Preethi V. Udagama-Randeniya

Natural Human Antibody Responses to Plasmodium vivax Apical Membrane Antigen 1 under Low Transmission and Unstable Malaria Conditions in Sri Lanka

ABSTRACT Plasmodium vivax apical membrane antigen 1, an important malaria vaccine candidate, was immunogenic during natural malaria infections in Sri Lanka, where low transmission and unstable malaria conditions prevail. Antibody prevalence increased with exposure in areas where malaria was or was not endemic. A marked isotype switch to cytophilic (immunoglobulin G1 [IgG1]/IgG3) antibodies was evident with increasing exposure exclusively in residents from areas of endemicity.

Genetic diversity and recombination at the C-terminal fragment of the merozoite surface protein-1 of Plasmodium vivax (PvMSP-1) in Sri Lanka

Extensive polymorphism in the genes encoding for surface antigens of Plasmodium falciparum and Plasmodium vivax has been a serious impediment for malaria vaccine development. One such antigen is the merozoite surface protein-1 (MSP-1). The MSP-1 precursor after proteolytic cleavage generates a C-terminal fragment of 42 kDa (MSP-1(42)), which subsequently produces 33 kDa (MSP-1(33)) and 19 kDa (MSP-1(19)) fragments. Since MSP-1(42) is currently being considered as a candidate for vaccine development against blood stage malaria it is important to catalogue the existing diversity in this antigen in natural P. vivax infections. Here we investigated the level of genetic diversity in the PvMSP-1(42) gene fragment in 95 single clone P. vivax infections in Sri Lanka. We observed that the PvMSP-1(19) fragment was highly conserved among these samples, whereas the PvMSP-1(33) fragment exhibited extensive diversity with 39 polymorphic amino acid positions (corresponding to 27 haplotypes, 19 of which were unique to Sri Lanka). Of these 27 PvMSP-1(42) haplotypes, 24 belonged to hypervariable region (HVR) T1-T7 types, while 3 haplotypes were generated by interallelic recombination between T1/T3 (HVRT8-T9) and T2/T3 (HVRT10). In addition, we analysed 107 PvMSP-1(42) sequences (corresponding to 62 haplotypes, H28 to H89) deposited in the NCBI GenBank database from other regions of the world. Seventy-four of these correspond to 9 of the 10 HVR types (HVR-T7 was unique to Sri Lanka). Two novel HVR types, T11 and T12, with a double recombination between HVR-T1/T3 and HVRT6/T2, were derived from South America and Thailand, respectively. T cell epitope polymorphism arising due to non-synonymous substitutions in PvMSP-1(33) may result in differential binding of the polymorphic peptides to class II MHC alleles, inducing different host immune responses. In conclusion, under low transmission and unstable malaria conditions prevalent in Sri Lanka, extensive allelic polymorphism was evident at PvMSP-1(33) due to recombination, mutation, and balancing selection. In contrast, PvMSP-1(19) is highly conserved(,) greatly enhancing its suitability as a malaria vaccine candidate.

Two new species of chiggers (Acari: Leeuwenhoekiidae and Trombiculidae) from bats (Chiroptera) collected in the Kanneliya forest reserve of Sri Lanka

Abstract Ectoparasitic mites collected from three Hipposideros speoris (Schneider, 1800) hosts (Chiroptera: Hipposideridae) taken from bat habitats in the Kanneliya Forest Reserve of Sri Lanka has revealed the presence of two new species of chiggers, one in the genus Whartonia in the Leeuwenhoekiidae and one in the genus Chiroptella (Neosomia) in the Trombiculidae. Descriptions and remarks are presented.