Premlata Singariya

In vitro Studies of Antimicrobial Activity of Crude Extracts of the Indian Grasses Dhaman (Cenchrus ciliaris) and Kala-Dhaman (Cenchrus setigerus)

The aim of present study was to investigate the antimicrobial activity of Cenchrus ciliaris and Cenchrus setigerus extracts in order to use it as a possible source for new antimicrobial substances against important human pathogens. Crude extracts of the stem of Cenchrus ciliaris and Cenchrus setigerus were evaluated against some medically important pathogens viz. Escherichia coli, Raoultella planticola, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Enterobacter aerogenes, Candida albicans and Aspergillus flavus. The dried and powdered stems were successively extracted with hexane, toluene, isopropyl alcohol, acetone and ethanol using soxhlet assembly. The antimicrobial activity assay was done by both disc diffusion and serial dilution methods. Isopropyl alcohol extract of Cenchrus setigerus showed highest activity against Escherichia coli. The test pathogens were more sensitive to the isopropyl alcohol, acetone and ethanol extracts than to the hexane and toluene extracts except against Bacillus subtilis. Result reveals that the most bioactive compound was cycloleucolenol-9,19-cycloergost-24 (28)-en-3-ol, 4, 14-dimethyl acetate in both the species of Cenchrus grass, (19.15%) in isopropanol extract of Cenchrus setigerus whereas, (14.03%) in acetone extract of Cenchrus ciliaris.

Absence of antibiotic activities of Cenchrus setigerus and cenchrus ciliaris seed extracts in different polar solvents

Aim: The aim of present study is to investigate the antibiotic activity of seeds of Cenchrus setigerus and Cenchrus ciliaris extracts, in order to use them as a possible source of new anti-microbial substances against important human pathogens. Materials and Methods: Crude extracts of seeds of both species of Cenchrus grass were evaluated against some important G-ve bacteria. Escherichia coli, Raoultella planticola, Enterobacter aerogenes and one fungus Aspergillus flavus. The dried and powdered seeds were successively extracted with hexane, toluene, isopropyl alcohol, acetone and ethanol using the soxhlet assembly. The antimicrobial activity assay was done by both disc diffusion and serial dilution methods. Results: The results indicate that all the extracts, in different polar solvents did not show any antibacterial activity against R. planticola or any antifungal activity against A. flavus. Conclusion: All extracts in the different polar solvents did not have or had very less antibacterial and antifungal activities.

Insignificant antimicrobial activities and phytochemical screening in different extracts of indian Ginseng

Aim: The aim of present study is to investigate the antimicrobial activity of (Flower, Ripen fruit and calyx) of Withania somnifera (RUBL20668) extracts, in order to use them as a possible source of new antimicrobial substances against important human pathogens. Materials and Methods: Crude extracts of different parts of both species of W. somnifera were evaluated against some important bacteria (two Gram +ve and four Gram-ve bacteria). Staphylococcus aureus (Gram +ve), Bacillus Subtilis (Gram +ve), Escherichia coli (Gram-ve), Raoultella planticola (Gram-ve), Pseudomonas aeruginosa (Gram-ve), Enterobactor aerogens (Gram-ve), one yeast Candida albicans and one fungus Aspergillus flavus. The dried and powdered seeds were successively extracted with hexane, toluene, iso propyl alcohol, acetone and ethanol, using the soxhlet assembly. The antimicrobial activity assay was done by both disc diffusion and serial dilution methods. Result: The results indicate that all the extracts in different polar solvents did not show any antibacterial activity against S. aureus, E. coli, R. planticola or E. aerogens or any antifungal activity against A. flavus. Conclusion: All extracts in the different polar solvents did not have or had very less antibacterial and antifungal activities.