification.

Quantification of glycyrrhizin biomarker in Glycyrrhiza glabra rhizome and baby herbal formulations by validated RP-HPTLC methods

Background: A simple and sensitive thin-layer chromatographic method has been established for quantification of glycyrrhizin in Glycyrrhiza glabra rhizome and baby herbal formulations by validated Reverse Phase HPTLC method. Materials and Methods: RP-HPTLC Method was carried out using glass coated with RP-18 silica gel 60 F254S HPTLC plates using methanol-water (7: 3 v/v) as mobile phase. Results: The developed plate was scanned and quantified densitometrically at 256 nm. Glycyrrhizin peaks from Glycyrrhiza glabra rhizome and baby herbal formulations were identified by comparing their single spot at Rf = 0.63 ± 0.01. Linear regression analysis revealed a good linear relationship between peak area and amount of glycyrrhizin in the range of 2000-7000 ng/band. Conclusion: The method was validated, in accordance with ICH guidelines for precision, accuracy, and robustness. The proposed method will be useful to enumerate the therapeutic dose of glycyrrhizin in herbal formulations as well as in bulk drug.